pLV3-CMV-HA-UB-mCherry-Puro plasmid

PVT101053

pLV3-CMV-HA-UB-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-HA-UB(mouse)-mCherry-Puro
Alias: -
Gene lenght: 231bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-HA-UB-mCherry-Puro plasmid Description

pLV3-CMV-HA-UB-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HA-UB-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 1981 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 2041 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 2101 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 2161 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 2221 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 2281 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 2341 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 2401 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga 2461 tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt 2521 accgcgggcc cgggatccgc caccatgTAC CCATACGATG TTCCAGATTA CGCTatgcag 2581 atctttgtga aaaccttaac tggtaagacc atcaccctgg aggtcgagcc cagtgacacc 2641 attgagaatg tcaaggcaaa gatccaggac aaggagggca tcccccctga ccagcagagg 2701 ctgatctttg caggcaagca gctggaagat ggccgcaccc tgtcagacta caacatccag 2761 aaagagtcca ccctgcacct ggtccttcgc ctcagaggtg gcTAGgcggc cgcGaaggat 2821 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 2881 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 2941 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 3001 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 3061 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 3121 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 3181 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 3241 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 3301 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 3361 gctagacgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 3421 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 3481 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3541 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 3601 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 3661 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 3721 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 3781 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 3841 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 3901 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 3961 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 4021 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 4081 gggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg 4141 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc 4201 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 4261 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 4321 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 4381 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 4441 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 4501 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 4561 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 4621 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 4681 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 4741 ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4801 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4861 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4921 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4981 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5041 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5101 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5161 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5221 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5281 tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc 5341 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5401 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg 5461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 5581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 5641 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 5701 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 5761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 5821 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc 5881 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 5941 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 6001 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 6061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 6121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 6181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 6241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 6301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 6361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 6421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 6481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6841 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 6901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 7021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 7081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 7141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 7201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 7261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 7321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 7381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 7441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 7501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 7561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 7621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 7681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 7741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 8101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 8161 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 8221 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 8281 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 8341 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 8401 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 8461 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 8521 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 8581 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 8641 gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???