pLV3-CMV-HNF1B-3×FLAG-CopGFP-Puro plasmid

PVT96826

pLV3-CMV-HNF1B-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HNF1B(human)-3×FLAG-CopGFP-Puro
Alias: NM_000458.4;ADTKD3; FJHN; HNF-1-beta; HNF-1B; HNF1beta;HNF2; HPC11; LF-B3; LFB3; MODY5; RCAD; T2D; TCF-2; TCF2;VHNF1
Gene lenght: 1671bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HNF1B-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HNF1B-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HNF1B-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg tgtccaagct cacgtcgctc 2341 cagcaagaac tcctgagcgc cctgctgagc tccggggtca ccaaggaggt gctggttcag 2401 gccttggagg agttgctgcc atccccgaac ttcggggtga agctggagac gctgcccctg 2461 tcccctggca gcggggccga gcccgacacc aagccggtct tccatactct caccaacggc 2521 cacgccaagg gccgcttgtc cggcgacgag ggctccgagg acggcgacga ctatgacaca 2581 cctcccatcc tcaaggagct gcaggcgctc aacaccgagg aggcggcgga gcagcgggcg 2641 gaggtggacc ggatgctcag tgaggaccct tggagggctg ctaaaatgat caagggttac 2701 atgcagcaac acaacatccc ccagagggag gtggtcgatg tcaccggcct gaaccagtcg 2761 cacctctccc agcatctcaa caagggcacc cctatgaaga cccagaagcg tgccgctctg 2821 tacacctggt acgtcagaaa gcaacgagag atcctccgac aattcaacca gacagtccag 2881 agttctggaa atatgacaga caaaagcagt caggatcagc tgctgtttct ctttccagag 2941 ttcagtcaac agagccatgg gcctgggcag tccgatgatg cctgctctga gcccaccaac 3001 aagaagatgc gccgcaaccg gttcaaatgg gggcccgcgt cccagcaaat cttgtaccag 3061 gcctacgatc ggcaaaagaa ccccagcaag gaagagagag aggccttagt ggaggaatgc 3121 aacagggcag aatgtttgca gcgaggggtg tccccctcca aagcccacgg cctgggctcc 3181 aacttggtca ctgaggtccg tgtctacaac tggtttgcaa accgcaggaa ggaggaggca 3241 ttccggcaaa agctggccat ggacgcctat agctccaacc agactcacag cctgaaccct 3301 ctgctctccc acggctcccc ccaccaccag cccagctcct ctcctccaaa caagctgtca 3361 ggagtgcgct acagccagca gggaaacaat gagatcactt cctcctcaac aatcagtcac 3421 catggcaaca gcgccatggt gaccagccag tcggttttac agcaagtctc cccagccagc 3481 ctggacccag gccacaatct cctctcacct gatggtaaaa tgatctcagt ctcaggagga 3541 ggtttgcccc cagtcagcac cttgacgaat atccacagcc tctcccacca taatccccag 3601 caatctcaaa acctcatcat gacacccctc tctggagtca tggcaattgc acaaagcctc 3661 aacacctccc aagcacagag tgtccctgtc atcaacagtg tggccggcag cctggcagcc 3721 ctgcagcccg tccagttctc ccagcagctg cacagccctc accagcagcc cctcatgcag 3781 cagagcccag gcagccacat ggcccagcag cccttcatgg cagctgtgac tcagctgcag 3841 aactcacaca tgtacgcaca caagcaggaa cccccccagt attcccacac ctcccggttt 3901 ccatctgcaa tggtggtcac agataccagc agcatcagta cactcaccaa catgtcttca 3961 agtaaacagt gtcctctaca agcctgggga tccGACTACA AAGACCATGA CGGTGATTAT 4021 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 4081 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 4141 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 4201 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 4261 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 4321 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 4381 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 4441 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 4501 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 4561 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4621 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 4681 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 4741 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 4801 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 4861 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 4921 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 4981 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 5041 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 5101 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 5161 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 5221 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 5281 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 5341 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 5401 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 5461 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 5521 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 5581 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 5641 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 5701 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 5761 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5821 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5881 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5941 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 6001 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 6061 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 6121 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 6181 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 6241 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 6301 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 6361 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 6421 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 6481 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 6541 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 6601 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 6661 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 6721 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 7141 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 7201 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 7261 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 7321 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 8041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 8101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 8161 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 9001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 9061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 9121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9481 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9541 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9661 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9721 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9781 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9841 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9901 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 9961 g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???