pLV3-CMV-Itga1-3×FLAG-CopGFP-Puro plasmid

PVT92418

pLV3-CMV-Itga1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Itga1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001033228.3;CD49A; E130012M19Rik; Vla1;
Gene lenght: 3537bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Itga1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Itga1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Itga1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg tccccaggcg tcctgctagc 2341 ctagaggtca ctgtagcctg catctggctt ctcaccgtta tcctaggcgt ctgcatctcc 2401 ttcaacgttg atgtcaaaaa ctccatgagc ttcagtggtc cagtggagga catgtttgga 2461 tacaccgttc aacagtatga aaacgaagaa ggcaaatggg ttcttattgg ttctccttta 2521 gttggccaac ccaaagcaag aactggagat gtctataagt gtccagttgg gagagagaga 2581 tcaatgcctt gtgtgaagtt ggatttacca gttaacacat cgattcctaa tgtcacagaa 2641 ataaaggaaa acatgacatt tggatcaact ttagtcacca atccgaaggg aggatttctg 2701 gcatgtgggc ccttgtatgc ctatagatgt ggacatttgc attatacgac tggcatatgt 2761 tctgatgtca gccctacatt tcaagttgtg aactcgttcg cccctgtaca agaatgtagc 2821 acacagcttg acatagtcat cgtcctagat ggctccaaca gcatctaccc ctgggaaagt 2881 gtcactgcct ttttaaacga ccttcttaag aggatggaca ttggccccaa acagacacag 2941 gttggaattg tacaatatgg agcaaatgta acacatgagt tcaacctcaa taagtattca 3001 tccacagaag aggtcctggt ggcagcaaac aaaataggcc gcaggggtgg tctccagacg 3061 atgacagccc ttggaataga cacagccagg aaagaggcat tcactgaagc tcggggggcc 3121 agaaggggag tgaaaaaagt catggttatt gtgactgatg gagaatcgca tgacaactat 3181 cgcctgaaac aggtcatcca agactgtgag gatgaaaaca ttcagcgatt ttccatagct 3241 atccttggcc actataacag ggggaactta agcaccgaaa aatttgtgga ggagataaaa 3301 tcaatcgcaa gtgagcccac cgaaaagcac ttcttcaacg tctcagatga gttggccctg 3361 gtcactattg ttaaagctct gggagaaagg atatttgctt tggaagctac agctgaccag 3421 tcagcagctt cgtttgaaat ggagatgtct cagaccggct tcagtgctca ttattcacag 3481 gactgggtca tgcttggagc tgttggagcc tatgactgga acggaactgt ggtcatgcag 3541 aaggctaacc agattgtcat ccctcataac accacctttc aaactgagcc caccaagatg 3601 aacgagcctc ttgcttctta cttgggttac acagtgaact ctgccaccat cccgggagat 3661 gtgctctata tcgctgggca gcctcggtac aatcacaccg gccaggtcgt catctacaag 3721 atggaggatg gggacgtcaa cattctgcag acactcagtg gagagcagat cggatcctac 3781 tttggtagcg tcttaaccac aattgacatc gacaaagatt cttacactga tctgcttctc 3841 gtcggggctc ccatgtatat ggggacagag aaagaggaac agggcaaggt gtacgtgtac 3901 gctgtgaatc agacgaggtt tgaatatcaa atgagcctgg aaccaattaa gcagacctgc 3961 tgttcatccc tgaaggataa ttcatgcaca aaagaaaaca agaatgagcc ctgtggggcc 4021 cgttttggaa cagcagttgc tgctgtaaaa gacctcaacg tggatggctt taatgacgtc 4081 gtgattggag ctcccctgga agacgaccac gcaggagctg tgtacattta tcatggcagt 4141 ggcaagacca taaggaaaga gtatgcgcaa cgcattccct caggtgggga tgggaagacg 4201 ctgaaatttt ttggccagtc tatccacgga gagatggatt taaatggcga tggtctgact 4261 gacgtgacca ttggaggcct tggaggagct gccctcttct gggccagaga tgtggctgta 4321 gttaaagtga ccatgaattt tgaacccaac aaagtgaata ttcaaaagaa aaactgccgt 4381 gtggagggca aagaaacagt atgcataaat gctacaatgt gttttcatgt gaaattgaag 4441 tctaaagaag actcagttta cgaggctgat ctgcagtacc gtgtcaccct tgattcgctg 4501 aggcagatat cacggagctt tttttctgga actcaggaaa ggaggattca aagaaacctc 4561 accgttcgag aatccgaatg catcaggcac tccttctaca tgttggataa gcacgacttt 4621 caggactccg tgagagtgac tttggatttt aacctcactg atccagaaaa tgggcccgtg 4681 ctcgatgacg ctctgccaaa ctcagtccat ggacatattc cttttgccaa agactgtggg 4741 aacaaggaaa gatgcgtttc agacctcacc ctggatgtgt ccacaacaga aaagaacctg 4801 ctgattgtca gatcccagaa tgacaagttc aatgtcagcc tcaccgtcaa aaacaaggga 4861 gacagtgcgt acaacacccg gacagtggtt cagtattctc caaatctgat tttttcagga 4921 attgaggaga tccaaaaaga tagctgcgaa tccaatcaaa atatcacgtg tagagttgga 4981 tatcctttcc tgaggacagg agacgtggtt aacttcaaaa taatattcca gtttaataca 5041 tcacatctct cagaaaatgc aattattcat ttaagtgcaa caagtgacag tgaagagccc 5101 ctggaatctc tttacgataa tgaagtaaat atttccatcc ccgtaaaata tgaagtcgga 5161 ctgcagtttt acagttctgc gagtgaacac cacatttcag ttgctgccaa tgagactgtt 5221 cctgagctga ttaattccac caaggacatt ggggatgaaa ttaatgtctt ctacacgatt 5281 agaaaaagag ggcatttccc aatgccagaa cttcggctgg caatttcatt tcccaatctg 5341 acgtcagatg gctatcctgt actgtaccca actggatggt catcttctga taatgtaaac 5401 tgcagacctc gaagccttga ggaccctttg ggtatcaact ctgggaagaa aatgacaata 5461 tcaaagtctg aggttctcaa aagaggcaca atccaggact gcagtacctg caagattgct 5521 accatcacgt gtcatctcct tccctcggat gtgagtcaag tgaatgtctc actcatcttg 5581 tggaaaccaa ctttcataaa agcacatttt tccagcttaa atcttaccat acggggagaa 5641 cttcagagtg aaaactcatc gctgacttta agcagcagca accggaaacg agagctggct 5701 attcagatat ccaaagacgg gctcccaggc cgagtgccgc tgtgggttat cctcctgagc 5761 gcctttgcgg gactcctgct gttaatgctg cttatattag ctctgtggaa gattggattc 5821 ttcaaaaggc cactaaagaa gaaaatggag aaaggatccG ACTACAAAGA CCATGACGGT 5881 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 5941 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 6001 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 6061 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 6121 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 6181 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 6241 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 6301 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 6361 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 6421 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 6481 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 6541 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 6601 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 6661 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 6721 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 6781 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 6841 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 6901 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 6961 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 7021 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 7081 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 7141 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 7201 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 7261 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 7321 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 7381 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 7441 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 7501 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 7561 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 7621 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 7681 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 7741 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 7801 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 7861 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 7921 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 7981 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 8041 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 8101 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 8161 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 8221 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 8281 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 8341 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 8401 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 8461 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 8521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 8581 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 8641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 8701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 8821 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 8881 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 8941 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 9001 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 9061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 9121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 9181 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 9241 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 9301 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 9361 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 9421 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 9481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 9541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 9601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 9661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 9721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 9781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 9841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 9901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 9961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 10021 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 10081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 10141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 10201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 10261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 10321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 10381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 10441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 10501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 10561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 10621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 10681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 10741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 10801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 10861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 10921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 10981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 11041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 11101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 11161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 11221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 11281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 11341 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 11401 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 11461 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 11521 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 11581 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 11641 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 11701 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 11761 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 11821 caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???