pLV3-CMV-ITM2C-mCherry-Puro plasmid

PVT101064

pLV3-CMV-ITM2C-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-ITM2C(human)-mCherry-Puro
Alias: NM_030926.6;BRI3; BRICD2C; E25; E25C; ITM3
Gene lenght: 801bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ITM2C-mCherry-Puro plasmid Description

pLV3-CMV-ITM2C-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ITM2C-mCherry-Puro plasmid Sequence

1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 361 gcgctaccgg ACTCAGATCT CGAGCTCAAG CTTCGAATTC TGCAGTCGAC gccaccatgg 421 tgaagattag cttccagccc gccgtggctg gcatcaaggg cgacaaggct gacaaggcgt 481 cggcgtcggc ccctgcgccg gcctcggcca ccgagatcct gctgacgccg gctagggagg 541 agcagccccc acaacatcga tccaagaggg ggggctcagt gggcggcgtg tgctacctgt 601 cgatgggcat ggtcgtgctg ctcatgggcc tcgtgttcgc ctctgtctac atctacagat 661 acttcttcct tgcgcagctg gcccgagata acttcttccg ctgtggtgtg ctgtatgagg 721 actccctgtc ctcccaggtc cggactcaga tggagctgga agaggatgtg aaaatctacc 781 tcgacgagaa ctacgagcgc atcaacgtgc ctgtgcccca gtttggcggc ggtgaccctg 841 cagacatcat ccatgacttc cagcggggtc tgactgcgta ccatgatatc tccctggaca 901 agtgctatgt catcgaactc aacaccacca ttgtgctgcc ccctcgcaac ttctgggagc 961 tcctcatgaa cgtgaagagg gggacctacc tgccgcagac gtacatcatc caggaggaga 1021 tggtggtcac ggagcatgtc agtgacaagg aggccctggg gtccttcatc taccacctgt 1081 gcaacgggaa agacacctac cggctccggc gccgggcaac gcggaggcgg atcaacaagc 1141 gtggggccaa gaactgcaat gccatccgcc acttcgagaa caccttcgtg gtggagacgc 1201 tcatctgcgg ggtggtgggt ggaggcggat caggaggcgg tggatcACCG GTCGCCACCa 1261 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg 1321 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 1381 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct 1441 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc 1501 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg 1561 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg 1621 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 1681 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg 1741 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg 1801 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca 1861 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac 1921 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtGA Attctcgacc 1981 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac 2041 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 2101 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt 2161 tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca 2221 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg 2281 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc 2341 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg 2401 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca 2461 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg 2521 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt 2581 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc 2641 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga 2701 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 2761 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 2821 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 2881 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 2941 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 3001 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 3061 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 3121 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 3181 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 3241 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 3301 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc 3361 atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 3421 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 3481 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 3541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 3601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 3661 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 3721 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 3781 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 3841 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 3901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 3961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 4021 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc 4081 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 4141 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 4201 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 4261 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 4321 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 4381 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 4441 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 4501 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 4561 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 4621 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 4681 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 4741 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 4801 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 4861 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 4921 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 4981 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 5041 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 5101 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 5161 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 5221 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 5281 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 5341 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 5401 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 5461 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 5521 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 5581 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 5641 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 5701 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5761 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 5821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 5881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 5941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 6001 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 6061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 6121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 6181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 6241 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 6301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 6361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 6421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 6481 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 6541 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 6601 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 6661 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 6721 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 6781 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 6841 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 6901 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg 6961 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa 7021 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga 7081 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata 7141 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 7201 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 7261 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 7321 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 7381 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 7441 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 7501 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 7561 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 7621 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 7681 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 7741 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 7801 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 7861 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 7921 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 7981 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 8041 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 8101 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 8161 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 8221 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 8281 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 8341 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 8401 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 8461 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 8521 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 8581 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 8641 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 8701 cgacggtatc gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt 8761 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt 8821 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt 8881 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???