pLV3-CMV-KCNC2-3×FLAG-CopGFP-Puro plasmid

PVT112069

pLV3-CMV-KCNC2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KCNC2(human)-3×FLAG-CopGFP-Puro
Alias: NM_139136.4;DEE103; KV3.2
Gene lenght: 1839
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KCNC2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-KCNC2-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KCNC2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcaagatcga gaacaacgag 2341 agggtgatcc tcaatgtcgg gggcacccgg cacgaaacct accgcagcac cctcaagacc 2401 ctgcctggaa cacgcctggc ccttcttgcc tcctccgagc ccccaggcga ctgcttgacc 2461 acggcgggcg acaagctgca gccgtcgccg cctccactgt cgccgccgcc gagagcgccc 2521 ccgctgtccc ccgggccagg cggctgcttc gagggcggcg cgggcaactg cagttcccgc 2581 ggcggcaggg ccagcgacca tcccggtggc ggccgcgagt tcttcttcga ccggcacccg 2641 ggcgtcttcg cctatgtgct caattactac cgcaccggca agctgcactg ccccgcagac 2701 gtgtgcgggc cgctcttcga ggaggagctg gccttctggg gcatcgacga gaccgacgtg 2761 gagccctgct gctggatgac ctaccggcag caccgcgacg ccgaggaggc gctggacatc 2821 ttcgagaccc ccgacctcat tggcggcgac cccggcgacg acgaggacct ggcggccaag 2881 aggctgggca tcgaggacgc ggcggggctc gggggccccg acggcaaatc tggccgctgg 2941 aggaggctgc agccccgcat gtgggccctc ttcgaagacc cctactcgtc cagagccgcc 3001 aggtttattg cttttgcttc tttattcttc atcctggttt caattacaac tttttgcctg 3061 gaaacacatg aagctttcaa tattgttaaa aacaagacag aaccagtcat caatggcaca 3121 agtgttgttc tacagtatga aattgaaacg gatcctgcct tgacgtatgt agaaggagtg 3181 tgtgtggtgt ggtttacttt tgaattttta gtccgtattg ttttttcacc caataaactt 3241 gaattcatca aaaatctctt gaatatcatt gactttgtgg ccatcctacc tttctactta 3301 gaggtgggac tcagtgggct gtcatccaaa gctgctaaag atgtgcttgg cttcctcagg 3361 gtggtaaggt ttgtgaggat cctgagaatt ttcaagctca cccgccattt tgtaggtctg 3421 agggtgcttg gacatactct tcgagctagt actaatgaat ttttgctgct gataattttc 3481 ctggctctag gagttttgat atttgctacc atgatctact atgccgagag agtgggagct 3541 caacctaacg acccttcagc tagtgagcac acacagttca aaaacattcc cattgggttc 3601 tggtgggctg tagtgaccat gactaccctg ggttatgggg atatgtaccc ccaaacatgg 3661 tcaggcatgc tggtgggagc cctgtgtgct ctggctggag tgctgacaat agccatgcca 3721 gtgcctgtca ttgtcaataa ttttggaatg tactactcct tggcaatggc aaagcagaaa 3781 cttccaagga aaagaaagaa gcacatccct cctgctcctc aggcaagctc acctactttt 3841 tgcaagacag aattaaatat ggcctgcaat agtacacaga gtgacacatg tctgggcaaa 3901 gacaatcgac ttctggaaca taacagatca gtgttatcag gtgacgacag tacaggaagt 3961 gagccgccac tatcaccccc agaaaggctc cccatcagac gctctagtac cagagacaaa 4021 aacagaagag gggaaacatg tttcctactg acgacaggtg attacacgtg tgcttctgat 4081 ggagggatca ggaaagataa ctgcaaagag gttgtcatta ctggttacac gcaagccgag 4141 gccagatctc ttactggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 4201 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 4261 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4321 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4381 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4441 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4501 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4561 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4621 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4681 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4741 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4801 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4861 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4921 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4981 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 5041 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 5101 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 5161 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 5221 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 5281 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 5341 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5401 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5461 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5521 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 5581 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5641 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5701 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5761 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5821 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5881 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5941 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 6001 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 6061 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6121 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6181 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 6241 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6301 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6361 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6421 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6481 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6541 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6601 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6661 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6721 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6781 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6841 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6901 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7081 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7141 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 7201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 7321 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7441 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7501 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7561 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7621 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7681 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9661 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9721 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9781 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9841 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9901 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9961 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 10021 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 10081 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.