pLV3-CMV-LPCAT1-3×FLAG-CopGFP-Puro plasmid

PVT93909

pLV3-CMV-LPCAT1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LPCAT1(human)-3×FLAG-CopGFP-Puro
Alias: NM_024830.5;AGPAT10; AGPAT9; AYTL2; lpcat; LPCAT-1;LPLAT8; lysoPAFAT; PFAAP3;
Gene lenght: 1602bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LPCAT1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-LPCAT1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-LPCAT1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga ggctgcgggg atgcggaccc 2341 cgggccgccc ctgcctccag cgcaggggcc agcgacgctc ggctgctggc gcccccgggg 2401 cggaacccct tcgtgcacga gctgcgcctc agcgccctgc agaaggccca ggtggccctc 2461 atgacactga cgctcttccc ggtccggctc ctggttgccg ctgccatgat gctgctggcc 2521 tggcccctcg cacttgtcgc atccctgggc tctgcggaga aggaacccga gcagcccccg 2581 gccctgtgga ggaaggttgt ggacttcctg ctgaaggcca tcatgcgcac catgtggttc 2641 gccggcggct tccaccgggt ggccgtgaag gggcggcagg cgctgcccac cgaggcggcc 2701 atcctcacgc tcgcgcctca ctcgtcctac ttcgacgcca tccctgtgac catgacgatg 2761 tcctccatcg tgatgaaggc agagagcaga gacatcccga tctggggaac tctgatccag 2821 tatatacggc ctgtgttcgt gtcccggtca gaccaggatt ctcgcaggaa aacagtagaa 2881 gaaatcaaga gacgggcgca gtccaacgga aagtggccac agataatgat ttttccagaa 2941 ggaacttgta caaacaggac ctgcctaatt accttcaaac ctggtgcatt catccctgga 3001 gcgcccgtcc agcctgtggt tttacgatat ccaaataaac tggacaccat cacatggacg 3061 tggcaaggac ctggagcgct ggaaatcctg tggctcacgc tgtgtcagtt tcacaaccaa 3121 gtggaaatcg agttccttcc tgtgtacagc ccttctgagg aggagaagag gaaccccgcg 3181 ctgtatgcca gcaacgtgcg gcgagtcatg gccgaggcct tgggtgtctc cgtgactgac 3241 tacacgttcg aggactgcca gctggccctg gcggaaggac agctccgtct ccccgctgac 3301 acttgccttt tagaatttgc caggctcgtg cggggcctcg ggctaaaacc agaaaagctt 3361 gaaaaagatc tggacagata ctcagaaaga gccaggatga agggaggaga gaagataggt 3421 attgcggagt ttgccgcctc cctggaagtc cccgtttctg acttgctgga agacatgttt 3481 tcactgttcg acgagagcgg cagcggcgag gtggacctgc gagagtgtgt ggttgccctg 3541 tctgtcgtct gccggccggc ccggaccctg gacaccatcc agctggcttt caagatgtac 3601 ggagcgcaag aggacggcag cgtcggcgaa ggtgacctgt cctgcatcct caagacggcc 3661 ctgggggtgg cagagctcac cgtgaccgac ctattccgag ccattgacca agaggagaag 3721 gggaagatca cattcgctga cttccacagg tttgcagaaa tgtaccctgc cttcgcagag 3781 gaatacctgt acccggatca gacacatttc gaaagctgtg cagagacctc acctgcgcca 3841 atcccaaacg gcttctgtgc cgatttcagc ccggaaaact cagacgctgg gcggaagcct 3901 gttcgcaaga agctggatgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3961 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 4021 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4081 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4141 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4201 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4261 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4321 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4381 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4441 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4501 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4561 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4621 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4681 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4741 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4801 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4861 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4921 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4981 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 5041 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 5101 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5161 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5221 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5281 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5341 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5401 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5461 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5521 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5581 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5641 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5701 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5761 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5821 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5881 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5941 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 6001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6361 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6481 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6541 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6601 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6661 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6721 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6781 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6841 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6901 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 7021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 7081 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 7141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7201 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7261 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7321 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7381 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8101 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 9001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 9061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9421 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9481 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9541 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9601 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9661 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9721 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9781 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9841 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???