pLV3-CMV-LRPPRC-3×FLAG-CopGFP-Puro plasmid

PVT104561

pLV3-CMV-LRPPRC-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LRPPRC(human)-3×FLAG-CopGFP-Puro
Alias: NM_133259.4;CLONE-23970; GP130; LRP130; LSFC; MC4DN5
Gene lenght: 4182
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LRPPRC-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-LRPPRC-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-LRPPRC-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cagccctgct gagatccgcg 2341 cgttggttgc tgcgtgccgg ggcggccccg cgcctcccgc tctccctgcg cctcctccct 2401 ggcggcccgg gccggctgca tgccgcctcc tatctgcccg ccgctcgcgc cgggcccgtg 2461 gccggaggac tactgagccc agccaggctg tatgccattg ctgccaaaga aaaagatatt 2521 caagaggagt ccactttttc ttctaggaag atttccaatc agtttgattg ggctctaatg 2581 agactagatc tttctgttcg aagaactggc cgcattccaa agaagcttct acaaaaagtt 2641 tttaatgata cctgccgctc aggtggccta ggtggtagtc atgccttgct tctactacgt 2701 agttgtggtt ctctcttgcc tgaactaaag cttgaagaga gaacagaatt tgctcatagg 2761 atatgggaca cacttcagaa attaggtgct gtgtatgatg tgagtcacta taatgcttta 2821 cttaaagtct atcttcaaaa tgaatataaa ttctcaccaa ctgatttcct ggcaaaaatg 2881 gaggaagcaa acattcaacc aaatcgagtg acataccaga gattgattgc ttcttattgt 2941 aatgtaggag atattgaagg tgccagcaag attcttggat ttatgaaaac taaggatctc 3001 ccagttacag aggcagtatt cagtgccctt gtgacagggc atgccagagc tggtgatatg 3061 gagaatgcag aaaacattct cacagtgatg agagatgccg gaattgagcc tggtccagac 3121 acatacctcg cattattgaa tgcatatgct gagaagggcg acattgacca tgttaagcag 3181 actctggaga aggtggagaa gtccgagctt caccttatgg accgtgattt actgcaaatt 3241 atttttagct tcagtaaagc tgggtatcct cagtatgtct cagaaatttt ggaaaaagtt 3301 acatgtgaaa gaagatatat tccagatgca atgaacctca ttttactttt agtcactgaa 3361 aaattggaag atgtagcgtt gcaaatttta ctagcatgcc ccgtatcaaa ggaagatggc 3421 ccaagtgtct ttggcagttt ctttttacaa cactgtgtga ctatgaatac gcctgtggag 3481 aagctaacag actactgtaa gaagttaaag gaagtccaga tgcactcctt tcctctgcag 3541 ttcaccctcc attgtgcttt actcgccaat aaaactgatt tggcaaaagc cttaatgaag 3601 gctgtgaagg aggaaggttt tcctatcaga cctcactatt tctggccatt gctagttgga 3661 cgtcggaagg aaaaaaatgt tcaaggtata attgaaatcc tcaaaggaat gcaagaattg 3721 ggagtacatc ctgatcagga aacatataca gattatgtga ttccatgctt tgatagtgta 3781 aactcagcac gagccatttt gcaggaaaat ggatgtctgt ctgatagtga tatgttttct 3841 caagctggat tgagaagtga agcagcaaat gggaacttag actttgtatt atcatttttg 3901 aaatcaaata cattgcccat ctcgctgcag tctataagaa gtagcctact gctaggcttc 3961 aggaggtcta tgaatataaa tctttggagc gagataacag aattgttgta caaggatgga 4021 cgttattgcc aggagcctcg aggaccgacg gaagctgttg gctattttct ttataacttg 4081 attgacagca tgagtgactc agaggtacag gccaaggagg agcatttgag acaatacttc 4141 catcagctgg agaagatgaa tgtaaaaatt cctgaaaata tctacagagg cattcgtaat 4201 ctcctggaaa gctaccatgt tcctgaattg attaaggatg ctcacttgtt ggttgagagt 4261 aagaatttag actttcaaaa aactgtgcaa cttacatcat ctgaattgga gtccacactt 4321 gaaacactaa aagctgaaaa tcaacctata agagatgtcc taaagcaact catattagtg 4381 ctttgttcag aagagaatat gcaaaaagcc cttgaattga aagcaaaata tgaatccgac 4441 atggttactg gtggctatgc agctttaata aatttatgct gtcgacatga taaagtagaa 4501 gatgccttga acttgaaaga agaatttgac cgcttagatt catctgctgt ccttgacacc 4561 ggcaagtatg taggccttgt aagagtattg gcaaagcatg gcaagctcca agatgctatt 4621 aacattctga aggagatgaa agagaaggat gttcttatca aagatacaac agccttgtcc 4681 tttttccaca tgctaaatgg cgcagcttta agaggtgaaa ttgaaacagt aaaacagttg 4741 catgaagcca tcgtgactct agggttagca gaaccatcca ccaacataag tttcccattg 4801 gtcactgtac acttggaaaa gggcgaccta tctactgctc ttgaggtcgc cattgactgc 4861 tatgaaaagt ataaagtatt accaaggatt catgatgtct tgtgtaaact ggtagagaaa 4921 ggcgagactg atctaattca gaaagcaatg gactttgtga gccaagaaca aggtgaaatg 4981 gtgatgctct atgatctctt ctttgccttc ctacaaacag gaaattacaa agaggccaag 5041 aagatcattg agactccagg gattagagct cgatctgcaa ggcttcagtg gttttgtgac 5101 agatgtgttg caaataatca ggttgaaact ctggaaaaat tagtggagct gacacagaag 5161 ctatttgaat gtgatagaga ccagatgtac tacaatctgc taaaactgta taaaataaac 5221 ggtgactggc aaagagctga tgcagtctgg aataaaatcc aagaagaaaa tgttattcct 5281 cgtgaaaaga cattaagatt attagcagaa atccttagag agggtaacca ggaagttccg 5341 tttgacgtac ctgagttgtg gtatgaagat gaaaaacatt ccctgaattc ttcgtcagcc 5401 tcaaccacag aacctgattt ccagaaagat atattgattg cctgccgatt gaaccaaaaa 5461 aaaggggcat atgatatttt cctgaatgca aaagagcaaa acattgtgtt taatgctgaa 5521 acctacagca atctcattaa attactgatg tcagaagatt attttacaca agcaatggaa 5581 gtgaaagcat tcgcggagac ccacatcaag ggcttcacac tgaacgatgc tgccaacagc 5641 cgcctcatca taacgcaagt taggcgggat tatttgaaag aggctgtgac aacactgaaa 5701 acagtattgg atcagcagca gaccccttct aggttagcag tgacccgtgt catccaggca 5761 ttggccatga agggtgatgt tgaaaacata gaagtagttc agaagatgtt aaatggactc 5821 gaagactcca ttggactttc aaaaatggtt ttcatcaata acattgcttt ggctcaaata 5881 aagaataata acatagatgc cgcaatagaa aacattgaaa atatgcttac ttcagagaat 5941 aaagtcattg aaccccaata cttcggcttg gcatacttat tcagaaaagt aatagaggag 6001 cagttggaac cagcagttga aaagataagc atcatggcgg agagattggc caatcagttt 6061 gcaatttata aacctgtcac tgattttttc cttcaacttg tggatgcagg caaggtggat 6121 gatgccagag ctctcctaca gagatgtggt gcaattgctg aacaaacccc gattttgttg 6181 ttgttcctcc ttaggaattc taggaaacaa ggaaaggcat caactgtgaa atctgtgtta 6241 gaattgattc ctgaattaaa tgaaaaggaa gaagcataca attccctcat gaaaagctat 6301 gtctcagaga aagatgtcac atctgctaaa gcactgtatg aacatttgac tgcaaagaat 6361 acaaaattgg atgatctgtt tctaaagcgt tacgcatctt tgctgaagta tgctggagag 6421 cctgtccctt tcattgaacc ccctgaaagc tttgaatttt atgcacagca gctaagaaaa 6481 ttgagggaaa actcttctgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 6541 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 6601 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 6661 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 6721 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 6781 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 6841 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 6901 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 6961 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 7021 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 7081 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 7141 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 7201 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 7261 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 7321 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 7381 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 7441 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 7501 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 7561 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 7621 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 7681 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 7741 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 7801 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 7861 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 7921 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 7981 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 8041 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 8101 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 8161 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 8221 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 8281 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 8341 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 8401 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 8461 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 8521 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 8581 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 8641 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 8701 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 8761 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 8821 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 8881 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 8941 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 9001 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 9061 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 9121 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 9181 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 9241 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 9301 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 9361 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 9421 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 9481 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 9541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 9661 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 9721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 9781 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 9841 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 9901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 9961 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 10021 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 10081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 10141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 10201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 10261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 10321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 10381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 10441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 10501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 10561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 10621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 10681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 10741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 10801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 10861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 10921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 10981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 11041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 11101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 11161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 11221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 11281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 11341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 11401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 11461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 11521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 11581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 11641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 11701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 11761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 11821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 11881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 11941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 12001 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 12061 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 12121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 12181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 12241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 12301 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 12361 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 12421 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???