pLV3-CMV-MAPK14-3×FLAG-P2A-EGFP plasmid

PVT110541

pLV3-CMV-MAPK14-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-MAPK14(human)-3×FLAG-P2A-EGFP
Alias: NM_001315.3;CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38;p38ALPHA; PRKM14; PRKM15; RK; SAPK2A
Gene lenght: 1080
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MAPK14-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-MAPK14-3×FLAG-P2A-EGFP It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-MAPK14-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc taccggactc agatctcgag gccaccatgt ctcaggagag gcccacgttc 2521 taccggcagg agctgaacaa gacaatctgg gaggtgcccg agcgttacca gaacctgtct 2581 ccagtgggct ctggcgccta tggctctgtg tgtgctgctt ttgacacaaa aacggggtta 2641 cgtgtggcag tgaagaagct ctccagacca tttcagtcca tcattcatgc gaaaagaacc 2701 tacagagaac tgcggttact taaacatatg aaacatgaaa atgtgattgg tctgttggac 2761 gtttttacac ctgcaaggtc tctggaggaa ttcaatgatg tgtatctggt gacccatctc 2821 atgggggcag atctgaacaa cattgtgaaa tgtcagaagc ttacagatga ccatgttcag 2881 ttccttatct accaaattct ccgaggtcta aagtatatac attcagctga cataattcac 2941 agggacctaa aacctagtaa tctagctgtg aatgaagact gtgagctgaa gattctggat 3001 tttggactgg ctcggcacac agatgatgaa atgacaggct acgtggccac taggtggtac 3061 agggctcctg agatcatgct gaactggatg cattacaacc agacagttga tatttggtca 3121 gtgggatgca taatggccga gctgttgact ggaagaacat tgtttcctgg tacagaccat 3181 attaaccagc ttcagcagat tatgcgtctg acaggaacac cccccgctta tctcattaac 3241 aggatgccaa gccatgaggc aagaaactat attcagtctt tgactcagat gccgaagatg 3301 aactttgcga atgtatttat tggtgccaat cccctggctg tcgacttgct ggagaagatg 3361 cttgtattgg actcagataa gagaattaca gcggcccaag cccttgcaca tgcctacttt 3421 gctcagtacc acgatcctga tgatgaacca gtggccgatc cttatgatca gtcctttgaa 3481 agcagggacc tccttataga tgagtggaaa agcctgacct atgatgaagt catcagcttt 3541 gtgccaccac cccttgacca agaagagatg gagtccgtcg acGACTACAA AGACCATGAC 3601 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGGG AAGCGGAGCC 3661 ACTAACTTCT CCCTGTTGAA ACAAGCAGGG GATGTCGAAG AGAATCCCGG GCCAgaattc 3721 GCCACCatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 3781 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3841 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 3901 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 3961 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 4021 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 4081 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 4141 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 4201 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 4261 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 4321 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 4381 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 4441 aagtaaGGAT CCaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 4501 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 4561 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 4621 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 4681 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 4741 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 4801 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 4861 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 4921 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 4981 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc 5041 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 5101 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 5161 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 5221 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 5281 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 5341 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 5401 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5521 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 5581 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 5641 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 5701 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 5761 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 5821 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 5881 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 5941 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 6241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 6481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 6541 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 6601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 6661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 6721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 6781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 6841 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 6901 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 6961 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7021 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7081 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7141 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7201 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7261 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7321 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7381 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7441 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7501 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7561 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7621 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7681 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 7741 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 7801 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 7861 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 7921 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 7981 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 8041 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 8101 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 8161 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 8221 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 8281 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 8341 cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.