pLV3-CMV-Mier2-3×FLAG-mCherry-Hyg plasmid

PVT107583

pLV3-CMV-Mier2-3×FLAG-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-Mier2(mouse)-3×FLAG-mCherry-Hyg
Alias: NM_001316699.2;2700087H15Rik; mi-er2; mKIAA1193
Gene lenght: 1623
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mier2-3×FLAG-mCherry-Hyg plasmid Description

pLV3-CMV-Mier2-3×FLAG-mCherry-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Mier2-3×FLAG-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcA AGCTTggatc cGCCACCatg gcggaggcct cctctctgga gaggcagagt 2341 cctcgtgtgg cctcctgcct tgtacacagc ctgtgtcctc gggagcccag cttgcagacc 2401 acggcagtgg tatccatggg ctctgccgac catcagttca accttgctga gctcctgacg 2461 cagaactaca acctccaaga agggtgtgct gaggccccgc agtgtcctga caagccggag 2521 gaggagctgg acaaggactt cattgaccag agcagtgaca tgcccctgga cgagctgctt 2581 gcactgtatg ggtacgagtc gtcagacccc atctctgagc aggagagtga aggcggtgac 2641 acggctccag ccctgccgga catgaccttg gacaaggagc agatagccaa ggacttgctt 2701 tctggggaag aagaggaaga aacacagtcc tccgctgatg acctcacccc gtctgtcacc 2761 tcccatgagg cctcggacct cttccataac cagagtgggt cccggttcct ggctggtgat 2821 aatggcccag gctcctcagc ctcctctgac actgaagagg acgcgcttcc tgccaacaag 2881 tgcaagaagg agatcatggt agggcctcaa ttccaagctg acctcaacat cctgcacttg 2941 aaccgacatt gtgacaagat ctatgaaaat gaagaccagc tgctgtggag ccccagtgta 3001 ctcccagagc gggaggtgga ggagttcctg tacagggcag tgaaacggag gtggcaggag 3061 atggctggcc cccagatccc agagggcgag gtggtgaagg acagtgagca ggcgctgtat 3121 gagctcgtga agtgtaattt caacgtggag gaggccctgc gcaggctgag gttcaacgta 3181 aaagtgatca gagatgggct ctgtgcctgg agcgaggagg agtgcaggaa ctttgaacat 3241 ggcttccgtg ttcatgggaa gaactttcat ctgattcagg ccaacaaggt acgcacgcgg 3301 tccgtgggcg agtgtgtgga gtattattac ctgtggaaga agtctgagcg ctacgactac 3361 tttgcccagc agacacggct gggccggagg aagtttgtct cctctggaac cacggatact 3421 gaacaggacc tagatgggct tgaccccgat ggccatgccc ggctgcattc tgaggcaggg 3481 gtgcctgtgg agcccctgaa tgtggacatt gaagctggtg gacttgacca gcctggcgtg 3541 ggctcggatg acctcccttc ctcagagcca ggaccgcgtc cattccagca gctggacgaa 3601 ccccctgctg tgccctcgct ccagcagccc actagcctgg ccgcctcagc tgaactccca 3661 ccggcggctg cagctgctcc agagccgggc actagcccca ggctgcctgt ggaccttgcc 3721 ctacccgagg agctgcccct tgtgtccagc cctgtggctc tgagcgagga cactgcagag 3781 cccatggctc cagcacaggt ggccttgtcg gtcactgaat ttggactcat tggcattggg 3841 gatgtgaatc ccttcctaac tggccaccca gcgtgcccaa cctccacact gcactcggag 3901 cccttgtcac agtgcaatgt gatgacctgt CTCGAGatgG ACTACAAAGA CCATGACGGT 3961 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGgc ggccgcGaag 4021 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 4081 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa 4141 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 4201 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 4261 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg 4321 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc 4381 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc 4441 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact 4501 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc 4561 tacgctagac gccaccATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA 4621 GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA 4681 GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA 4741 GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA 4801 GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG 4861 CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA 4921 CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC 4981 CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT 5041 GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG 5101 CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC 5161 CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT 5221 CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA 5281 CAAGGTCGAG GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA 5341 GGAGAACCCT GGACCTGTCG ACatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa 5401 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga 5461 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg 5521 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc 5581 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg 5641 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca 5701 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 5761 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 5821 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 5881 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 5941 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 6001 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 6061 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 6121 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca 6181 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 6241 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 6301 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 6361 cagcactcgt ccgagggcaa aggaatagTC TAGAaatcaa cctctggatt acaaaatttg 6421 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6481 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6541 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 6601 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 6661 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 6721 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 6781 gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg 6841 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6901 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 6961 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 7021 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 7081 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 7141 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 7201 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 7261 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 7321 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 7381 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 7441 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 7501 tatcccgccc ctaactccgc cATCCCGCCC TAACTCCGCc agttccgccc attctccgcc 7561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 7621 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 7681 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 8041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 8101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 8161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 8221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 8281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 8341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8521 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 9001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 9061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 9121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 9181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 9241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 9301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 9361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 9421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 9481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9841 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9901 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9961 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 10021 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 10081 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 10141 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 10201 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 10261 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.