pLV3-CMV-MSC-3×FLAG-CopGFP-Puro plasmid

PVT111432

pLV3-CMV-MSC-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MSC(human)-3×FLAG-CopGFP-Puro
Alias: NM_005098.4;ABF-1; ABF1; bHLHa22; MYOR
Gene lenght: 618
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MSC-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MSC-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MSC-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgt ccacgggctc ggtgagtgat 2341 ccggaggaga tggagcttcg ggggctgcag cgggagtacc cggtccccgc ctccaagagg 2401 ccgcccctcc gcggcgtaga gcgcagctac gcctcgccca gtgacaactc gtcggcagag 2461 gaggaggacc ccgacggcga ggaggagcgc tgcgctctgg gcacagccgg cagcgcggaa 2521 ggctgcaaga ggaagcggcc ccgtgtggct gggggcggcg gcgcaggtgg tagcgcgggc 2581 ggtggtggca agaagcccct cccggccaag ggctcagccg cagagtgcaa gcagtcgcag 2641 cggaacgcgg ccaacgcccg tgagcgtgcc cggatgcgcg tgctgagcaa agccttctcc 2701 aggctcaaga ccagcctgcc ctgggtgccc cccgacacta agctctccaa gctggacacg 2761 ctccggctgg cttccagtta catcgctcac ctgcggcagc tgttgcagga ggaccgctat 2821 gagaacggct acgtgcaccc agtgaacctg acatggccat tcgtggtctc gggaagaccg 2881 gactctgaca ccaaagaagt ttccgcagcc aacagactat gtggaaccac cgctggatcc 2941 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3001 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3061 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 3121 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 3181 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 3241 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 3301 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 3361 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 3421 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 3481 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 3541 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 3601 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 3661 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 3721 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 3781 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 3841 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 3901 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 3961 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4021 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4081 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4141 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4201 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 4261 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 4321 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 4381 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 4441 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 4501 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 4561 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 4621 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 4681 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 4741 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 4801 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 4861 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 4921 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 4981 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 5041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 5101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 5161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 5221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 5281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 5341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 5401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 5461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 5521 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 5581 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 5641 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 5701 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 5761 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 5821 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 5881 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 5941 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6061 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 6121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 6181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 6241 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 6301 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6361 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6421 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 6841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 6901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 7741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 7861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 7921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 7981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8041 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8401 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8461 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 8521 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 8581 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 8641 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 8701 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 8881 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.