pLV3-CMV-MT2A-FLAG-EF1-CopGFP-T2A-Puro plasmid

PVT107228

pLV3-CMV-MT2A-FLAG-EF1-CopGFP-T2A-Puro plasmid Information

Plasmid name: pLV3-CMV-MT2A(human)-FLAG-EF1-CopGFP-T2A-Puro
Alias: NM_005953.5;MT-2; MT-II; MT2
Gene lenght: 183
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MT2A-FLAG-EF1-CopGFP-T2A-Puro plasmid Description

pLV3-CMV-MT2A-FLAG-EF1-CopGFP-T2A-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-MT2A-FLAG-EF1-CopGFP-T2A-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggatcc caactgctcc tgcgccgccg gtgactcctg 2341 cacctgcgcc ggctcctgca aatgcaaaga gtgcaaatgc acctcctgca agaaaagctg 2401 ctgctcctgc tgccctgtgg gctgtgccaa gtgtgcccag ggctgcatct gcaaaggggc 2461 gtcggacaag tgcagctgct gcgccggatc cGATTACAAG GACGACGATG ACAAGtagcg 2521 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2581 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 2641 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2701 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2761 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 2821 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 2881 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 2941 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 3001 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 3061 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 3121 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 3181 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 3241 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 3301 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 3361 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 3421 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 3481 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 3541 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 3601 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 3661 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 3721 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 3781 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 3841 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac 3901 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac 3961 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 4021 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 4081 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 4141 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 4201 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 4261 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 4321 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 4381 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 4441 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca 4501 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4561 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 4621 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 4681 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 4741 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 4801 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 4861 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 4921 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 4981 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct 5041 tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc 5101 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 5161 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg 5221 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5281 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 5341 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 5401 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 5461 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 5581 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg 5641 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 5701 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca 5761 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 5821 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 5881 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 5941 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6001 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 6481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 6721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 6901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 6961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 7201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 7261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 7321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 7381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 7441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 7501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 7561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 7621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 7681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 7741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 7801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 7861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 7921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 7981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 8041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 8101 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 8161 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 8221 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 8281 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 8341 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.