pLV3-CMV-Myof-3×FLAG-CopGFP-Puro Plasmid
Catalog No.:
PVT49729
pLV3-CMV-Myof-3×FLAG-CopGFP-Puro PlasmidAlias:NM_001302140.1;2310004N10Rik; 2310051D19Rik;E030042N20Rik; Fer1l3Gene length:6183bp
Host:
mammalian cells,Lentivirus
Use(s):
Protein expressionFragment Type:
ORF
Fragmented species:
Mouse
Prokaryotic resistance:
Amp
Screening Markers:
Puro
Green
Promoter:
CMV
replicon:
pUC
Copy Number:
Competent cells:
Stbl3
Temperature:
37Degrees
Back Bones:
Forward primer:CMV-F
Reverse primer:
Induction method:
Sequence:
acgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtTtatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagcCTGATACGAACTCGgaattcgccaccatgcttcgagtgattgtggaatctgccactaacatccctaaaacaaaatttgggaagccggatcctatcgtttctgtcatttttaaggatgagaaaaagaaaacaaagaaagttgataatgaactcaaccctgtctggaatgagattttggaatttgacttgagggggataccactggactcttcatcctccctggtgattgttgtcaaagattttgagacaattggacaaaataaattaattggcacagcaactgtctccctgaaggacctgattggggaccagaacaggtctttgccctacaaacagacctccctgctaaatgaaaagggacaagacactggggccaccattgacctggtgatcggctataccccaccttcagctccacatccaaatgatccgagcgggaccagtgtgccaggcatgggagaagaagaggaagaagatcaaggagatgaagacagggtagatggcatagtcagaggccctgggcccaaggggccatctgggacagtgtcagaagctcagctggctcggaggatcaccaaagggaagagcagccgaaggatgctgtccaacaagccacaggatttccagatccgtgtcagagtgatcgagggccgtcagttatgcggcaacaacataaggcccgtggtgaaagttcacatctgtggtcagacgcaccgcacgagaatcaagagagggaacaatccgttttttgacgagttatttttctacaatgtgcacatcacaccctctgagctcatggatgagatcatcagtatccgggtttataattcccattctttgagggcagattgtctgatgggggaatttaagattgatgttggatttgtttatgatgaacctggccacgctgtcatgaggaaatggctccttctcaatgacccagaagacaccagctcaggcgcaaaaggttacatgaaagtcagcatgtttgtcctgggaaccggagacgaaccccctcctgagaaacgagaccgtgacaatgacagtgatgacgtggagagcaacttgttacttccggcaggcattgccctccggtgggtgaccttcatgctgaagatctaccgagccgaggacatcccccagatggatgatgccttctcacaaacagtgaaggaaatatttggtggcaacgcagataaaaaaaatcttgtggatccttttgtagaagtctcctttgctgggaaaaaggtgtgcacaaatataattgagcggaatgcaaaccctgaatggaatcaagttgtcaaccttcagatcaagtttccctccatgtgtgaaaaaataaaactaacagtatatgactgggaccggcttactaaaaatgatgtagttgggaccacgtacctgtacctctctaaaattgctgcctctgggggagaagtggaagatttctcatcctcgggagctggggctgcatcatatacagcaactacaggagagacagaggtggggtttgtcccaacattcggaccttgttatttgaacctttacggaagccccagagagtacacaggatttccagatccctatgatgagctcaactcaggaaagggagaaggggtggcctacagaggcaggatcttcgtggaactgaacacgttccttgaaaagaaaccaccagaaaagaagctggagcccatttccagtgatgaccttctggttgttgagaagtaccagcgaaggcggaagtatagcctgtccgccgtgttccattcagccaccatgttgcaagatgttggtgaggccattcagtttgaagtcagcattgggaactatggcaacaagtttgatgcaacctgtaaacctttggcatcaaccactcaatacagccgtgcagtatttgacgggaactactattactacttaccttgggcccacacgaagcccgtggtcaccctgacttcctactgggaggatattagtcaccgcctggatgcagtgaacactctcctagtcatggcagaacggctgcaatcaaacatagaggctgtaaaatcagggatccagggtaaaattcctgcaaaccagttggcggaggtgtggctgaagctgatagatgaagttatagaagacacgagatacaccttgcctgtcacagaaggaaaagccaacgtcacggttctggatactcagatccgaaagttgcggtccaggtttctctcccagatacacgaggcagctctgaggatgagatctgaagccacggatgtgaaatccaccttgttggaaatcgaggagtggcttgataaactgatgcagcttactgaagagcctcagaacagcatgcctgacatcatcatctggatgatccgtggagagaaaagactggcttatgcacgaatccctgcccaccaagtcttgtactccaccagcggtgggaacgcctctggaaaatactgtgggaaaacccaaaccatccttctgaagtatccacaagaaaaaaccaatgggcccaaagtgcctgtggagctgcgcgtgaacatctggctgggtttaagtgctgtggaaaagaagttcaatagcttcgcggaaggcaccttcaccgtctttgctgaaatgtatgaaaaccaagcccttgtgtttgggaagtggggcacctctggactggtgggacgccataaattctctgatgtcacaggaaaaataaaactcaagagagaattctttttacctcccaaaggctgggagtgggaaggagactgggtggttgaccctgaacgaagcctcctgactgaggctgacgcaggtcacaccgagttcactgatgaagtctaccagaatgagaatcgctaccccggaggcgagtggaagcaggcagaggacacctatacggatgcgaatggagataaggcggcatcacccagtgagatgacctgccctccaggttgggagtgggaggatgacgcatggatatatgacataaatcgagcagtggatgagaaaggctgggagtatgggattaccattcctcctgataataagcccaaatcctgggttgcagcagaaaagatgtatcacactcacaggcggcggagactggtccggaaacgcaagaaagatctgacacagacggcttctagtaccgcaagggccatggaagaacttgaagaccgggagggctgggaatatgcttcgttaatcggctggaaattccactggaaacagcgcagttcagacacctttcgccgcaggcgctggaggaggaaaatggccccttctgagacacacggtgccgctgccatctttaaactcgaaggtgcccttggggcagacaccacagaggatggagaagagaagggcccggagaagcagaagcacagcgccaccactgtgtttggagcaaacacgcccattgtttcctgtaatttcgacagagtctacatctaccacctgcgctgctacatttatcaagccaggaacctcatggctttggacaaagacagcttttcagatccatacgcccatgtctccttcctccatcgaagcaaaaccactgagatcatccactccaccctgaatcccacctgggaccaaaccattatattcgatgaggttgaaatctttggggaaccccaaacagttctacagaacccacccaacgttaccatagaactttttgacaatgaccaagtgggcaaagatgaatttttaggtcgaagcatttgctctcccttggtgaaactgaactcagaaacggacatcacacctaagcttctctggcatcctgttatgaatggagacaaggcctgtggggatgtcctcgtaacagcagagttgattctgaggaacaaggatggctccaaccttcccatcctcccctctcaaagagcaccaaacttatacatggtccctcaggggatcaggcctgtggttcagctcactgccattgagatcctagcttggggcttaagaaatatgaaaaactaccagatggcttctgtcacatcacccagcctggtggtggaatgcggaggagagagagtagaatcggtggtgatcaaaagccttaagaagacacccaacttcccaagttctgttctcttcatgaaagtgttcttgcccaaggaagaattgtacatgcctccgctggtgatcaaggtcattgatcacaggcagtttgggcggaagcctgtggttggccaatgcaccattgaccacctggaccgctttcggtgtgatccatatgcaggaaaggaggatattgtaccacaactaaaagcctctctgatgtctgcacctccatgccgggaagtcgttattgaaatagaagacaccaaaccattactggcttctaagctgtcggaaaaggaggaagaaatcgttgactggtggagtaaattttatgcttcctctggagagcatgaaaaatgtggacagtatattcagaaaggctattccaaactcaagatctatgactgtgaactggaggatgtagccgactttgaaggcctgacagatttctcagataccttcaagctgtacagaggcaagtccgatgagaatgaagatccatctgtggtaggagagtttaaaggctccttccgaatctaccccttgccggatgaccccagtgtgccagcccctcccaggcagtttcgggaactgcctgacagtgtcccacaggaatgcactgttaggatttacattgttcaaggcttacagctccagcctcaggacaacaatggcctgtgtgatccttacataaaaataaccttgggcaagaaagtaattgaagaccgagaccactacattcccaacactctcaacccagtgtttggcagaatgtacgaactgagctgctacttgcctcaagagaaagacctgaagatctctgtctatgactacgataccttcacccgagatgagaaggtgggagagacaaccattgacctggagaaccgcttcctttcccggttcgggtctcactgcggaatccctgagcagtactgtgtttccggagtaaatacctggcgagatcagctgagaccaacgcagttgcttcaaaacgttgcaagattcaaaggcttcccgccaccagttctttctgaagacggaagcagaatccgatacggaggtcgagactaccatttggatgagtttgaggccaacaaaatcctgcaccagcaccttggagcccccgaagagcgtcttgccctccatattctcaggactcaggggctggtccctgagcacgtggagacaaggactttgcacagcaccttccagcccaatatttcccaggggaaacttcagatgtgggtggatgtcttccccaagagtttaggaccaccaggcccccctttcaacatcacacccaggaaagccaagaagtactacctgcgtgtgatcatctggaataccaaggacgttatcttggatgagaagagcatcactggggaggacatgagtgacatctacgtcaaaggctggatttccggcagcgaagaaaacaaacagaaaacagatgtccactacaggtccttggatggtgaaggcaattttaactggagatttgttttcccatttgactacctccctgctgagcagctctgtatagtcgctaaaaaagagcatttctggagtattgatcaaacagaatttcgagtccctcctcggctgatcattcagatatgggacaatgacaagttttccctggacgactacttgggttttctggaacttgacttacatcgtacgatcatcccagccaagacatcagagaaatgcagtttggacatgattccagacctcaaagccatggaccctcttaaagctaagactgcctcactctttgaacagaggtccatgaaaggatggtggccgtgctacgcagacaaagatggcacccgggtgatggctgggaaagtggagatgacactggaggtgctcaacgagagggaggccgacgagaggccagccgggaaggggcggagtgaacccaacatgaaccccaagcttgacccaccaaaccgaccggaaacctccttcctctggttcacgaacccttgcaagaccatgcgcttcatcgtgtggcgccggttcaagtgggtgatcatcggcctgcttctgctgctcatcctgttgctgttcgtggctgtgctcctgtactctcttccgaactatttatcaatgaagattgtgaggccgaatgcaggatccGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGtagcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaATCATCGTCCTTTCCTtggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctccgccttcgccctcagacgagtcggatctccctttggGccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaGataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctaTCCCGCCCCTAACTCCGCCCAtcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagaccaaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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