pLV3-CMV-NACA-3×FLAG-CopGFP-Puro plasmid

PVT92945

pLV3-CMV-NACA-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NACA(human)-3×FLAG-CopGFP-Puro
Alias: NM_001113203.3;HSD48; NAC-alpha; NACA1; skNAC
Gene lenght: 2778bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NACA-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NACA-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-NACA-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc ccggcgaagc cacagaaacc 2341 gtccctgcta cagagcagga gttgccgcag ccccaggctg agacagctgt gctacctatg 2401 tcttcagcct tgagtgtcac tgctgcctta gggcagcctg gacctaccct cccccctcct 2461 tgctctcctg ccccacaaca gtgccctctc tcagctgcta accaggcttc cccattccct 2521 tccccctcta ctattgcctc gaccccttta gaagttcctt ttccccagtc atcctctgga 2581 acagccctac ctttgggaac tgcccctgaa gccccaacct tcctaccaaa cctaataggg 2641 cctcccatct ccccagctgc cttagctcta gcctctccca tgatagctcc aactctgaaa 2701 gggacccctt cctcttcagc tcccttagct ctggttgccc tggctcccca ctcagttcag 2761 aagagttctg cttttccacc taaccttctt acttcacctc cttcagtggc tgtagctgag 2821 tcagggtcag tgataactct gtcagctccc attgctccct cagaaccaaa gactaatctt 2881 aataaagttc cctctgaggt agtccctaat ccaaaaggca cccccagccc tccatgtata 2941 gtcagtactg ttccttacca ctgtgtgact cccatggcct ctattcaatc tggagtggcc 3001 tcccttcctc agacaacacc cacaactacc ctagccatcg cttcccctca agtcaaagat 3061 accaccattt cctcagttct gatttctcca caaaacccag gaagcctcag cctgaagggg 3121 cctgttagtc cacctgctgc cttatctctt tcaactcagt ctcttcctgt ggtgacctct 3181 tctcaaaaga ctgcgggtcc caacaccccc ccagattttc ccatttctct gggctctcat 3241 cttgcacctt tacatcagag ttcttttggt tctgtccaac ttttaggtca aacaggtcct 3301 agtgctttgt cagaccctac agtgaagacc atttctgtag atcattcttc cacaggggcc 3361 tcttatcctt ctcagagatc tgtaattcct ccccttcctt ccagaaatga ggtagttcct 3421 gctactgtgg ctgcctttcc agtggtggct ccatctgttg acaaaggtcc ctctaccatc 3481 tctagcataa cctgcagccc ttctggctcc ttaaatgtag ctacctcttt ttcattatct 3541 cctacaacct ctctcattct caaaagctct cctaatgcca cttatcatta tcctttagtg 3601 gcccaaatgc ccgtttcttc tgttggaacc accccacttg tggtgactaa cccctgtaca 3661 attgctgcag cacctactac tacctttgag gtagctactt gtgtttctcc tccaatgtca 3721 tcaggtccca taagtaacat agaaccaact tcccctgctg ccttggttat ggcacctgtg 3781 gctcccaaag agccttctac tcaagtagca accactctga ggataccagt ctctcctcct 3841 ctgccagacc ctgaagacct caaaaatctc cccagttcag tattggttaa atttccaaca 3901 caaaaagacc tccaaactgt acctgcctct cttgaaggag cccctttctc tccagcccaa 3961 gcaggactca ccaccaagaa agaccctact gtattaccgt tagtccaggc agcccctaaa 4021 aattcccctt ctttccaaag tacatcctct tctccagaga tacctctttc tcctgaagcc 4081 accctagcaa agaaaagcct tggggagcct ctccctatag tggctgcatt tcctttggaa 4141 agtgctgacc ctgccggggt ggctcccaca actgccaaag cagctgcctt tgagaaggtc 4201 cttcctaaac ctgaatcagc atctgtctct gcagcaccct ccccaccagt ctctctgcct 4261 cttgctccct ccccagttcc cactctgcct cctaaacagc aatttctgcc gtcctctcct 4321 gggctggtgt tggaatcacc ctctaaaccc cttgcccctg ctgatgagga tgagctgctg 4381 cctctgattc ccccggaacc aatctctggg ggagtgcctt tccagtcggt cctcgtcaac 4441 atgcccaccc ctaaatctgc tggaatccct gtcccaaccc cctctgccaa gcaacctgtt 4501 acgaagaaca acaaggggtc tggaacagaa tctgacagtg atgaatcagt accagagctt 4561 gaagaacagg attccaccca ggcaaccaca caacaagccc agctggcggc agcagctgaa 4621 attgatgaag aaccagtcag taaagcaaaa cagagtcgga gtgaaaagaa ggcacggaag 4681 gctatgtcca aactgggtct tcggcaggtt acaggagtta ctagagtcac tatccggaaa 4741 tctaagaata tcctctttgt catcacaaaa ccagatgtct acaagagccc tgcttcagat 4801 acttacatag tttttgggga agccaagatc gaagatttat cccagcaagc acaactagca 4861 gctgctgaga aattcaaagt tcaaggtgaa gctgtctcaa acattcaaga aaacacacag 4921 actccaactg tacaagagga gagtgaagag gaagaggtcg atgaaacagg tgtagaagtt 4981 aaggacattg aattggtcat gtcacaagca aatgtgtcga gagcaaaggc agtccgagcc 5041 ctgaagaaca acagtaatga tattgtaaat gcgattatgg aattaacaat gggatccGAC 5101 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 5161 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 5221 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 5281 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 5341 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 5401 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 5461 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 5521 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 5581 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 5641 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 5701 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 5761 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 5821 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 5881 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 5941 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 6001 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 6061 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 6121 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 6181 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 6241 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 6301 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 6361 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 6421 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 6481 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 6541 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 6601 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 6661 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 6721 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 6781 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 6841 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 6901 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 6961 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 7021 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 7081 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 7141 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 7201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 7261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 7321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 7381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 7441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 7501 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 7561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 7621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 7681 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 7741 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 7801 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 7861 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 7921 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 7981 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 8041 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 8101 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 8161 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 8221 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 8281 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 8341 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 8401 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 8461 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 8521 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 8581 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 8641 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 8701 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 8761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 8821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 9001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 9061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 9121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 9181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 9241 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 9301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 9361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 9421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 9481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 9601 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 9661 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 9721 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 9781 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 9841 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 9901 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 9961 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 10021 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 10081 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 10141 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 10201 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 10261 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 10321 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 10381 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 10441 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 10501 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 10561 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 10621 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 10681 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 10741 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 10801 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 10861 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 10921 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 10981 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 11041 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???