pLV3-CMV-NFIL3-3×FLAG-CopGFP-Puro plasmid

PVT102822

pLV3-CMV-NFIL3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NFIL3(human)-3×FLAG-CopGFP-Puro
Alias: NM_005384.3;E4BP4; IL3BP1; NF-IL3A; NFIL3A;
Gene lenght: 1386
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NFIL3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NFIL3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NFIL3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc agctgagaaa aatgcagacc 2341 gtcaaaaagg agcaggcgtc tcttgatgcc agtagcaatg tggacaagat gatggtcctt 2401 aattctgctt taacggaagt gtcagaagac tccacaacag gtgaggagct gcttctcagt 2461 gaaggaagtg tggggaagaa caaatcttct gcatgtcgga ggaaacggga attcattcct 2521 gatgaaaaga aagatgctat gtattgggaa aaaaggcgga aaaataatga agctgccaaa 2581 agatctcgtg agaagcgtcg actgaatgac ctggttttag agaacaaact aattgcactg 2641 ggagaagaaa acgccacttt aaaagctgag ctgctttcac taaaattaaa gtttggttta 2701 attagctcca cagcatatgc tcaagagatt cagaaactca gtaattctac agctgtgtac 2761 tttcaagatt accagacttc caaatccaat gtgagttcat ttgtggacga gcacgaaccc 2821 tcgatggtgt caagtagttg tatttctgtc attaaacact ctccacaaag ctcgctgtcc 2881 gatgtttcag aagtgtcctc agtagaacac acgcaggaga gctctgtgca gggaagctgc 2941 agaagtcctg aaaacaagtt ccagattatc aagcaagagc cgatggaatt agagagctac 3001 acaagggagc caagagatga ccgaggctct tacacagcgt ccatctatca aaactatatg 3061 gggaattctt tctctgggta ctcacactct cccccactac tgcaagtcaa ccgatcctcc 3121 agcaactccc cgagaacgtc ggaaactgat gatggtgtgg taggaaagtc atctgatgga 3181 gaagacgagc aacaggtccc caagggcccc atccattctc cagttgaact caagcatgtg 3241 catgcaactg tggttaaagt tccagaagtg aattcctctg ccttgccaca caagctccgg 3301 atcaaagcca aagccatgca gatcaaagta gaagcctttg ataatgaatt tgaggccacg 3361 caaaaacttt cctcacctat tgacatgaca tctaaaagac atttcgaact cgaaaagcat 3421 agtgccccaa gtatggtaca ttcttctctt actcctttct cagtgcaagt gactaacatt 3481 caagattggt ctctcaaatc ggagcactgg catcaaaaag aactgagtgg caaaactcag 3541 aatagtttca aaactggagt tgttgaaatg aaagacagtg gctacaaagt ttctgaccca 3601 gagaacttgt atttgaagca ggggatagca aacttatctg cagaggttgt ctcactcaag 3661 agacttatag ccacacaacc aatctctgct tcagactctg ggggatccGA CTACAAAGAC 3721 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 3781 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 3841 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3901 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3961 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 4021 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 4081 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 4141 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 4201 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 4261 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 4321 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 4381 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 4441 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 4501 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 4561 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 4621 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 4681 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 4741 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 4801 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 4861 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 4921 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 4981 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 5041 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 5101 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 5161 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 5221 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 5281 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5341 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5401 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5461 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 5521 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 5581 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5641 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5701 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 5761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 6001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 6061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 6121 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 6181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 6301 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 6361 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 6421 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 6481 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 6541 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6601 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 6661 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 6721 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 6781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 6841 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 6901 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 6961 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 7021 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7861 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 9181 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 9241 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 9301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 9361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 9421 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 9481 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 9541 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 9601 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 9661 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???