pLV3-CMV-ONECUT2-3×FLAG-CopGFP-Puro plasmid

PVT97979

pLV3-CMV-ONECUT2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ONECUT2(human-gc-opt)-3×FLAG-CopGFP-Puro
Alias: NM_004852.3;OC-2; OC2
Gene lenght: 1512bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ONECUT2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ONECUT2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ONECUT2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGA AGGCTGCCTA CACCGCCTAT 2341 CGATGCCTCA CCAAAGACCT AGAAGGCTGC GCCATGAACC CGGAGCTGAC AATGGAAAGT 2401 CTGGGCACTT TGCACGGACC CGCAGGTGGA GGGAGCGGAG GGGGGGGGGG GGGAGGAGGT 2461 GGAGGTGGAG GGGGAGGCCC TGGGCACGAA CAGGAACTGC TGGCATCCCC GTCCCCTCAC 2521 CACGCAGGCA GAGGAGCAGC GGGAAGCTTG CGCGGCCCAC CACCTCCTCC AACCGCGCAC 2581 CAGGAGCTGG GCACGGCGGC AGCGGCGGCA GCGGCGGCGT CGCGCTCGGC CATGGTCACC 2641 AGCATGGCCT CGATCCTGGA CGGCGGCGAC TACCGGCCCG AGCTCTCCAT CCCGCTGCAC 2701 CACGCCATGA GCATGTCCTG CGACTCGTCT CCGCCTGGCA TGGGCATGAG CAACACCTAC 2761 ACCACGCTGA CACCGCTCCA GCCGCTGCCA CCCATCTCCA CCGTGTCTGA CAAGTTCCAC 2821 CACCCTCACC CGCACCACCA TCCGCACCAC CACCACCACC ACCACCACCA GCGCCTGTCC 2881 GGCAACGTCA GCGGCAGCTT CACCCTCATG CGCGACGAGC GCGGGCTCCC GGCCATGAAC 2941 AACCTCTACA GTCCCTACAA GGAGATGCCC GGCATGAGCC AGAGCCTGTC CCCGCTGGCC 3001 GCCACGCCGC TGGGCAACGG GCTAGGCGGC CTCCACAACG CGCAGCAGAG TCTGCCCAAC 3061 TACGGTCCGC CGGGCCACGA CAAAATGCTC AGCCCCAACT TCGACGCGCA CCACACTGCC 3121 ATGCTGACCC GCGGTGAGCA ACACCTGTCC CGCGGCCTGG GCACCCCACC TGCGGCCATG 3181 ATGTCGCACC TGAACGGCCT GCACCACCCG GGCCACACTC AGTCTCACGG GCCGGTGCTG 3241 GCACCCAGTC GCGAGCGGCC ACCCTCGTCC TCATCGGGCT CGCAGGTGGC CACGTCGGGC 3301 CAGCTGGAAG AAATCAACAC CAAAGAGGTG GCCCAGCGCA TCACAGCGGA GCTGAAGCGC 3361 TACAGTATCC CCCAGGCGAT CTTTGCGCAG AGGGTGCTGT GCCGGTCTCA GGGGACTCTC 3421 TCCGACCTGC TCCGGAATCC AAAACCGTGG AGTAAACTCA AATCTGGCAG GGAGACCTTC 3481 CGCAGGATGT GGAAGTGGCT TCAGGAGCCC GAGTTCCAGC GCATGTCCGC CTTACGCCTG 3541 GCAGCGTGCA AACGCAAAGA GCAAGAACCA AACAAAGACA GGAACAATTC CCAGAAGAAG 3601 TCCCGCCTGG TGTTCACTGA CCTCCAACGC CGAACACTCT TCGCCATCTT CAAGGAGAAC 3661 AAACGCCCGT CAAAGGAGAT GCAGATCACC ATTTCCCAGC AGCTGGGCCT GGAGCTCACA 3721 ACCGTCAGCA ACTTCTTCAT GAACGCCCGG CGCCGCAGCC TGGAGAAGTG GCAAGACGAT 3781 CTGAGCACAG GGGGCTCCTC GTCCACCTCC AGCACGTGTA CCAAAGCAgg atccGACTAC 3841 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3901 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3961 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4021 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4081 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4141 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4201 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4261 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4321 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4381 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4441 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4501 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4561 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4621 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4681 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4741 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4801 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4861 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4921 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4981 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5041 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5101 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5161 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5221 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5281 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5341 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5401 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5461 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5521 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5581 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5641 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5701 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5761 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5821 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5881 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5941 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6001 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6061 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6121 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6181 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6241 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 6301 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6361 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 6421 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 6481 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 6541 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 6601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 6661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 6721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 6781 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 6841 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6961 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 7021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7081 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7141 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 7201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7321 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7381 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7981 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9301 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9361 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9541 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9601 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9661 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9721 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9781 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???