pLV3-CMV-OXCT1-Fluc-Puro plasmid

PVT95537

pLV3-CMV-OXCT1-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-OXCT1(human)-Fluc-Puro
Alias: NM_001364299.2;OXCT; SCOT
Gene lenght: 1584bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-OXCT1-Fluc-Puro plasmid Description

pLV3-CMV-OXCT1-Fluc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-OXCT1-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catggcggct ctcaaactcc tctcctccgg gcttcggctc 2341 tgcgcctctg cccgcggatc tggggcaacc tggtacaagg gatgtgtttg ttccttttcc 2401 accagtgctc atcgccatac caagttttat acagatccag tagaagctgt aaaagacatc 2461 cctgatggtg ccacggtttt ggttggtggt tttgggctat gtggaattcc agagaatctt 2521 atagatgctt tactgaaaac tggagtaaaa ggactaactg cagtcagcaa caatgcaggg 2581 gttgacaatt ttggtttggg gcttttgctt cggtccaagc agataaaacg catggtctct 2641 tcatatgtgg gagaaaatgc agaatttgaa cgacagtact tatctggtga attagaagtg 2701 gagctgacac cacagttttt cctcaggcta agagagggca cacttgcaga gaggatccgt 2761 gcaggcgggg ctggagttcc tgcattttac accccaacag ggtatgggac cctggtacaa 2821 gaaggaggat cgcccatcaa atacaacaaa gatggcagtg ttgccattgc cagtaagcca 2881 agagaggtga gggagttcaa tggtcagcac tttattttgg aggaagcaat tacaggggat 2941 tttgctttgg tgaaagcctg gaaggcggac cgagcaggaa acgtgatttt caggaaaagt 3001 gcaaggaatt tcaacttgcc aatgtgcaaa gctgcagaaa ccacagtggt agaggttgaa 3061 gaaattgtgg atattggagc atttgctcca gaagacatcc atattcctca gatttatgta 3121 catcgcctta taaagggaga aaaatatgag aaaagaattg agcgtttatc aatccggaaa 3181 gagggagatg gggaagccaa atctgctaaa cctggagatg acgtaaggga acgaatcatc 3241 aagagggccg ctcttgagtt tgaggatggc atgtatgcta atttgggcat aggaatccct 3301 ctcctggcca gcaattttat cagcccaaat ataactgttc atcttcaaag tgaaaatgga 3361 gttctgggtt tgggtccata tccacgacaa catgaagctg atgcagatct catcaatgca 3421 ggcaaggaaa cagttactat tcttccagga gcctcttttt tctccagcga tgaatcattt 3481 gcaatgatta gaggtggaca cgtcgatctg acaatgctag gagcgatgca ggtttccaaa 3541 tatggtgacc tggctaactg gatgatacct gggaagatgg tgaaaggaat gggaggtgct 3601 atggatttag tgtccagtgc gaaaaccaaa gtggtggtca ccatggagca ttctgcaaag 3661 ggaaatgcac ataaaatcat ggagaaatgt acattaccat tgactggaaa gcaatgtgtc 3721 aaccgcatta ttactgaaaa ggctgtgttt gatgtggaca agaagaaagg gttgactctg 3781 attgagctct gggaaggcct gacagtggat gacgtacaga agagtactgg gtgtgatttt 3841 gcagtttcac caaaactcat gccaatgcag cagatcgcaa attgaggatc cgcggccgca 3901 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 3961 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 4021 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 4081 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 4141 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 4201 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4261 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 4321 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 4381 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 4441 cctacgctag acgccaccat ggagagcgac gagagcggcc tgatggaaga cgccaaaaac 4501 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg 4561 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat 4621 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct 4681 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt 4741 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac 4801 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg 4861 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc 4921 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg 4981 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc 5041 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa 5101 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt 5161 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt 5221 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat 5281 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg 5341 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta 5401 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg 5461 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca 5521 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt 5581 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa 5641 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa 5701 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg 5761 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc 5821 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac 5881 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt 5941 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta 6001 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt 6061 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga 6121 aagatcgccg tgagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 6181 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 6241 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 6301 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 6361 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 6421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 6481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 6541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 6601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 6661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 6721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 6781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 6841 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 6901 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 6961 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 7021 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 7081 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 7141 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 7201 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 7261 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 7321 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 7381 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc 7441 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 7501 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt 7561 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 7741 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 7801 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 7861 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7921 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 7981 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 8041 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 8101 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt 8161 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 8221 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 8281 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 8341 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 8401 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 8461 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 8521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 8581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 8641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 8701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 8761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 8821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 8881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 8941 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 9001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 9181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 9241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 9361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 9421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 9481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 9541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 9601 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 9661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 9721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 9781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 9841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 9901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 9961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 10021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 10081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 10141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 10201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 10261 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 10321 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 10381 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 10441 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 10501 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 10561 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 10621 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 10681 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 10741 cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???