pLV3-CMV-PCBP4-3×FLAG-CopGFP-Puro plasmid

PVT107052

pLV3-CMV-PCBP4-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PCBP4(human)-3×FLAG-CopGFP-Puro
Alias: NM_001174100.2;CBP; LIP4; MCG10
Gene lenght: 1209
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PCBP4-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PCBP4-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-PCBP4-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga gcggctcgga cgggggactg 2341 gaggaggagc cagagctcag catcaccctc acgctgcgga tgctgatgca cgggaaggaa 2401 gtgggcagca tcatcgggaa gaagggcgag actgtaaagc gaatccggga gcagagcagt 2461 gcccggatca ccatctccga gggctcctgc cctgaacgca tcaccaccat caccgggtct 2521 acagcagctg tcttccatgc agtctccatg attgctttca aactggatga ggacctttgt 2581 gctgctcctg caaatggtgg aaatgtctcc aggcctccag tgaccctgcg ccttgtcatc 2641 cctgccagtc agtgtggctc actgattggg aaggctggca ccaagatcaa ggagatccga 2701 gagactacgg gtgcccaggt acaggtggca ggggacctgc tccccaactc cacagagcga 2761 gctgttacgg tatctggggt gcctgatgcc atcatcctgt gtgtgcgcca gatctgcgct 2821 gttatcctgg agtccccacc caaaggagcc actatcccct accatccgag cctctcccta 2881 ggtactgttc ttctctctgc caaccagggc ttctctgtcc agggtcagta tggggctgtg 2941 accccagctg aggtcaccaa gctccagcag ctctcaagcc atgcggtccc ctttgccaca 3001 cccagcgtgg tgccaggact ggatcccggc acacagacca gctcacagga gttcttggtt 3061 cccaacgatt tgattggctg tgtgatcggg cgccagggca gcaagatcag cgagatccgg 3121 cagatgtcag gggcacatat caagatcggg aaccaagcag agggcgctgg ggagcggcat 3181 gtcaccatca ctggctctcc ggtctccatc gccctggccc agtacctcat cactgcctgt 3241 ctagagacgg ccaagtctac ctctgggggg acgcccagct cggcccccgc agacctgcct 3301 gcccccttct cgccacccct gacggccctg cccacagctc cccctggcct gctgggcaca 3361 ccctatgcca tctccctctc caacttcatc ggcctcaagc ccatgccctt cttggcttta 3421 ccacctgctt ccccagggcc gccgccgggc ttggcggcct acactgccaa gatggcagca 3481 gctaatggga gcaagaaggc tgagcggcag aaattctccc cctacggatc cGACTACAAA 3541 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 3601 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3661 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3721 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3781 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3841 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 3901 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 3961 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 4021 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 4081 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4141 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 4201 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4261 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4321 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4381 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 4441 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4501 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4561 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4621 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4681 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 4741 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 4801 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 4861 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 4921 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 4981 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 5041 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 5101 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5161 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5221 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5281 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5341 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5401 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5461 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5521 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5581 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5641 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5701 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5761 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 5821 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 5881 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 5941 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 6001 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 6061 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 6121 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 6181 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6241 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 6301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6481 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6541 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6601 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6661 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 6721 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6781 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6841 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 6901 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6961 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7021 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7081 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7141 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7681 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9001 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9061 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9301 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9421 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9481 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???