pLV3-CMV-Pdgfrb-3×HA-mCherry-Puro plasmid

PVT107791

pLV3-CMV-Pdgfrb-3×HA-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-Pdgfrb(mouse)-3×HA-mCherry-Puro
Alias: NM_008809.2;CD140b; Pdgfr; PDGFR-1
Gene lenght: 3294
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pdgfrb-3×HA-mCherry-Puro plasmid Description

pLV3-CMV-Pdgfrb-3×HA-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Pdgfrb-3×HA-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggggct tccaggagtg ataccagctt tagtcctcag 2341 aggccagttg ttgctgtccg tgttatggct cctgggaccg cagacctccc ggggcctagt 2401 catcacgccc cctgggccag agtttgttct caacatctcc agcacctttg ttctgacctg 2461 ctcgggctca gctccggtga tgtgggaaca gatgtcccag gtgccctggc aagaagcggc 2521 catgaatcag gacggcacct tctccagtgt gctgacactg accaatgtca ctgggggaga 2581 cactggggaa tacttttgtg tctacaacaa ctcactaggg ccggagctca gtgagaggaa 2641 gcgtatctat atctttgtgc cagatcccac catgggcttc ctccctatgg actccgagga 2701 cctgttcatt tttgtcacgg atgtcactga gacgacaatt ccgtgccgag tgacagaccc 2761 ccagctggag gtgacgctac atgagaagaa agtggatatc cccctacacg taccctacga 2821 ccaccagcga ggtttcactg gtacttttga ggacaagact tacatttgca aaaccaccat 2881 tggggacagg gaagtggact ccgatactta ctacgtctac agcctccagg tgtcatccat 2941 caacgtctct gtgaatgccg tgcagactgt ggtccgccag ggcgagagca tcaccatccg 3001 gtgcattgtg atgggcaatg atgtggtgaa cttccaatgg acgtaccccc gcatgaagag 3061 tgggcggctg gtggagccag tgacagacta cctctttgga gtgccctccc gcattggctc 3121 catcctgcat atccccacgg ctgagctgag tgattcgggc acctatactt gcaacgtgtc 3181 agtgagtgtg aacgaccatg gcgatgagaa agccatcaac atctctgtga tcgagaatgg 3241 ctacgtgcgg ctgctggaga cactgggaga tgtagaaatt gctgagctgc accggagtcg 3301 gacgctgcgg gtggtgttcg aggcttatcc gatgccttct gtcctgtggc tcaaggacaa 3361 ccgtaccttg ggtgactccg gcgctggcga gttagttttg tctactcgca acatgtctga 3421 gacccggtac gtgtcagaac tgatcctggt acgtgtgaag gtgtcagaag cgggctacta 3481 tactatgcga gccttccacg aggacgatga ggtccagctc tccttcaagc tgcaggtcaa 3541 tgtccccgtc cgtgtgctgg agctgagtga gagtcaccct gccaatgggg agcagacaat 3601 ccgctgtcgt ggccggggca tgcctcagcc aaatgtcacc tggtctacct gcagagacct 3661 caaaaggtgt ccacgaaaac tgtcacccac acccttgggg aatagttcca aggaggagag 3721 ccagctagaa acgaatgtga ctttctggga ggaagatcag gaatacgagg tggtgagcac 3781 actgcgcctg cgccacgtgg atcagccact gtccgtacgc tgcatgctgc agaactccat 3841 gggtggagat tcgcaggagg tcaccgtggt cccacattcc ttgcccttca aagtggtggt 3901 gatctcagcc atcctggcct tagtggtcct taccgtcatc tctctcatca tcctcatcat 3961 gctgtggcag aagaagccac gctatgagat ccgatggaag gtcattgagt ctgtgagctc 4021 tgacggtcat gagtacatct acgtggaccc tgtgcagttg ccttacgact ccacctggga 4081 gctgccacgg gaccagcttg ttctgggacg cactcttggc tctggggctt tcggacaggt 4141 ggtggaggcc acagctcacg gtctgagcca ttcgcaggcc accatgaaag tggctgtcaa 4201 gatgctgaaa tcgacagcca gaagtagcga gaagcaagcc ttaatgtccg agctgaagat 4261 tatgagtcat cttggacccc acctgaacgt ggtcaacctg ctgggggcct gcaccaaagg 4321 agggcccatc tacatcatca cggaatactg ccgatacggt gatctggtgg actacctgca 4381 ccggaacaaa cacaccttct tgcagcgaca ctccaacaag cattgtccgc ccagtgctga 4441 gctctacagc aacgccctgc cagtggggtt ctccctaccc agccacttga acctgactgg 4501 ggagagtgac ggtggctaca tggatatgag caaggatgaa tctatagatt acgtgcccat 4561 gttggacatg aaaggagaca tcaaatacgc agacattgag tcccccagct acatggcccc 4621 ttatgataac tatgtcccat ctgcccctga aaggacctat cgcgccacct taatcaacga 4681 ctcaccagtg ctcagctaca cagacctcgt gggcttcagc taccaagtgg ccaacggcat 4741 ggacttctta gcctctaaga actgtgttca ccgagacttg gcggccagga atgtgctcat 4801 ctgcgagggc aagctggtca agatctgtga cttcggcctg gctcgagaca tcatgaggga 4861 ctcaaactac atctccaaag gcagcacctt cctgcctctg aagtggatgg ccccagagag 4921 catcttcaac agcctctaca ccactttgag tgatgtctgg tcttttggga tcctactctg 4981 ggagatcttc acactgggtg gcacccctta cccagagctg cccatgaacg accagttcta 5041 caatgccatc aagaggggct accgcatggc ccagcctgct catgcctccg acgagatcta 5101 tgagatcatg cagaaatgct gggaagaaaa gtttgagact cgacccccct tctcccagct 5161 ggtgctgctc ctggagaggc ttctgggtga aggctataaa aagaagtacc agcaggtaga 5221 tgaggagttc ctgaggagtg accatcctgc catcctgagg tcccaagccc gctttccggg 5281 gatccacagc ctccgatccc ctctggacac cagctctgtt ctctacactg ccgtgcagcc 5341 caatgagagt gacaatgact acatcatccc cttacctgac cccaagcctg acgttgctga 5401 tgaaggtctc ccagaggggt cccccagcct tgccagttcc accttgaatg aagtcaacac 5461 ttcctccacc atctcctgcg acagtcccct ggagctccaa gaagagccac agcaagcaga 5521 gcctgaggca caactggagc agccacagga ttcaggctgc ccaggacctc tggctgaagc 5581 agaggacagc ttcctgggat ccatgtaccc atacgacgta cctgattatg caacttatcc 5641 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catagcggcc 5701 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 5761 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 5821 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 5881 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 5941 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 6001 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 6061 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 6121 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 6181 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 6241 ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca tggccatcat 6301 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 6361 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 6421 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 6481 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 6541 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 6601 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 6661 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 6721 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 6781 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 6841 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 6901 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 6961 gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 7021 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 7081 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 7141 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 7201 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 7261 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 7321 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 7381 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 7441 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 7501 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 7561 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 7621 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 7681 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 7741 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 7801 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 7861 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 7921 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 7981 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 8041 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 8101 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 8161 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg 8221 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 8281 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc 8341 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 8401 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 8461 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 8521 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 8581 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 8641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 8701 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 8761 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 8821 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 8881 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 8941 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 9001 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 9061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 9121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 9181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 9241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 9301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 9361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 9421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 9481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 9541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 9601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 9661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 9721 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 9781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 9841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 9901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 9961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 10021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 10081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 10141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 10201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 10261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 10321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 10381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 10441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 10501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 10561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 10621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 10681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 10741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 10801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 10861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 10921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 10981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 11041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 11101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 11161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 11221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 11281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 11341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 11401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 11461 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 11521 acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.