pLV3-CMV-Pdha1-3×FLAG-CopGFP-Puro plasmid

PVT111812

pLV3-CMV-Pdha1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Pdha1(rat)-3×FLAG-CopGFP-Puro
Alias: A0A0G2JW90
Gene lenght: 1128
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pdha1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Pdha1-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Pdha1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGC CCTTGATCcc ggcaagcaga 2341 gtgctggtag cttcccgtaa tttcgcaaat gatgctacat ttgagattaa gaaatgtgac 2401 cttcatcgtc tagaagaggg ccctccagtc accacagtgc tcaccagaga ggatgggctc 2461 aagtactaca ggatgatgca gaccgtgcgc cggatggagc tcaaggcgga tcagctgtat 2521 aagcagaaaa ttattcgtgg tttctgtcac ttgtgtgatg gtcaggaagc ctgctgtgtg 2581 ggcctggagg ctggcataaa ccccacggac catctcatca ctgcctatcg agcccatggc 2641 ttcaccttca ctcggggcct gcctgtccga gcaattcttg cagaattaac aggacgaaga 2701 ggaggctgtg ctaaggggaa aggcggatca atgcacatgt acgccaagaa cttctatggc 2761 ggcaacggta tcgttggagc tcaggtgccc ctgggtgcag gaattgctct ggcctgcaag 2821 tataatggca aagatgaggt ctgtttgaca ttatatggtg atggtgctgc taaccagggt 2881 cagatatttg aagcttacaa tatggcagca ttgtggaaat taccttgtat tttcatctgt 2941 gagaataacc gctatggcat gggcacatct gttgagagag cagcagccag cacggattac 3001 tacaaaagag gagatttcat tcctggactg agggtagatg gaatggatat cttgtgtgtc 3061 cgagaggcaa caaagtttgc agctgcctac tgcaggtctg gtaaggggcc tatcctgatg 3121 gagctacaga cttaccgcta ccatggacat agtatgagtg accctggagt cagttatcgc 3181 actcgagaag aaatccagga agtaagaagt aagagtgacc ctattatgct tctcaaggat 3241 agaatggtga acagcaatct tgcaagtgtt gaagaattaa aggagattga tgtggaagtg 3301 aggaaagaaa tcgaggatgc tgcccagttt gccacggctg atccggagcc gccattggag 3361 gagctaggct atcatatcta cagcagtgac ccacccttcg aagtgcgtgg tgccaaccag 3421 tggatcaagt ttaagtcagt cagtggatcc GACTACAAAG ACCATGACGG TGATTATAAA 3481 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 3541 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 3601 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 3661 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 3721 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 3781 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 3841 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 3901 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 3961 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 4021 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 4081 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 4141 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 4201 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 4261 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 4321 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 4381 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 4441 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 4501 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 4561 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 4621 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 4681 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 4741 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 4801 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 4861 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 4921 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 4981 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5041 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5101 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5161 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5221 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5281 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5341 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5401 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5461 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 5521 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5581 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5641 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5701 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5761 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 5821 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 5881 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 5941 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6001 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 6061 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6121 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6181 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6361 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6421 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6481 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6541 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6601 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 6661 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 6721 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 6781 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6841 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6901 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7621 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7921 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7981 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8041 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8101 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8161 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8221 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8281 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8341 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8401 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8461 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8521 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8581 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8641 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8701 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8761 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8821 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8881 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8941 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9001 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9061 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9121 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9181 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9241 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9301 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9361 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.