pLV3-CMV-PIK3CA-3×Myc-EF1a-CopGFP-Puro plasmid

PVT104071

pLV3-CMV-PIK3CA-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PIK3CA(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_006218.4;CCM4; CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC;p110-alpha; PI3K; PI3K-alpha
Gene lenght: 3204
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PIK3CA-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-PIK3CA-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PIK3CA-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgc ctccacgacc atcatcaggt gaactgtggg gcatccactt 2341 gatgccccca agaatcctag tagaatgttt actaccaaat ggaatgatag tgactttaga 2401 atgcctccgt gaggctacat taataaccat aaagcatgaa ctatttaaag aagcaagaaa 2461 ataccccctc catcaacttc ttcaagatga atcttcttac attttcgtaa gtgttactca 2521 agaagcagaa agggaagaat tttttgatga aacaagacga ctttgtgacc ttcggctttt 2581 tcaacccttt ttaaaagtaa ttgaaccagt aggcaaccgt gaagaaaaga tcctcaatcg 2641 agaaattggt tttgctatcg gcatgccagt gtgtgaattt gatatggtta aagatccaga 2701 agtacaggac ttccgaagaa atattctgaa cgtttgtaaa gaagctgtgg atcttaggga 2761 cctcaattca cctcatagta gagcaatgta tgtctatcct ccaaatgtag aatcttcacc 2821 agaattgcca aagcacatat ataataaatt agataaaggg caaataatag tggtgatctg 2881 ggtaatagtt tctccaaata atgacaagca gaagtatact ctgaaaatca accatgactg 2941 tgtaccagaa caagtaattg ctgaagcaat caggaaaaaa actcgaagta tgttgctatc 3001 ctctgaacaa ctaaaactct gtgttttaga atatcagggc aagtatattt taaaagtgtg 3061 tggatgtgat gaatacttcc tagaaaaata tcctctgagt cagtataagt atataagaag 3121 ctgtataatg cttgggagga tgcccaattt gatgttgatg gctaaagaaa gcctttattc 3181 tcaactgcca atggactgtt ttacaatgcc atcttattcc agacgcattt ccacagctac 3241 accatatatg aatggagaaa catctacaaa atccctttgg gttataaata gtgcactcag 3301 aataaaaatt ctttgtgcaa cctacgtgaa tgtaaatatt cgagacattg ataagatcta 3361 tgttcgaaca ggtatctacc atggaggaga acccttatgt gacaatgtga acactcaaag 3421 agtaccttgt tccaatccca ggtggaatga atggctgaat tatgatatat acattcctga 3481 tcttcctcgt gctgctcgac tttgcctttc catttgctct gttaaaggcc gaaagggtgc 3541 taaagaggaa cactgtccat tggcatgggg aaatataaac ttgtttgatt acacagacac 3601 tctagtatct ggaaaaatgg ctttgaatct ttggccagta cctcatggat tagaagattt 3661 gctgaaccct attggtgtta ctggatcaaa tccaaataaa gaaactccat gcttagagtt 3721 ggagtttgac tggttcagca gtgtggtaaa gttcccagat atgtcagtga ttgaagagca 3781 tgccaattgg tctgtatccc gagaagcagg atttagctat tcccacgcag gactgagtaa 3841 cagactagct agagacaatg aattaaggga aaatgacaaa gaacagctca aagcaatttc 3901 tacacgagat cctctctctg aaatcactga gcaggagaaa gattttctat ggagtcacag 3961 acactattgt gtaactatcc ccgaaattct acccaaattg cttctgtctg ttaaatggaa 4021 ttctagagat gaagtagccc agatgtattg cttggtaaaa gattggcctc caatcaaacc 4081 tgaacaggct atggaacttc tggactgtaa ttacccagat cctatggttc gaggttttgc 4141 tgttcggtgc ttggaaaaat atttaacaga tgacaaactt tctcagtatt taattcagct 4201 agtacaggtc ctaaaatatg aacaatattt ggataacttg cttgtgagat ttttactgaa 4261 gaaagcattg actaatcaaa ggattgggca ctttttcttt tggcatttaa aatctgagat 4321 gcacaataaa acagttagcc agaggtttgg cctgcttttg gagtcctatt gtcgtgcatg 4381 tgggatgtat ttgaagcacc tgaataggca agtcgaggca atggaaaagc tcattaactt 4441 aactgacatt ctcaaacagg agaagaagga tgaaacacaa aaggtacaga tgaagttttt 4501 agttgagcaa atgaggcgac cagatttcat ggatgctcta cagggctttc tgtctcctct 4561 aaaccctgct catcaactag gaaacctcag gcttgaagag tgtcgaatta tgtcctctgc 4621 aaaaaggcca ctgtggttga attgggagaa cccagacatc atgtcagagt tactgtttca 4681 gaacaatgag atcatcttta aaaatgggga tgatttacgg caagatatgc taacacttca 4741 aattattcgt attatggaaa atatctggca aaatcaaggt cttgatcttc gaatgttacc 4801 ttatggttgt ctgtcaatcg gtgactgtgt gggacttatt gaggtggtgc gaaattctca 4861 cactattatg caaattcagt gcaaaggcgg cttgaaaggt gcactgcagt tcaacagcca 4921 cacactacat cagtggctca aagacaagaa caaaggagaa atatatgatg cagccattga 4981 cctgtttaca cgttcatgtg ctggatactg tgtagctacc ttcattttgg gaattggaga 5041 tcgtcacaat agtaacatca tggtgaaaga cgatggacaa ctgtttcata tagattttgg 5101 acactttttg gatcacaaga agaaaaaatt tggttataaa cgagaacgtg tgccatttgt 5161 tttgacacag gatttcttaa tagtgattag taaaggagcc caagaatgca caaagacaag 5221 agaatttgag aggtttcagg agatgtgtta caaggcttat ctagctattc gacagcatgc 5281 caatctcttc ataaatcttt tctcaatgat gcttggctct ggaatgccag aactacaatc 5341 ttttgatgac attgcataca ttcgaaagac cctagcctta gataaaactg agcaagaggc 5401 tttggagtat ttcatgaaac aaatgaatga tgcacatcat ggtggctgga caacaaaaat 5461 ggattggatc ttccacacaa ttaaacagca tgcattgaac gGaGGCGGAG GTAGTGGAGG 5521 AGGGGGAAGC ggaggcggag ggagtggatc cGAACAAAAA CTAATATCAG AGGAAGATTT 5581 GGAACAGAAA CTAATCAGTG AAGAAGATTT AGAGCAAAAG TTAATTTCCG AGGAGGACTT 5641 ATAgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 5701 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 5761 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 5821 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 5881 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 5941 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 6001 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 6061 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 6121 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 6181 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc 6241 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg 6301 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg 6361 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt 6421 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta 6481 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta 6541 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga 6601 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca 6661 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg 6721 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag 6781 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa 6841 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag 6901 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg 6961 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 7021 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 7081 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 7141 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 7201 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 7261 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 7321 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 7381 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 7441 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 7501 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 7561 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 7621 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 7681 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 7741 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 7801 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 7861 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 7921 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 7981 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 8041 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 8101 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 8161 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt 8221 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 8281 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc 8341 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 8401 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 8461 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 8521 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 8581 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 8641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 8701 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat 8761 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 8821 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 8881 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 8941 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 9001 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 9061 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 9121 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 9181 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 9241 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 9301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 9361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 9421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 9481 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 9541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 9601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 9661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 9721 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 9781 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 9841 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 9901 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 9961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 10021 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 10081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 10141 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 10201 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 10261 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 10321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 10381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 10441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 10501 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 10561 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 10621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 10681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 10741 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 10801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 10861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 10921 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 10981 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 11041 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 11101 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 11161 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 11221 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 11281 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 11341 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 11401 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 11461 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 11521 aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???