pLV3-CMV-POLR1A-mCherry-Puro plasmid

PVT106502

pLV3-CMV-POLR1A-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-POLR1A(human)-mCherry-Puro
Alias: NM_015425.6;A190; AFDCIN; HLD27; RPA1; RPA190; RPA194;RPO1-4; RPO14
Gene lenght: 5160
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-POLR1A-mCherry-Puro plasmid Description

pLV3-CMV-POLR1A-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-POLR1A-mCherry-Puro plasmid Sequence

1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 361 gcgctaccgg ACTCAGATCT CGAGCTCAAG CTTCGAATTC TGCAGTCGAC GCCACCatgt 421 tgatctccaa gaacatgccc tggcggcggc tgcagggcat ttccttcggg atgtattcgg 481 ctgaagagct caagaaatta agtgttaaat ccattacgaa ccctcgatac ctggacagcc 541 tggggaaccc atcggcaaac ggcctgtacg atttagcttt gggccctgca gattccaaag 601 aggtgtgctc cacctgcgtg caggacttca gcaactgttc tgggcacctg ggccacattg 661 agctcccact cacagtgtat aaccctctcc tcttcgataa gctgtacctg ctgcttcggg 721 gctcttgttt aaactgccac atgctgactt gtccccgggc cgtgattcac ctcttactct 781 gccagctgag ggttctggaa gtcggggccc tacaagcagt ctacgagctt gagagaattc 841 tgaacaggtt tctggaagaa aatcccgatc cctctgcctc tgaaattcgg gaggaattag 901 aacaatacac aactgaaatt gtgcagaaca acctcctggg gtcccagggc gcacatgtaa 961 agaacgtgtg tgagagcaag agcaagctca ttgctctctt ctggaaggca catatgaatg 1021 ctaagcgctg tccccactgc aagaccgggc gatccgttgt ccgaaaggaa cacaacagca 1081 agttgactat cacgtttcca gccatggtgc acaggacagc tggccagaag gactctgagc 1141 ccctgggaat tgaggaagct cagataggaa aacgaggata cttaacaccc accagtgccc 1201 gcgaacacct ttctgccctg tggaagaatg aaggattctt tctgaactac cttttttcgg 1261 gaatggatga tgatggtatg gaatccagat tcaatcccag tgtgttcttt ctagatttct 1321 tggtggtgcc gccctcaagg tatcgcccag tcagtcgcct aggagaccag atgtttacta 1381 atggccagac ggtgaacttg caggctgtca tgaaggatgt agttctgatt cgaaaacttc 1441 tggcattgat ggcccaagaa cagaagttgc cagaggaagt ggccacaccc actacagatg 1501 aggaaaaaga ctctttgatt gctattgacc gatccttttt gagtacactt ccaggccagt 1561 ccctcataga caaactttac aacatttgga ttcgccttca gagccacgtc aatattgtgt 1621 ttgatagcga gatggacaaa ctaatgatgg acaagtaccc aggcattagg cagatcctgg 1681 agaagaaaga aggcctgttc cgaaaacaca tgatgggaaa gcgagtggac tacgctgcgc 1741 gctcagtcat ctgcccagac atgtacatca acaccaacga aattggaatt cccatggtgt 1801 ttgccacaaa actgacctac ccacagccag ttaccccatg gaatgttcag gaacttaggc 1861 aagcggtcat caacggccct aatgtgcacc caggagcctc catggtcatc aatgaggacg 1921 gcagccgcac agccctgagc gctgtggaca tgacccagcg agaggccgtg gccaagcagc 1981 ttctgacccc agccacgggg gcacctaagc cccaggggac aaaaattgtg tgccggcatg 2041 tgaagaatgg ggacattctg ctactgaacc gacagcccac actgcacaga ccctccatcc 2101 aggcccaccg tgcccgcatc ctgcctgaag agaaagtgct gcggctccac tatgccaact 2161 gcaaggccta taatgccgac tttgatggag acgagatgaa tgcccatttc ccccagagtg 2221 agctgggccg ggccgaggcc tacgtcctgg cctgcactga tcagcagtac cttgttccca 2281 aggatggcca accattggcg ggactgatcc aggatcacat ggtttcaggg gcaagcatga 2341 ctactcgggg ttgctttttc acccgggagc actatatgga gctggtgtac cgaggactca 2401 cggacaaagt ggggcgcgtg aagctccttt ctccttccat cctgaagccc tttccgctgt 2461 ggacaggaaa acaggttgtg tcaacgctgc tcataaatat aatcccagag gaccacatcc 2521 cactgaactt atctggaaag gcgaaaatca ctgggaaagc ctgggtgaag gaaactcctc 2581 gatccgttcc tggctttaac cctgactcga tgtgcgagtc ccaggtgatc atcagggaag 2641 gggagctgct ctgcggagtg ctggacaagg cgcactatgg gagctccgcc tacggcctgg 2701 tccactgctg ctatgagatc tatggaggcg agaccagcgg caaggttcta acctgcctgg 2761 cccgcctctt caccgcctac ctgcagctct acagaggctt caccttgggc gtggaagaca 2821 ttttggtgaa gccaaaggca gatgtcaaga ggcaacgtat cattgaagaa tccacccact 2881 gcgggcccca ggctgtcagg gctgcattaa acctgccaga agccgcatca tatgatgagg 2941 tccgaggaaa atggcaggat gcccatctgg gcaaggacca gagggatttt aacatgattg 3001 atctgaagtt caaggaggaa gtgaaccatt acagcaatga gattaacaag gcatgcatgc 3061 cttttggcct acacagacag ttcccagaga acagcctgca gatgatggtg cagtcgggag 3121 ccaaaggttc aactgtgaac acgatgcaga tctcgtgcct gctgggccag attgaactgg 3181 aaggtcggag acccccgctg atggcgtctg gcaagtcact gccctgcttt gagccttatg 3241 agttcacccc cagggctggt ggctttgtca ctggcaggtt cctcaccggc atcaaacctc 3301 ctgagttctt cttccactgc atggcaggac gagagggcct ggtggacact gctgtgaaaa 3361 ccagccgctc aggctatctc caaaggtgca tcatcaagca cctagagggg ctggtcgtgc 3421 agtatgatct cacggtccgt gacagtgacg gcagtgtggt gcagttcctg tatggggagg 3481 atggcctgga catccccaag acacagttcc tgcagcccaa gcagttcccc ttcctggcca 3541 gcaactacga ggtgataatg aaatcacagc atctccatga agttttatcc agagcagatc 3601 ccaaaaaagc tctccaccac ttcagagcta tcaaaaaatg gcaaagcaag caccccaaca 3661 ccctgctgag aagaggcgcc ttcttgagtt attcccagaa aattcaggaa gctgtgaaag 3721 ccctgaaact tgagagtgaa aaccgcaatg gccgcagccc tgggactcag gagatgctga 3781 ggatgtggta tgagttggat gaggaaagcc gaaggaaata ccagaagaag gcggccgctt 3841 gtcctgaccc cagtctgtct gtctggcgtc ctgacatcta ctttgcatca gtgtcagaaa 3901 catttgaaac aaaggttgat gactacagtc aagagtgggc agctcaaaca gagaagagtt 3961 atgagaaatc agagctttct ctcgacaggt tgaggacctt gctgcagctg aagtggcagc 4021 gctcactgtg tgagccgggc gaggctgtgg gcctgctggc tgcccagagc atcggagagc 4081 cctccaccca gatgaccctc aacaccttcc actttgcagg cagaggcgag atgaacgtca 4141 ccctgggcat tccaaggttg cgggagattc tcatggtggc cagcgccaac atcaagacac 4201 ccatgatgag cgtgcccgtg ctcaacacca agaaagccct gaagagagtg aaaagcctga 4261 agaagcaact caccagggtg tgcttggggg aggtgttgca gaaaattgac gtccaggagt 4321 ccttctgtat ggaagaaaaa cagaacaaat tccaggtgta ccagctgcgg tttcagttcc 4381 tgccacatgc atattaccag caggagaagt gcctgagacc cgaggacatc ctgcgcttca 4441 tggaaacaag attctttaaa cttctgatgg aatccatcaa aaagaagaat aataaagcat 4501 cagctttcag gaacgtaaac actcgaagag ctacacagcg ggatctggac aacgctgggg 4561 agttggggag gagtcgggga gagcaggagg gtgatgagga agaggagggg cacattgtgg 4621 atgctgaagc tgaggagggg gacgccgatg cctctgatgc caaacgcaag gagaagcagg 4681 aggaggaggt tgattatgag agtgaggaag aggaggagag ggagggcgag gagaacgacg 4741 atgaagacat gcaggaggaa cgaaatcccc acagggaagg tgctcgaaag acccaagagc 4801 aagatgaaga ggtgggctta ggcactgagg aggacccgtc ccttcccgcc ctcctgacgc 4861 agccccggaa acccacccac agccaggagc cccaggggcc cgaggccatg gagcgccggg 4921 tccaggctgt gcgtgagatc cacccgttca tagatgacta ccagtacgac accgaggaga 4981 gcctgtggtg ccaggtgaca gtgaagctcc ctctgatgaa gatcaacttt gacatgagct 5041 ccctggtagt atctttggcc catggtgccg tcatctatgc gaccaagggc atcactcggt 5101 gcctcctgaa tgaaacaacc aacaataaga acgagaagga gcttgtgcta aacacagaag 5161 gaatcaacct cccagagcta ttcaagtatg cagaggtcct ggatctgcgc cgcctctact 5221 ccaacgacat ccacgccata gccaacacgt atggcattga ggccgcgctg cgggtgatcg 5281 agaaggagat caaggatgtg tttgccgtgt atggcatcgc ggtcgaccct cgccatctct 5341 ccctggttgc tgattatatg tgcttcgagg gtgtttacaa gccactgaat cgctttggga 5401 tccggtcaaa ctcttccccg ctacagcaga tgacatttga aaccagcttc cagtttctga 5461 agcaagccac catgctggga tcccacgatg agctgaggtc tccttctgcc tgccttgtgg 5521 tcgggaaggt cgtcaggggc gggacaggcc tgttcgagct caagcagcct ctgagaggtg 5581 gaggcggatc aggaggcggt ggatcACCGG TCGCCACCat ggtgagcaag ggcgaggagg 5641 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 5701 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 5761 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 5821 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 5881 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 5941 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 6001 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 6061 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 6121 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 6181 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 6241 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 6301 ccggcggcat ggacgagctg tacaagtGAA ttctcgacct cgagacaaat ggcagtattc 6361 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 6421 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 6481 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt 6541 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg 6601 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg 6661 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc 6721 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc 6781 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg 6841 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg 6901 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc 6961 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga 7021 ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga ccgagtacaa 7081 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 7141 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 7201 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 7261 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 7321 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 7381 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 7441 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 7501 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 7561 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 7621 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga 7681 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcggtacctt taagaccaat 7741 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 7801 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt 7861 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 7921 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7981 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 8041 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 8101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 8161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 8221 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc 8281 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 8341 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg 8401 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac 8461 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 8521 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 8581 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 8641 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 8701 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 8761 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 8821 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 8881 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 8941 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 9001 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 9061 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 9121 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 9181 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 9241 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 9301 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 9361 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 9421 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 9481 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 9541 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 9601 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 9661 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 9721 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 9781 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 9841 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 9901 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 9961 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 10021 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 10081 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 10141 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 10201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 10261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 10321 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 10381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 10441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 10501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 10561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 10621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 10681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 10741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 10801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 10861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 10921 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 10981 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 11041 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 11101 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 11161 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 11221 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag 11281 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta 11341 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc 11401 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat 11461 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac 11521 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 11581 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 11641 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg 11701 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag 11761 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 11821 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 11881 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata 11941 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat 12001 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag 12061 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga 12121 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca 12181 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg 12241 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc 12301 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc 12361 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta 12421 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt 12481 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga 12541 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct 12601 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg 12661 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca 12721 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa 12781 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg 12841 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt 12901 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc 12961 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag 13021 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac 13081 tagcctcgag cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg 13141 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag 13201 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 13261 aaattatgtt ttaaaatgga ctatca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???