pLV3-CMV-Rarg-EGFP-Bleo plasmid

PVT100898

pLV3-CMV-Rarg-EGFP-Bleo plasmid Information

Plasmid name: pLV3-CMV-Rarg(mouse)-EGFP-Bleo
Alias: NM_011244.5;Nr1b3; RAR-gamma; RARD; RARgamma2
Gene lenght: 1374bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Bleo
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pLV3-CMV-Rarg-EGFP-Bleo plasmid Description

pLV3-CMV-Rarg-EGFP-Bleo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Rarg-EGFP-Bleo plasmid Sequence

1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag 2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg 2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt 2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc 2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccGAAT 2821 TGGCTAGGCG GCGCGCCTTA ATTAAGGATC CATGTATCCG TACGACGTTC CAGACTACGC 2881 ATACCCGTAC GATGTGCCGG ACTATGCGTA CCCGTATGAC GTCCCGGACT ATGCTatggc 2941 caccaataag gagagactct ttgcgcccgg tgccctgggg cctggatctg gttacccagg 3001 agcaggcttc ccattcgcct tcccaggtgc actcagaggg tcgccaccat ttgagatgct 3061 gagccctagc ttccggggcc tgggccagcc tgacctcccc aaggagatgg cttctctctc 3121 ggtggagaca cagagcacca gctcggagga gatggtaccc agctctccct cacccccacc 3181 acctcctcgg gtctataagc catgctttgt atgcaatgac aagtcttctg gctaccacta 3241 tggggtcagc tcctgtgaag gctgcaaggg cttcttcaga cgcagcattc agaaaaacat 3301 ggtgtataca tgtcaccgtg acaaaaactg tatcatcaac aaggtcacca gaaatcgatg 3361 ccagtactgc aggctacaaa agtgtttcga agtgggcatg tccaaggaag ctgtaaggaa 3421 cgatcgaaac aagaagaaaa aggaggtaaa agaggagggc tcgcccgaca gctatgaact 3481 gagtccacag ttagaggaac tcatcaccaa ggtcagcaaa gcccaccagg agacttttcc 3541 ctcactctgc cagctgggca agtacaccac gaactccagt gcagatcacc gggtgcagct 3601 ggacctgggg ctgtgggaca agttcagcga gctggccacc aaatgcatca tcaagattgt 3661 ggagtttgcg aagcggctgc ctggttttac agggctcagc attgccgacc agatcacgct 3721 gctcaaggct gcttgtctgg acatcctaat gctgcggatc tgtacaaggt ataccccaga 3781 gcaggacact atgacattct cggatgggct gaccctgaac cgaacccaga tgcacaatgc 3841 tggctttggg ccccttacag acctcgtctt tgcctttgcc gggcagctgc tgcccctgga 3901 gatggatgac accgagactg ggctacttag tgctatctgc ctcatctgtg gagaccgaat 3961 ggacctggaa gagcccgaga aggtggacaa gctgcaggag cccctgctgg aagccctgag 4021 gctctatgcc cggcgacgga gacccagcca accctacatg ttcccaagga tgctgatgaa 4081 aatcaccgac ctccggggca tcagcactaa gggagcagaa agggctataa ccctgaagat 4141 ggagattcca ggcccgatgc cacccctgat ccgagagatg ctggagaacc cggagatgtt 4201 tgaggacgac tcctcgaagc ctggccccca ccccaaggct tccagtgagg acgaagctcc 4261 agggggccag ggcaaaaggg gccaaagtcc ccaacctgac caggggcccg agggcagagg 4321 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccagtgagca agggcgagga 4381 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 4441 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 4501 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 4561 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 4621 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 4681 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 4741 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 4801 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 4861 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 4921 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 4981 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 5041 cgccgggatc actctcggca tggacgagct gtacaaggaa ttctaagaat tcgatatcaa 5101 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 5161 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 5221 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 5281 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 5341 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 5401 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 5461 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 5521 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 5581 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 5641 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca 5701 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc 5761 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc 5821 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 5881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat 5941 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc 6001 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga 6061 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag 6121 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct 6181 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga 6241 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6301 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6361 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc 6421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 6481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 6541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 6601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 6661 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 6721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 6781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 6841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 6901 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 6961 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 7021 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 7081 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 7141 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 7201 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 7261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 7321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 7381 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 7441 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 7501 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata 7561 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg 7621 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc 7681 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc 7741 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc 7801 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc 7861 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac 7921 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat 7981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 8041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 8101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 8161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 8221 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 8281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 8341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 8401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 8461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 8521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 8581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 8641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 8701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 8761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 8821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 8881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 8941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 9001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 9061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 9121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 9181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 9241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 9301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 9361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 9421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 9481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 9541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 9601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 9661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 9721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 9781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 9841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 9901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 9961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 10021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 10081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 10141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 10201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 10261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 10321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 10381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc 10441 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 10501 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta 10561 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg 10621 cgctgcttcg cgatgtacgg gccagatata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???