pLV3-CMV-SDHC-3×FLAG-CopGFP-Puro plasmid

PVT104988

pLV3-CMV-SDHC-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SDHC(human)-3×FLAG-CopGFP-Puro
Alias: NM_003001.5;CYB560; CYBL; PGL3; PPGL3; QPS1; SDH3;
Gene lenght: 507
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SDHC-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SDHC-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SDHC-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ctgcgctgtt gctgagacac 2341 gttggtcgtc attgcctccg agcccacttt agccctcagc tctgtatcag aaatgctgtt 2401 cctttgggaa ccacggccaa agaagagatg gagcggttct ggaataagaa tataggttca 2461 aaccgtcctc tgtctcccca cattactatc tacagttggt ctcttcccat ggcgatgtcc 2521 atctgccacc gtggcactgg tattgctttg agtgcagggg tctctctttt tggcatgtcg 2581 gccctgttac tccctgggaa ctttgagtct tatttggaac ttgtgaagtc cctgtgtctg 2641 gggccagcac tgatccacac agctaagttt gcacttgtct tccctctcat gtatcatacc 2701 tggaatggga tccgacactt gatgtgggac ctaggaaaag gcctgaagat tccccagcta 2761 taccagtctg gagtggttgt cctggttctt actgtgttgt cctctatggg gctggcagcc 2821 atgggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 2881 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3121 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3181 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 3241 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 3301 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 3361 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 3421 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 3481 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 3541 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 3601 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 3661 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 3721 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 3781 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 3841 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 3901 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 3961 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4021 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4081 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4141 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4201 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 4261 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4321 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4381 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4441 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4501 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4561 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4621 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4681 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 4741 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4801 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4861 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 4921 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 4981 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5041 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5101 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5161 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 5221 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 5281 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 5341 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 5401 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 5461 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5521 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 5581 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 5641 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5701 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5761 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 5821 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 5881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5941 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6001 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6061 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6121 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 6181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6241 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 7621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 7801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 7861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 7981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 8341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 8401 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 8461 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 8521 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 8581 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 8641 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 8701 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 8761 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???