pLV3-CMV-SHCBP1-3×FLAG-CopGFP-Puro plasmid

PVT98648

pLV3-CMV-SHCBP1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SHCBP1(human)-3×FLAG-CopGFP-Puro
Alias: NM_024745.5;PAL
Gene lenght: 2016bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SHCBP1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SHCBP1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SHCBP1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ctgacgggtc gctgacgggc 2341 ggcggtctgg aggcagcggc catggcgccg gagcgcatgg gctgggcggt ggagcaggag 2401 ctggcgtctc tggagaaagg tttgttccaa gatgaagatt catgcagtga ttgtagctac 2461 cgtgataaac caggttctag tttacaaagt tttatgccag aaggaaaaac ctttttccca 2521 gaaattttcc aaacaaatca acttttgttc tatgagcgat tcagagccta tcaagattac 2581 attttagctg actgcaaggc ctctgaggta caggaattca cagctgagtt cttggagaag 2641 gtccttgagc catctggatg gcgggcagtc tggcacacta atgtgttcaa ggtgctggtt 2701 gagatcacag atgtggactt tgcagccttg aaggcagtgg tgaggcttgc tgaaccatac 2761 ctctgtgact ctcaagtgag cacttttacc atggagtgca tgaaggagct ccttgatctg 2821 aaggagcatc ggttgcccct gcaggagctg tgggtggtgt ttgatgattc aggagtgttt 2881 gaccagacag cccttgcaat tgagcatgtc agatttttct accaaaacat ttggaggagt 2941 tgggatgaag aagaggagga tgaatacgat tattttgtca gatgtgttga acctcgatta 3001 agattgcatt atgacattct tgaagaccga gttccatcag gacttattgt tgactaccac 3061 aatctgttgt ctcaatgtga ggagagttac aggaaatttt taaatctgag aagcagtttg 3121 tcaaattgta actctgattc cgagcaggaa aatatctcca tggtggaagg gttaaaattg 3181 tattcggaga tggaacagtt gaaacaaaag ctgaaactca ttgagaatcc tttgttgagg 3241 tatgtgtttg gttatcagaa gaattctaac atccaagcaa agggtgtccg ttccagcggt 3301 cagaagatca ctcatgtggt ctcctccacc atgatggctg gtctcctgcg gtccctgctt 3361 acggacaggc tttgccagga gcctggtgag gaagaaagag aaattcagtt ccatagtgat 3421 ccattgtctg ctataaatgc ctgcttcgaa ggtgacactg ttattgtttg tcctggccat 3481 tatgtggtac atggcacttt ctccattgct gactccattg agttggaagg atatggccta 3541 ccagatgaca ttgtgataga aaagaggggc aaaggcgaca cttttgtgga ctgcactggt 3601 gctgatatta aaatctcagg cataaaattt gttcagcatg atgctgtaga gggaatctta 3661 attgttcacc gtggtaagac tacgctggaa aactgtgtgc tgcagtgtga gacgaccgga 3721 gtcacagtgc ggacatcagc agagtttcta atgaagaact cggatttata tggcgccaag 3781 ggtgctggta tagaaatcta ccctgggagt cagtgcaccc tgagtgacaa tgggatccat 3841 cactgcaagg aagggatcct cattaaggac ttcttagatg aacattatga cattcccaag 3901 atatccatgg tgaataatat aatacataat aatgaaggtt atggtgttgt cttggtgaaa 3961 cctacaatct tctctgacct gcaagaaaat gctgaagatg gaactgaaga aaataaagcg 4021 cttaaaattc agacaagtgg agagccagat gtggctgaaa gagtggatct agaagagctg 4081 attgagtgtg caactggtaa aatggagctt tgtgcaagaa ctgacccttc tgagcaagtc 4141 gagggaaatt gtgaaattgt aaatgaacta attgctgcct ccacacagaa aggccagata 4201 aagaagaaaa ggttgagtga actggggatc acgcaagctg atgacaactt aatgtcacag 4261 gagatgtttg ttgggattgt ggggaaccag ttcaagtgga atgggaaagg tagttttggc 4321 acatttcttt tcggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 4381 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 4441 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 4501 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 4561 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 4621 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 4681 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 4741 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4801 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 4861 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 4921 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 4981 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 5041 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 5101 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 5161 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 5221 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 5281 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 5341 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 5401 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 5461 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 5521 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 5581 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 5641 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 5701 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 5761 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 5821 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 5881 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5941 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 6001 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 6061 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 6121 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 6181 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 6241 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 6301 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 6361 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 6421 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6481 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6541 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 6601 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 6661 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6721 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 6781 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6841 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6901 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 6961 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 7021 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 7081 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 7141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 7201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 7261 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 7321 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 7381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 7441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 7501 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 7561 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 7621 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 7681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 9481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 9541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 9841 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9901 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9961 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 10021 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 10081 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 10141 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 10201 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 10261 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???