pLV3-CMV-SLC2A1-3×FLAG-Hyg plasmid

PVT106091

pLV3-CMV-SLC2A1-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-SLC2A1(human)-3×FLAG-Hyg
Alias: NM_006516.4;CSE; DYT17; DYT18; DYT9; EIG12; GLUT;GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN
Gene lenght: 1476
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLC2A1-3×FLAG-Hyg plasmid Description

pLV3-CMV-SLC2A1-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-SLC2A1-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatggagcc cagcagcaag aagctgacgg gtcgcctcat 2341 gctggccgtg ggaggagcag tgcttggctc cctgcagttt ggctacaaca ctggagtcat 2401 caatgccccc cagaaggtga tcgaggagtt ctacaaccag acatgggtcc accgctatgg 2461 ggagagcatc ctgcccacca cgctcaccac gctctggtcc ctctcagtgg ccatcttttc 2521 tgttgggggc atgattggct ccttctctgt gggccttttc gttaaccgct ttggccggcg 2581 gaattcaatg ctgatgatga acctgctggc cttcgtgtcc gccgtgctca tgggcttctc 2641 gaaactgggc aagtcctttg agatgctgat cctgggccgc ttcatcatcg gtgtgtactg 2701 cggcctgacc acaggcttcg tgcccatgta tgtgggtgaa gtgtcaccca cagcccttcg 2761 tggggccctg ggcaccctgc accagctggg catcgtcgtc ggcatcctca tcgcccaggt 2821 gttcggcctg gactccatca tgggcaacaa ggacctgtgg cccctgctgc tgagcatcat 2881 cttcatcccg gccctgctgc agtgcatcgt gctgcccttc tgccccgaga gtccccgctt 2941 cctgctcatc aaccgcaacg aggagaaccg ggccaagagt gtgctaaaga agctgcgcgg 3001 gacagctgac gtgacccatg acctgcagga gatgaaggaa gagagtcggc agatgatgcg 3061 ggagaagaag gtcaccatcc tggagctgtt ccgctccccc gcctaccgcc agcccatcct 3121 catcgctgtg gtgctgcagc tgtcccagca gctgtctggc atcaacgctg tcttctatta 3181 ctccacgagc atcttcgaga aggcgggggt gcagcagcct gtgtatgcca ccattggctc 3241 cggtatcgtc aacacggcct tcactgtcgt gtcgctgttt gtggtggagc gagcaggccg 3301 gcggaccctg cacctcatag gcctcgctgg catggcgggt tgtgccatac tcatgaccat 3361 cgcgctagca ctgctggagc agctaccctg gatgtcctat ctgagcatcg tggccatctt 3421 tggctttgtg gccttctttg aagtgggtcc tggccccatc ccatggttca tcgtggctga 3481 actcttcagc cagggtccac gtccagctgc cattgccgtt gcaggcttct ccaactggac 3541 ctcaaatttc attgtgggca tgtgcttcca gtatgtggag caactgtgtg gtccctacgt 3601 cttcatcatc ttcactgtgc tcctggttct gttcttcatc ttcacctact tcaaagttcc 3661 tgagactaaa ggccggacct tcgatgagat cgcttccggc ttccggcagg ggggagccag 3721 ccaaagtgac aagacacccg aggagctgtt ccatcccctg ggggctgatt cccaagtgCT 3781 CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 3841 CGATGACAAG TAGcggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 3901 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 3961 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 4021 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 4081 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 4141 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 4201 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 4261 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 4321 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 4381 agatccaagc tgtgaccggc gcctacgtcg acatgaaaaa gcctgaactc accgcgacgt 4441 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg 4501 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg 4561 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg 4621 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt 4681 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg 4741 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga 4801 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt 4861 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg 4921 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg 4981 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc 5041 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg 5101 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg 5161 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg 5221 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc 5281 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg 5341 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa 5401 accgacgccc cagcactcgt ccgagggcaa aggaataggt cgacaatcaa cctctggatt 5461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 5521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 5581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 5641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 5701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 5761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 5821 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 5881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 5941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 6001 cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac 6061 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 6121 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 6181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 6241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 6301 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 6361 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 6421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 6481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 6541 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 6601 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 6661 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat 6721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 6841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 6961 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7021 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7081 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7141 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7321 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7501 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7561 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7621 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7681 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7741 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7801 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7861 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7921 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7981 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8041 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8101 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8161 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8221 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8281 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8341 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8401 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8461 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8521 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8581 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8641 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8701 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8761 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8881 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 8941 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 9001 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 9061 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 9121 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 9181 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 9241 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 9301 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???