pLV3-CMV-SLC3A2-3×FLAG-Hyg plasmid

PVT91645

pLV3-CMV-SLC3A2-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-SLC3A2(human)-3×FLAG-Hyg
Alias: NM_002394.6;4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1;NACAE
Gene lenght: 1890bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLC3A2-3×FLAG-Hyg plasmid Description

pLV3-CMV-SLC3A2-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SLC3A2-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatggagct acagcctcct gaagcctcga tcgccgtcgt 2341 gtcgattccg cgccagttgc ctggctcaca ttcggaggct ggtgtccagg gtctcagcgc 2401 gggggacgac tcagagttgg ggtctcactg tgttgcccag actggtctcg aactcttggc 2461 ctcaggtgat cctcttccct cagcttccca gaatgccgag atgatagaga cggggtctga 2521 ctgtgttacc caggctggtc ttcaactctt ggcctcaagt gatcctcctg ccttagcttc 2581 caagaatgct gaggttacag gcaccatgag ccaggacacc gaggtggata tgaaggaggt 2641 ggagctgaat gagttagagc ccgagaagca gccgatgaac gcggcgtctg gggcggccat 2701 gtccctggcg ggagccgaga agaatggtct ggtgaagatc aaggtggcgg aagacgaggc 2761 ggaggcggca gccgcggcta agttcacggg cctgtccaag gaggagctgc tgaaggtggc 2821 aggcagcccc ggctgggtac gcacccgctg ggcactgctg ctgctcttct ggctcggctg 2881 gctcggcatg cttgctggtg ccgtggtcat aatcgtgcga gcgccgcgtt gtcgcgagct 2941 accggcgcag aagtggtggc acacgggcgc cctctaccgc atcggcgacc ttcaggcctt 3001 ccagggccac ggcgcgggca acctggcggg tctgaagggg cgtctcgatt acctgagctc 3061 tctgaaggtg aagggccttg tgctgggtcc aattcacaag aaccagaagg atgatgtcgc 3121 tcagactgac ttgctgcaga tcgaccccaa ttttggctcc aaggaagatt ttgacagtct 3181 cttgcaatcg gctaaaaaaa agagcatccg tgtcattctg gaccttactc ccaactaccg 3241 gggtgagaac tcgtggttct ccactcaggt tgacactgtg gccaccaagg tgaaggatgc 3301 tctggagttt tggctgcaag ctggcgtgga tgggttccag gttcgggaca tagagaatct 3361 gaaggatgca tcctcattct tggctgagtg gcaaaatatc accaagggct tcagtgaaga 3421 caggctcttg attgcgggga ctaactcctc cgaccttcag cagatcctga gcctactcga 3481 atccaacaaa gacttgctgt tgactagctc atacctgtct gattctggtt ctactgggga 3541 gcatacaaaa tccctagtca cacagtattt gaatgccact ggcaatcgct ggtgcagctg 3601 gagtttgtct caggcaaggc tcctgacttc cttcttgccg gctcaacttc tccgactcta 3661 ccagctgatg ctcttcaccc tgccagggac ccctgttttc agctacgggg atgagattgg 3721 cctggatgca gctgcccttc ctggacagcc tatggaggct ccagtcatgc tgtgggatga 3781 gtccagcttc cctgacatcc caggggctgt aagtgccaac atgactgtga agggccagag 3841 tgaagaccct ggctccctcc tttccttgtt ccggcggctg agtgaccagc ggagtaagga 3901 gcgctcccta ctgcatgggg acttccacgc gttctccgct gggcctggac tcttctccta 3961 tatccgccac tgggaccaga atgagcgttt tctggtagtg cttaactttg gggatgtggg 4021 cctctcggct ggactgcagg cctccgacct gcctgccagc gccagcctgc cagccaaggc 4081 tgacctcctg ctcagcaccc agccaggccg tgaggagggc tcccctcttg agctggaacg 4141 cctgaaactg gagcctcacg aagggctgct gctccgcttc ccctacgcgg ccggtggagg 4201 cggatcaCTC GAGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 4261 CAAGGATGAC GATGACAAGT AGcggccgca aggatctgcg atcgctccgg tgcccgtcag 4321 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4381 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4441 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4501 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4561 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 4621 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4681 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4741 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4801 cgccgttaca gatccaagct gtgaccggcg cctacgtcga catgaaaaag cctgaactca 4861 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 4921 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 4981 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact 5041 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc 5101 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg 5161 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc 5221 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat 5281 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg 5341 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg 5401 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg 5461 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat 5521 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc 5581 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc 5641 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag 5701 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta 5761 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg 5821 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataggtc gacaatcaac 5881 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 5941 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 6001 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 6061 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 6121 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 6181 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 6241 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 6301 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 6361 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 6421 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca 6481 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 6541 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg 6601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 6721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 6781 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 6841 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 6901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6961 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 7021 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 7081 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 7141 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 7201 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 7261 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7981 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 8101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 8161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 8221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 8281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 8341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 8401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 9121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 9181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 9241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9301 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 9361 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 9421 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 9481 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 9541 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 9601 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 9661 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 9721 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???