pLV3-CMV-SRC-3×HA-CopGFP-Puro plasmid

PVT96307

pLV3-CMV-SRC-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SRC(human)-3×HA-CopGFP-Puro
Alias: NM_198291.3;ASV; c-SRC; p60-Src; SRC1; THC6
Gene lenght: 1608bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SRC-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-SRC-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SRC-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gtagcaacaa gagcaagccc 2341 aaggatgcca gccagcggcg ccgcagcctg gagcccgccg agaacgtgca cggcgctggc 2401 gggggcgctt tccccgcctc gcagaccccc agcaagccag cctcggccga cggccaccgc 2461 ggccccagcg cggccttcgc ccccgcggcc gccgagccca agctgttcgg aggcttcaac 2521 tcctcggaca ccgtcacctc cccgcagagg gcgggcccgc tggccggtgg agtgaccacc 2581 tttgtggccc tctatgacta tgagtctagg acggagacag acctgtcctt caagaaaggc 2641 gagcggctcc agattgtcaa caacacagag ggagactggt ggctggccca ctcgctcagc 2701 acaggacaga caggctacat ccccagcaac tacgtggcgc cctccgactc catccaggct 2761 gaggagtggt attttggcaa gatcaccaga cgggagtcag agcggttact gctcaatgca 2821 gagaacccga gagggacctt cctcgtgcga gaaagtgaga ccacgaaagg tgcctactgc 2881 ctctcagtgt ctgacttcga caacgccaag ggcctcaacg tgaagcacta caagatccgc 2941 aagctggaca gcggcggctt ctacatcacc tcccgcaccc agttcaacag cctgcagcag 3001 ctggtggcct actactccaa acacgccgat ggcctgtgcc accgcctcac caccgtgtgc 3061 cccacgtcca agccgcagac tcagggcctg gccaaggatg cctgggagat ccctcgggag 3121 tcgctgcggc tggaggtcaa gctgggccag ggctgctttg gcgaggtgtg gatggggacc 3181 tggaacggta ccaccagggt ggccatcaaa accctgaagc ctggcacgat gtctccagag 3241 gccttcctgc aggaggccca ggtcatgaag aagctgaggc atgagaagct ggtgcagttg 3301 tatgctgtgg tttcagagga gcccatttac atcgtcacgg agtacatgag caaggggagt 3361 ttgctggact ttctcaaggg ggagacaggc aagtacctgc ggctgcctca gctggtggac 3421 atggctgctc agatcgcctc aggcatggcg tacgtggagc ggatgaacta cgtccaccgg 3481 gaccttcgtg cagccaacat cctggtggga gagaacctgg tgtgcaaagt ggcggacttt 3541 gggctggctc ggctcattga agacaatgag tacacggcgc ggcaaggtgc caaattcccc 3601 atcaagtgga cggctccaga agctgccctc tatggccgct tcaccatcaa gtcggacgtg 3661 tggtccttcg ggatcctgct gactgagctc accacaaagg gacgggtgcc ctaccctggg 3721 atggtgaacc gcgaggtgct ggaccaggtg gagcggggct accggatgcc ctgcccgccg 3781 gagtgtcccg agtccctgca cgacctcatg tgccagtgct ggcggaagga gcctgaggag 3841 cggcccacct tcgagtacct gcaggccttc ctggaggact acttcacgtc caccgagccc 3901 cagtaccagc ccggggagaa cctcCTCGAG TACCCATACG ACGTACCTGA TTATGCAACT 3961 TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA CTACGCATAG 4021 ggatccCTGC GATATCCAGC gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 4081 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4141 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4201 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4261 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4321 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 4381 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4441 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4501 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4561 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4621 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4681 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4741 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4801 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4861 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4921 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4981 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 5041 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 5101 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 5161 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 5221 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 5281 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 5341 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 5401 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5461 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5521 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5581 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5641 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5701 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5761 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5821 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5881 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5941 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6001 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6061 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6121 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6181 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6241 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6301 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6361 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 6421 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6481 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6541 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 6601 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6661 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 6721 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 6781 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 6841 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 6901 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 6961 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7081 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 7141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 7201 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 7261 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 7321 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7381 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7441 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8101 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 8161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9421 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 9481 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 9541 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 9601 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 9661 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 9721 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 9781 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 9841 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 9901 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???