pLV3-CMV-SRRM1-3×FLAG-CopGFP-Puro plasmid

PVT101305

pLV3-CMV-SRRM1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SRRM1(human)-3×FLAG-CopGFP-Puro
Alias: NM_001303448.2;160-KD; POP101; SRM160
Gene lenght: 2748bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: Low
Induction:

pLV3-CMV-SRRM1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SRRM1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SRRM1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg acgcgggatt tttccgcgga 2341 acaagtgcag aacaggataa tcggttcagc aacaaacaga agaaactact gaagcagctg 2401 aaatttgcag aatgcctaga aaaaaaggtg gacatgagca aagtaaattt ggaggttata 2461 aagccttgga taacaaaaag agtaacggaa atccttgggt ttgaagatga tgttgtgatt 2521 gagtttatat tcaaccagct ggaagtgaag aatccagact ccaaaatgat gcaaatcaac 2581 ctgactggat ttttgaatgg aaaaaatgct cgagaattta tgggagaact gtggcccctg 2641 ctgctaagtg cacaagaaaa catcgcggga atcccttctg ctttcctaga actgaagaaa 2701 gaagaaataa aacaaagaca gattgaacaa gaaaaactgg catctatgaa aaagcaagat 2761 gaagacaaag ataaaagaga taaggaagaa aaagaaagca gcagagaaaa aagggagcgg 2821 tctcgtagcc caagaagacg caaatccaga tctccttccc ctagaagacg atcttcccct 2881 gtcaggagag agagaaagcg cagtcattct cgatctcccc gtcacagaac caagagccgg 2941 agtccttccc ctgctccaga aaagaaggaa aaaactccag agctcccaga accttcagtg 3001 aaagtaaaag aaccttcagt acaagaggct acttctacta gtgacattct gaaagttccc 3061 aaacctgaac ctataccaga gcctaaagaa ccttctccgg aaaaaaattc caaaaaagaa 3121 aaggagaagg agaagacccg accacgatct cggtcacgct ccaaatcaag atcccggacg 3181 cggtcccgct ctccttctca cactcgacct agacggcgcc atagatcccg atcaagatcg 3241 tattcaccta gaaggcggcc aagcccaaga aggcggccat ctcctcgaag aagaactccg 3301 ccaagaagaa tgcctcctcc accaaggcat agaaggagta gatctccagt aagacgaaga 3361 agacgttcgt cagcatcctt gtctgggagt agctcatcat cctcttcatc tcgttcacgg 3421 tcaccaccaa agaagcctcc caagaggaca tccagccccc ctcggaaaac tcgtaggtta 3481 tctccttcag caagtcctcc aaggcgaagg cacaggccat cacctcctgc aactccacca 3541 cccaaaactc ggcattcccc tacaccccag cagtcaaacc gtacaagaaa aagtcgtgtt 3601 tctgtgtctc cagggagaac ttcagtgaca aaacataaag gtactgagaa aagagaatcc 3661 ccttcaccag caccgaagcc tagaaaagta gagttatctg aatcggaaga agataaaggt 3721 ggcaaaatgg ctgcagcaga ttctgtgcag cagagacgcc aatacagacg acaaaaccag 3781 cagtcttcat ctgactctgg ctcctcctcc tcctcagaag atgaacgacc caagagatcc 3841 catgtgaaga atggtgaggt tggcaggcgg cggagacatt ccccttcccg gagtgcttct 3901 ccatcaccac gaaagcgcca aaaagagact tcccctcgga tgcagatggg aaagcgatgg 3961 caatcgccag tgactaaaag tggtagacgg aggagaagtc catccccacc acccaccaga 4021 aggcgacggt ctccttctcc cgcccctcct cctcgacggc gcaggactcc cacaccacca 4081 ccacgacgaa ggactccttc tcctccccca cgtcggcgct caccttctcc tagaagatac 4141 tctcctccaa tacagaggag atactctcct tctccacctc caaagagaag aacggcttca 4201 cctcctcccc ctcctaaacg aagagcatca ccatctccac caccaaagcg gcgggtctcc 4261 cattctccac ctcccaaaca aagaagctcc ccagtcacca agagacgttc accttcatta 4321 tcatccaagc ataggaaagg gtcttcccca agccgctcta cccgggaggc ccgatcacca 4381 caaccaaaca aacggcattc gccctcacca cggcctcgag ctcctcagac ctcctcaagt 4441 cctccacccg ttcgaagagg agcgtcgtca tcaccccaaa gaaggcagtc cccgtctcca 4501 agtactaggc ccattaggag agtctccagg actccggaac ctaaaaagat aaaaaaggct 4561 gcttccccaa gcccacagtc tgtaagaagg gtctcatcct cccgatctgt ctccgggtct 4621 cctgagccag cagctaaaaa gcccccagca cctccatccc ccgtccagtc tcagtcaccg 4681 tctacaaact ggtcaccagc tgtaccggtc aaaaaggcca aaagcccaac accgagccca 4741 tcaccgccaa gaaattcaga tcaggaagga ggtggaaaga aaaagaagaa aaagaaggac 4801 aagaaacaca aaaaggataa gaagcacaag aagcacaaaa aacacaagaa ggaaaaggct 4861 gtggctgcag ctgctgcagc tgctgtgacc cctgcagcca ttgcagctgc cacaaccaca 4921 ttagcacagg aagagccagt ggcagcgcca gagccgaaga aggagactga aagtgaagct 4981 gaagataacc ttgatgattt agaaaagcac ctgcgtgaaa aggccctgag atcaatgagg 5041 aaggcccaag tgtccccaca gtctggatcc GACTACAAAG ACCATGACGG TGATTATAAA 5101 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 5161 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 5221 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 5281 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 5341 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 5401 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 5461 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 5521 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 5581 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 5641 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 5701 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 5761 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 5821 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 5881 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 5941 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 6001 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 6061 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 6121 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 6181 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 6241 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 6301 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 6361 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 6421 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 6481 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 6541 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 6601 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 6661 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 6721 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 6781 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 6841 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 6901 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 6961 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 7021 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 7081 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 7141 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 7201 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 7261 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 7321 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 7381 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 7441 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 7501 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 7561 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 7621 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 7681 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 7741 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 7801 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 7861 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 7921 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 7981 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 8041 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 8101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 8161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 8221 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 8281 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 8341 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 8401 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 8461 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 8521 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 8581 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 8641 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 8701 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 8761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 8821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 8881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 8941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 9001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 9061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 9121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 9181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 9241 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 9301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 9361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 9421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 9481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 9541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 9601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 9661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 9721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 9781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 9841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 9901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 9961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 10021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 10081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 10141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 10201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 10261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 10321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 10381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 10441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 10501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 10561 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 10621 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 10681 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 10741 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 10801 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 10861 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 10921 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 10981 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???