pLV3-CMV-TLR2-8×His-CopGFP-Puro plasmid

PVT99407

pLV3-CMV-TLR2-8×His-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TLR2(human)-8×His-CopGFP-Puro
Alias: NM_001318787.2;CD282; TIL4
Gene lenght: 2352bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TLR2-8×His-CopGFP-Puro plasmid Description

pLV3-CMV-TLR2-8×His-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TLR2-8×His-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc cacatacttt gtggatggtg 2341 tgggtcttgg gggtcatcat cagcctctcc aaggaagaat cctccaatca ggcttctctg 2401 tcttgtgacc gcaatggtat ctgcaagggc agctcaggat ctttaaactc cattccctca 2461 gggctcacag aagctgtaaa aagccttgac ctgtccaaca acaggatcac ctacattagc 2521 aacagtgacc tacagaggtg tgtgaacctc caggctctgg tgctgacatc caatggaatt 2581 aacacaatag aggaagattc tttttcttcc ctgggcagtc ttgaacattt agacttatcc 2641 tataattact tatctaattt atcgtcttcc tggttcaagc ccctttcttc tttaacattc 2701 ttaaacttac tgggaaatcc ttacaaaacc ctaggggaaa catctctttt ttctcatctc 2761 acaaaattgc aaatcctgag agtgggaaat atggacacct tcactaagat tcaaagaaaa 2821 gattttgctg gacttacctt ccttgaggaa cttgagattg atgcttcaga tctacagagc 2881 tatgagccaa aaagtttgaa gtcaattcag aatgtaagtc atctgatcct tcatatgaag 2941 cagcatattt tactgctgga gatttttgta gatgttacaa gttccgtgga atgtttggaa 3001 ctgcgagata ctgatttgga cactttccat ttttcagaac tatccactgg tgaaacaaat 3061 tcattgatta aaaagtttac atttagaaat gtgaaaatca ccgatgaaag tttgtttcag 3121 gttatgaaac ttttgaatca gatttctgga ttgttagaat tagagtttga tgactgtacc 3181 cttaatggag ttggtaattt tagagcatct gataatgaca gagttataga tccaggtaaa 3241 gtggaaacgt taacaatccg gaggctgcat attccaaggt tttacttatt ttatgatctg 3301 agcactttat attcacttac agaaagagtt aaaagaatca cagtagaaaa cagtaaagtt 3361 tttctggttc cttgtttact ttcacaacat ttaaaatcat tagaatactt ggatctcagt 3421 gaaaatttga tggttgaaga atacttgaaa aattcagcct gtgaggatgc ctggccctct 3481 ctacaaactt taattttaag gcaaaatcat ttggcatcat tggaaaaaac cggagagact 3541 ttgctcactc tgaaaaactt gactaacatt gatatcagta agaatagttt tcattctatg 3601 cctgaaactt gtcagtggcc agaaaagatg aaatatttga acttatccag cacacgaata 3661 cacagtgtaa caggctgcat tcccaagaca ctggaaattt tagatgttag caacaacaat 3721 ctcaatttat tttctttgaa tttgccgcaa ctcaaagaac tttatatttc cagaaataag 3781 ttgatgactc taccagatgc ctccctctta cccatgttac tagtattgaa aatcagtagg 3841 aatgcaataa ctacgttttc taaggagcaa cttgactcat ttcacacact gaagactttg 3901 gaagctggtg gcaataactt catttgctcc tgtgaattcc tctccttcac tcaggagcag 3961 caagcactgg ccaaagtctt gattgattgg ccagcaaatt acctgtgtga ctctccatcc 4021 catgtgcgtg gccagcaggt tcaggatgtc cgcctctcgg tgtcggaatg tcacaggaca 4081 gcactggtgt ctggcatgtg ctgtgctctg ttcctgctga tcctgctcac gggggtcctg 4141 tgccaccgtt tccatggcct gtggtatatg aaaatgatgt gggcctggct ccaggccaaa 4201 aggaagccca ggaaagctcc cagcaggaac atctgctatg atgcatttgt ttcttacagt 4261 gagcgggatg cctactgggt ggagaacctt atggtccagg agctggagaa cttcaatccc 4321 cccttcaagt tgtgtcttca taagcgggac ttcattcctg gcaagtggat cattgacaat 4381 atcattgact ccattgaaaa gagccacaaa actgtctttg tgctttctga aaactttgtg 4441 aagagtgagt ggtgcaagta tgaactggac ttctcccatt tccgtctttt tgatgagaac 4501 aatgatgctg ccattctcat tcttctggag cccattgaga aaaaagccat tccccagcgc 4561 ttctgcaagc tgcggaagat aatgaacacc aagacctacc tggagtggcc catggacgag 4621 gctcagcggg aaggattttg ggtaaatctg agagctgcga taaagtccgg atccCACCAT 4681 CACCATCACC ATCACCATta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 4741 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4801 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4861 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4921 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4981 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 5041 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 5101 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 5161 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 5221 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 5281 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 5341 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 5401 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 5461 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 5521 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 5581 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 5641 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 5701 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 5761 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 5821 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 5881 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 5941 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 6001 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 6061 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6121 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 6181 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 6241 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 6301 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 6361 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 6421 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 6481 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 6541 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 6601 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6661 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 7021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 7081 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 7141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 7201 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 7261 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7321 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 7381 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7441 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7501 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7561 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7621 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7681 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7741 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 7801 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 7861 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 7921 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 7981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 8041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 8101 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 8161 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8521 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 8581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8761 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 8821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 9001 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 9061 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 9121 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9181 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9241 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9301 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9361 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9421 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9481 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9541 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9601 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9661 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9721 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9781 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9841 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9901 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9961 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 10021 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 10081 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 10141 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 10201 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 10261 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 10321 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 10381 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 10441 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 10501 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 10561 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???