pLV3-CMV-TMTC3-3×FLAG-CopGFP-Puro plasmid

PVT111317

pLV3-CMV-TMTC3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TMTC3(human)-3×FLAG-CopGFP-Puro
Alias: NM_181783.4;LIS8; SMILE
Gene lenght: 2742
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TMTC3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TMTC3-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-TMTC3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ctaatattaa cctaaaagaa 2341 ataaccttaa tagtaggtgt ggttactgcc tgctattgga acagcctctt ttgtggtttt 2401 gtttttgatg atgtttcagc aatactggat aacaaagact tgcatccatc tacaccttta 2461 aaaactttat ttcaaaatga cttctgggga acccctatgt ctgaggagag aagccacaag 2521 tcttaccgtc ccttaacagt attgacattt cgcttaaatt atttgttaag tgaactaaaa 2581 ccaatgtcat atcatctcct gaatatgatt tttcatgctg tggttagtgt gatatttctc 2641 aaagtatgca aactttttct ggacaacaag agtagtgtga ttgcttcttt actttttgca 2701 gtgcacccaa tacacacaga agcagtaaca ggagttgttg gaagagcaga acttttgtca 2761 tctatctttt ttctagcagc ttttttgtca tataccagat caaaaggacc agacaattcc 2821 ataatatgga ctccaattgc cttgacagtg tttttagtgg ctgttgcaac attatgtaaa 2881 gaacaaggaa taacagttgt aggaatttgc tgtgtgtatg aagtgtttat tgcccagggg 2941 tatactttgc cattactatg tactactgct ggacagtttc tccgtggaaa gggtagcatt 3001 ccattttcta tgctgcagac actagtaaaa ctcattgtct tgatgttcag tacattatta 3061 cttgttgtga ttagagtcca ggttattcaa tcccaacttc cagtattcac caggtttgat 3121 aacccagctg ctgtaagccc aactcctaca aggcaactaa cttttaacta cctccttcct 3181 gtgaatgctt ggttgttatt aaatccttca gagctctgct gtgattggac catgggaaca 3241 ataccactta tagagtcatt actagatatt cgaaatctgg ccacatttac tttcttttgt 3301 tttctgggga tgttgggagt attcagtatc agatactctg gtgattcctc caagactgtt 3361 ttaatggcgc tttgtttaat ggcattacca tttattcctg catcgaacct tttttttcca 3421 gttggatttg ttgttgccga gcgagtatta tatgttccca gcatggggtt ctgtattttg 3481 gtagcccatg gatggcagaa aatatcaaca aaaagtgtat ttaaaaagct atcctggatt 3541 tgtctgtcta tggtgatact cactcattcc ttaaaaacat tccacagaaa ttgggattgg 3601 gagtctgaat atacattgtt tatgtcagcc ttgaaggtaa ataaaaataa tgccaaactt 3661 tggaataatg tgggtcatgc tctggaaaat gaaaagaact ttgagagagc tttgaaatac 3721 ttcttacagg ctacccatgt tcagccagat gatattggtg cccatatgaa tgtaggaaga 3781 acttataaaa atttaaatag aaccaaagaa gctgaagaat cttacatgat ggctaaatca 3841 ctgatgcctc aaattattcc tggtaaaaaa tatgcagcca gaattgcccc taaccaccta 3901 aatgtttata tcaatctggc taacctgatc cgagcaaatg agtcccgact ggaagaagca 3961 gatcagctgt accgtcaagc aataagcatg aggcccgact tcaagcaggc ttacattagc 4021 agaggagaat tgcttttaaa aatgaataaa cctcttaaag caaaggaagc atatcttaaa 4081 gcactagagc tggacagaaa taatgcagat ctttggtaca acttggcaat tgtacatatt 4141 gaacttaaag aaccaaatga agccctaaaa aactttaatc gtgctctgga actaaatcca 4201 aagcataaac tagcattatt caactctgct atagtaatgc aagaatcagg tgaggttaaa 4261 ctcagacctg aagctagaaa acgacttcta agttatataa atgaagagcc actagatgct 4321 aatgggtatt tcaatttggg aatgcttgcc atggatgaca aaaaggacaa tgaagcagag 4381 atttggatga agaaagccat aaagttacaa gccgacttcc gaagtgcttt gtttaatctg 4441 gctctcctgt attcccagac tgcaaaggaa ttaaaggctt tgccaatttt ggaggagtta 4501 ctcagatact accctgatca tatcaagggc ctcattttaa aaggagacat tctgatgaat 4561 caaaagaaag atatactagg agcaaaaaaa tgttttgaaa ggattttgga gatggatcca 4621 agcaatgtgc aaggaaaaca caatctttgt gttgtttatt ttgaagaaaa agacttatta 4681 aaagctgaaa gatgccttct tgaaacactg gcattagcac cacatgaaga atatattcag 4741 cgccatttga atatagtcag ggataagatt tcctcatcta gttttataga gccaatattc 4801 ccaaccagta agatttcaag tgtggaagga aagaaaattc caactgaaag tgtaaaagaa 4861 attagaggtg aatccagaca aacacaaata gtaaaaacaa gtgataataa aagtcagtct 4921 aaatccaaca aacaattagg aaaaaatgga gacgaagaga caccccacaa aacaacaaaa 4981 gacatcaaag aaattgagaa gaaaagagtt gctgctttaa aaagactaga agagattgaa 5041 cgtattttaa atggtgaagg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 5101 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 5161 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 5221 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 5281 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 5341 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 5401 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 5461 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 5521 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 5581 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 5641 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 5701 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 5761 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 5821 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 5881 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 5941 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 6001 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 6061 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 6121 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 6181 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 6241 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 6301 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 6361 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 6421 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 6481 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 6541 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 6601 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 6661 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 6721 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 6781 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 6841 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 6901 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 6961 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 7021 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 7081 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 7141 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 7201 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 7261 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 7321 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 7381 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 7441 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 7501 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 7561 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7621 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 7681 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 7741 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 7801 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7861 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7921 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7981 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 8041 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 8101 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 8161 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 8221 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 8281 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 8341 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 8401 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 8461 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 8521 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 8581 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 8641 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8701 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8761 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8821 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8881 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8941 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 9001 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 9061 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 9121 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 9181 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 9241 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 9301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 9361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9421 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 9481 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 9541 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 9601 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 9661 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9721 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9781 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9841 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 9901 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9961 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 10021 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 10081 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 10141 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 10201 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 10261 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 10321 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 10381 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 10441 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 10501 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 10561 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 10621 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 10681 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10741 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 10801 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 10861 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 10921 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 10981 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.