pLV3-CMV-TOP2B-3×HA-T2A-mCherry-Fluc-Puro plasmid

PVT97667

pLV3-CMV-TOP2B-3×HA-T2A-mCherry-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-TOP2B(human)-3×HA-T2A-mCherry-Fluc-Puro
Alias: NM_001330700.2;BILU; top2beta; TOPIIB
Gene lenght: 4878bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red,Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-TOP2B-3×HA-T2A-mCherry-Fluc-Puro plasmid Description

pLV3-CMV-TOP2B-3×HA-T2A-mCherry-Fluc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TOP2B-3×HA-T2A-mCherry-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catggccaag tcgggtggct gcggcgcggg agccggcgtg 2341 ggcggcggca acggggcact gacctgggtg actctttttg atcagaacaa tgctgcaaaa 2401 aaagaagagt cagaaactgc caacaaaaat gattcttcaa agaagttgtc tgttgagaga 2461 gtgtatcaga agaagacaca acttgaacac attcttcttc gtcctgatac atatattggg 2521 tcagtggagc cattgacgca gttcatgtgg gtgtatgatg aagatgtagg aatgaattgc 2581 agggaggtta cctttgtgcc aggtttatac aagatctttg atgaaatttt ggttaatgct 2641 gctgacaata aacagaggga taagaacatg acttgtatta aagtttctat tgatcctgaa 2701 tctaacatta taagcatttg gaataatggg aaaggcattc cagtagtaga acacaaggta 2761 gagaaagttt atgttcctgc tttaattttt ggacagcttt taacatccag taactatgat 2821 gatgatgaga aaaaagttac aggtggtcgt aatggttatg gtgcaaaact ttgtaatatt 2881 ttcagtacaa agtttacagt agaaacagct tgcaaagaat acaaacacag ttttaagcag 2941 acatggatga ataatatgat gaagacttct gaagccaaaa ttaaacattt tgatggtgaa 3001 gattacacat gcataacatt ccaaccagat ctgtccaaat ttaagatgga aaaacttgac 3061 aaggatattg tggccctcat gactagaagg gcatatgatt tggctggttc gtgtagaggg 3121 gtcaaggtca tgtttaatgg aaagaaattg cctgtaaatg gatttcgcag ttatgtagat 3181 ctttatgtga aagacaaatt ggatgaaact ggggtggccc tgaaagttat tcatgagctt 3241 gcaaatgaaa gatgggatgt ttgtctcaca ttgagtgaaa aaggattcca gcaaatcagc 3301 tttgtaaata gtattgcaac tacaaaaggt ggacggcacg tggattatgt ggtagatcaa 3361 gttgttggta aactgattga agtagttaag aaaaagaaca aagctggtgt atcagtgaaa 3421 ccatttcaag taaaaaacca tatatgggtt tttattaatt gccttattga aaatccaact 3481 tttgattctc agactaagga aaacatgact ctgcagccca aaagttttgg gtctaaatgc 3541 cagctgtcag aaaaattttt taaagcagcc tctaattgtg gcattgtaga aagtatcctg 3601 aactgggtga aatttaaggc tcagactcag ctgaataaga agtgttcatc agtaaaatac 3661 agtaaaatca aaggtattcc caaactggat gatgctaatg atgctggtgg taaacattcc 3721 ctggagtgta cactgatatt aacagaggga gactctgcca aatcactggc tgtgtctgga 3781 ttaggtgtga ttggacgaga cagatacgga gtttttccac tcaggggcaa aattcttaat 3841 gtacgggaag cttctcataa acagatcatg gaaaatgctg aaataaataa tattattaaa 3901 atagttggtc tacaatataa gaaaagttac gatgatgcag aatctctgaa aaccttacgc 3961 tatggaaaga ttatgattat gaccgatcag gatcaagatg gttctcacat aaaaggcctg 4021 cttattaatt tcatccatca caattggcca tcacttttga agcatggttt tcttgaagag 4081 ttcattactc ctattgtaaa ggcaagcaaa aataagcagg aactttcctt ctacagtatt 4141 cctgaatttg acgaatggaa aaaacatata gaaaaccaga aagcctggaa aataaagtac 4201 tataaaggat tgggtactag tacagctaaa gaagcaaagg aatattttgc tgatatggaa 4261 aggcatcgca tcttgtttag atatgctggt cctgaagatg atgctgccat taccttggca 4321 tttagtaaga agaagattga tgacagaaaa gaatggttaa caaattttat ggaagaccgg 4381 agacagcgta ggctacatgg cttaccagag caatttttat atggtactgc aacaaagcat 4441 ttgacttata atgatttcat caacaaggaa ttgattctct tctcaaactc agacaatgaa 4501 agatctatac catctcttgt tgatggcttt aaacctggcc agcggaaagt tttatttacc 4561 tgtttcaaga ggaatgataa acgtgaagta aaagttgccc agttggctgg ctctgttgct 4621 gagatgtcgg cttatcatca tggagaacaa gcattgatga tgactattgt gaatttggct 4681 cagaactttg tgggaagtaa caacattaac ttgcttcagc ctattggtca gtttggaact 4741 cggcttcatg gtggcaaaga tgctgcaagc cctcgttata ttttcacaat gttaagcact 4801 ttagcaaggc tactttttcc tgctgtggat gacaacctcc ttaagttcct ttatgatgat 4861 aatcaacgtg tagagcctga gtggtatatt cctataattc ccatggtttt aataaatggt 4921 gctgagggca ttggtactgg atgggcttgt aaactaccca actatgatgc tagggaaatt 4981 gtgaacaatg tcagacgaat gctagatggc ctggatcctc atcccatgct tccaaactac 5041 aaaaacttta aaggcacgat tcaagaactt ggtcaaaacc agtatgcagt cagtggtgaa 5101 atatttgtag tggacagaaa cacagtagaa attacagagc ttccagttag aacttggaca 5161 caggtatata aagaacaggt tttagaacct atgctaaatg gaacagataa aacaccagca 5221 ttaatttctg attataaaga atatcatact gacacaactg tgaaatttgt ggtgaaaatg 5281 actgaagaga aactagcaca agcagaagct gctggactgc ataaagtttt taaacttcaa 5341 actactctta cttgtaattc catggtactt tttgatcata tgggatgtct gaagaaatat 5401 gaaactgtgc aagacattct gaaagaattc tttgatttac gattaagtta ttacggttta 5461 cgtaaggagt ggcttgtggg aatgttggga gcagaatcta caaagcttaa caatcaagcc 5521 cgtttcattt tagagaagat acaagggaaa attactatag agaataggtc aaagaaagat 5581 ttgattcaaa tgttagtcca gagaggttat gaatctgacc cagtgaaagc ctggaaagaa 5641 gcacaagaaa aggcagcaga agaggatgaa acacaaaacc agcatgatga tagttcctcc 5701 gattcaggaa ctccttcagg cccagatttt aattatattt taaatatgtc tctgtggtct 5761 cttactaaag aaaaagttga agaactgatt aaacagagag atgcaaaagg gcgagaggtc 5821 aatgatctta aaagaaaatc tccttcagat ctttggaaag aggatttagc ggcatttgtt 5881 gaagaactgg ataaagtgga atctcaagaa cgagaagatg ttctggctgg aatgtctgga 5941 aaagcaatta aaggtaaagt tggcaaacct aaggtgaaga aactccagtt ggaagagaca 6001 atgccctcac cttatggcag aagaataatt cctgaaatta cagctatgaa ggcagatgcc 6061 agcaaaaagt tgctgaagaa gaagaagggt gatcttgata ctgcagcagt aaaagtggaa 6121 tttgatgaag aattcagtgg agcaccagta gaaggtgcag gagaagaggc attgactcca 6181 tcagttccta taaataaagg tcccaaacct aagagggaga agaaggagcc tggtaccaga 6241 gtgagaaaaa cacctacatc atctggtaaa cctagtgcaa agaaagtgaa gaaacggaat 6301 ccttggtcag atgatgaatc caagtcagaa agtgatttgg aagaaacaga acctgtggtt 6361 attccaagag attctttgct taggagagca gcagccgaaa gacctaaata cacatttgat 6421 ttctcagaag aagaggatga tgatgctgat gatgatgatg atgacaataa tgatttagag 6481 gaattgaaag ttaaagcatc tcccataaca aatgatgggg aagatgaatt tgttccttca 6541 gatgggttag ataaagatga atatacattt tcaccaggca aatcaaaagc cactccagaa 6601 aaatctttgc atgacaaaaa aagtcaggat tttggaaatc tcttctcatt tccttcatat 6661 tctcagaagt cagaagatga ttcagctaaa tttgacagta atgaagaaga ttctgcttct 6721 gttttttcac catcatttgg tctgaaacag acagataaag ttccaagtaa aacggtagct 6781 gctaaaaagg gaaaaccgtc ttcagataca gtccctaagc ccaagagagc cccaaaacag 6841 aagaaagtag tagaggctgt aaactctgac tcggattcag aatttggcat tccaaagaag 6901 actacaacac caaaaggtaa aggccgaggg gcaaagaaaa ggaaagcatc tggctctgaa 6961 aatgaaggcg attataaccc tggcaggaaa acatccaaaa caacaagcaa gaaaccgaag 7021 aagacatctt ttgatcagga ttcagatgtg gacatcttcc cctcagactt ccctactgag 7081 ccaccttctc tgccacgaac cggtcgggct aggaaagaag taaaatattt tgcagagtct 7141 gatgaagaag aagatgatgt tgattttgca atgtttaatT CTAGATACCC ATACGACGTA 7201 CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC 7261 CCAGACTACG CAGGATCCga gggcagagga agtcttctaa catgcggtga cgtggaggag 7321 aatcccggcc cttccggaat ggagagcgac gagagcggcc tggtgagcaa gggcgaggag 7381 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg 7441 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 7501 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc 7561 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 7621 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 7681 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg 7741 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc 7801 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag 7861 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag 7921 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc 7981 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc 8041 accggcggca tggacgagct gtacaagtag ggatccgcgg ccgcaaggat ctgcgatcgc 8101 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 8161 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 8221 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 8281 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 8341 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 8401 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 8461 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 8521 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 8581 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 8641 accatggaga gcgacgagag cggcctgatg gaagacgcca aaaacataaa gaaaggcccg 8701 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag 8761 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc 8821 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg 8881 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg 8941 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa 9001 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag 9061 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc 9121 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat 9181 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca 9241 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct 9301 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc 9361 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact 9421 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag 9481 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta 9541 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa 9601 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc 9661 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt 9721 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg 9781 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt 9841 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg 9901 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac 9961 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc 10021 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt 10081 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag 10141 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag 10201 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac 10261 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgaga 10321 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 10381 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 10441 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 10501 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 10561 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 10621 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 10681 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 10741 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 10801 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 10861 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 10921 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 10981 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 11041 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 11101 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 11161 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 11221 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 11281 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 11341 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 11401 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 11461 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 11521 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 11581 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 11641 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 11701 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 11761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 11821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 11881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 11941 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 12001 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 12061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 12121 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 12181 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 12241 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 12301 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 12361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 12421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 12481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 12541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 12601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 12661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 12721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 12781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 12841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 12901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 12961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 13021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 13081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 13141 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 13201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 13261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 13321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 13381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 13441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 13501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 13561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 13621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 13681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 13741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 13801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 13861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 13921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 13981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 14041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 14101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 14161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 14221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 14281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 14341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 14401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 14461 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 14521 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 14581 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 14641 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 14701 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 14761 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 14821 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 14881 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 14941 agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???