pLV3-CMV-TP53-3×FLAG-mCherry-Puro plasmid

PVT100517

pLV3-CMV-TP53-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-TP53(human)-3×FLAG-mCherry-Puro
Alias: NM_000546.6;BCC7; BMFS5; LFS1; P53; TRP53
Gene lenght: 1182bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-TP53-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-TP53-3×FLAG-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TP53-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggagga gccgcagtca gatcctagcg tcgagccccc 2341 tctgagtcag gaaacatttt cagacctatg gaaactactt cctgaaaaca acgttctgtc 2401 ccccttgccg tcccaagcaa tggatgattt gatgctgtcc ccggacgata ttgaacaatg 2461 gttcactgaa gacccaggtc cagatgaagc tcccagaatg ccagaggctg ctccccccgt 2521 ggcccctgca ccagcagctc ctacaccggc ggcccctgca ccagccccct cctggcccct 2581 gtcatcttct gtcccttccc agaaaaccta ccagggcagc tacggtttcc gtctgggctt 2641 cttgcattct gggacagcca agtctgtgac ttgcacgtac tcccctgccc tcaacaagat 2701 gttttgccaa ctggccaaga cctgccctgt gcagctgtgg gttgattcca cacccccgcc 2761 cggcacccgc gtccgcgcca tggccatcta caagcagtca cagcacatga cggaggttgt 2821 gaggcgctgc ccccaccatg agcgctgctc agatagcgat ggtctggccc ctcctcagca 2881 tcttatccga gtggaaggaa atttgcgtgt ggagtatttg gatgacagaa acacttttcg 2941 acatagtgtg gtggtgccct atgagccgcc tgaggttggc tctgactgta ccaccatcca 3001 ctacaactac atgtgtaaca gttcctgcat gggcggcatg aaccggaggc ccatcctcac 3061 catcatcaca ctggaagact ccagtggtaa tctactggga cggaacagct ttgaggtgcg 3121 tgtttgtgcc tgtcctggga gagaccggcg cacagaggaa gagaatctcc gcaagaaagg 3181 ggagcctcac cacgagctgc ccccagggag cactaagcga gcactgccca acaacaccag 3241 ctcctctccc cagccaaaga agaaaccact ggatggagaa tatttcaccc ttcagatccg 3301 tgggcgtgag cgcttcgaga tgttccgaga gctgaatgag gccttggaac tcaaggatgc 3361 ccaggctggg aaggagccag gggggagcag ggctcactcc agccacctga agtccaaaaa 3421 gggtcagtct acctcccgcc ataaaaaact catgttcaag acagaagggc ctgactcaga 3481 cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 3541 TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc 3601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 3661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 3721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 3781 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 3841 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 3901 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 3961 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 4021 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 4081 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga 4141 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 4201 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 4261 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 4321 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 4381 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 4441 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 4501 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 4561 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 4621 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 4681 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 4741 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 4801 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct 4861 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 4921 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 4981 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 5041 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 5101 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 5161 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5221 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 5281 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 5341 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 5401 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 5461 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5881 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6001 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 6061 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6121 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6181 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6241 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6301 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6361 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6421 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6481 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6541 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6661 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6721 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 6781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 6841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 6901 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 6961 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 7021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 7261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 7321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 7381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 7441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 7501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 7561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 7621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 7681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 7741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 7801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 7861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 7921 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 7981 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 8041 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 8101 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 8161 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 8221 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 8281 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 8341 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 8401 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 8461 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 8521 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 8581 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 8641 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 8701 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 8761 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 8821 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 8881 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 8941 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 9001 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 9061 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 9121 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 9181 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 9241 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 9301 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 9361 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???