pLV3-CMV-UBE2L3-3×HA-EF1a-mCherry-Puro plasmid

PVT94146

pLV3-CMV-UBE2L3-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-UBE2L3(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_003347.4;E2-F1; L-UBC; UBCH7; UbcM4
Gene lenght: 462bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBE2L3-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-UBE2L3-3×HA-EF1a-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-UBE2L3-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcggc cagcaggagg ctgatgaagg agcttgaaga 2341 aatccgcaaa tgtgggatga aaaacttccg taacatccag gttgatgaag ctaatttatt 2401 gacttggcaa gggcttattg ttcctgacaa ccctccatat gataagggag ccttcagaat 2461 cgaaatcaac tttccagcag agtacccatt caaaccaccg aagatcacat ttaaaacaaa 2521 gatctatcac ccaaacatcg acgaaaaggg gcaggtctgt ctgccagtaa ttagtgccga 2581 aaactggaag ccagcaacca aaaccgacca agtaatccag tccctcatag cactggtgaa 2641 tgacccccag cctgagcacc cgcttcgggc tgacctagct gaagaatact ctaaggaccg 2701 taaaaaattc tgtaagaatg ctgaagagtt tacaaagaaa tatggggaaa agcgacctgt 2761 ggacggatcc TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA 2821 TTATGCTAGC TATCCTTATG ATGTCCCAGA CTACGCATAG gcggccgcGa aggatctgcg 2881 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2941 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa 3001 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 3061 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc 3121 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac 3181 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg 3241 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc 3301 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct 3361 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag 3421 acgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 3481 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 3541 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 3601 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 3661 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 3721 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 3781 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 3841 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 3901 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 3961 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 4021 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 4081 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat 4141 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 4201 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 4261 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 4321 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 4381 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 4441 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 4501 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 4561 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 4621 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 4681 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 4741 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 4801 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4861 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4921 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4981 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5041 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 5101 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 5161 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 5221 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5281 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 5341 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 5401 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 5461 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 5521 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 5581 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 5641 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 5701 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 5761 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5881 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 5941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6061 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6901 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8221 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8281 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8341 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8401 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8461 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 8521 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 8581 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 8641 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 8701 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???