pLV3-CMV-UBE2NL-3×FLAG-CopGFP-Puro plasmid

PVT109917

pLV3-CMV-UBE2NL-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UBE2NL(human)-3×FLAG-CopGFP-Puro
Alias: NM_001012989.3;Li174
Gene lenght: 459
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBE2NL-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-UBE2NL-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-UBE2NL-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccgagctgcc ccacaggatc 2341 atcaaggaaa cccagcgttt gctggcagag ccagttcctg gcatcaaagc agaaccagat 2401 gaaagcaacg cccgttattt tcatgtggtc attgctgggg aatcaaagga ttcccccttt 2461 gagggaggga cttttaaacg tgaactatta cttgcagaag aatacccaat ggcagcccct 2521 aaagtacgtt tcatgaccaa aatttatcat ccaaatgtag acaagttgga aagaataagt 2581 ttagatattt tgaaagataa gtggtcccca gccctgcaga tccgcacagt tctgctatcg 2641 atccaggcct tgttaaatgc tcccaatcca gatgatccat tagcaaatga tgtagtggag 2701 cagtggaaga ccaacgaagc ccaagccatt gaaacagcta gagcatggac taggctatat 2761 gccatgaata gtattggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 2821 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 2881 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 2941 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3001 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3061 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3121 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3181 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3241 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3301 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 3361 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 3421 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 3481 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 3541 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 3601 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 3661 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 3721 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 3781 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 3841 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 3901 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 3961 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4021 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4081 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4141 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4201 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4261 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4321 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4381 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4441 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4501 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 4561 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 4621 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 4681 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 4741 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 4801 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 4861 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 4921 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 4981 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5041 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5101 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5161 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5221 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5281 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5341 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 5401 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 5461 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 5521 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 5581 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 5641 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 5701 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 5761 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 5821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5881 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 5941 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6061 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 6121 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6241 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6301 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6361 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 6421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 6481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 6541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 6601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 6661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 6721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 6781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 6841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 6901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 6961 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7261 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7321 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7381 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 7441 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 7501 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 7561 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 7621 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 7681 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 7741 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 7801 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7861 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 7921 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 7981 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8041 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8101 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8161 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8281 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8341 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 8401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 8461 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 8521 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 8581 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 8641 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 8701 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.