pLV3-CMV-UBE2Q2-3×FLAG-CopGFP-Puro plasmid

PVT109571

pLV3-CMV-UBE2Q2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UBE2Q2(human)-3×FLAG-CopGFP-Puro
Alias: NM_173469.4
Gene lenght: 1125
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBE2Q2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-UBE2Q2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-UBE2Q2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt ccgtgtcagg gctcaaggcc 2341 gagctgaagt tcctggcgtc catcttcgac aagaaccacg agcgattccg catcgtcagt 2401 tggaagctgg acgagctgca ctgccagttc ctggtgccgc agcagggcag cccgcactcg 2461 ctgccgccgc cactcacgct ccactgcaac atcacggaat cctatccatc ttcttcaccg 2521 atatggtttg tggattctga agacccaaat ctgacatcag ttctggaacg tctagaagat 2581 actaagaaca acaatttgct tcgtcagcaa ttgaagtggt tgatatgtga actctgcagt 2641 ttatataacc ttcctaagca cctggatgtt gagatgctag atcaaccact acccacgggt 2701 cagaatggga caacagaaga agtgacttca gaagaagagg aagaagaaga agagatggct 2761 gaagatatag aagacttaga tcactatgag atgaaggaag aagagcctat tagtgggaaa 2821 aagtcagagg atgaaggaat tgaaaaagaa aatttggcaa tattagagaa aattaggaag 2881 actcaaaggc aagaccattt aaatggtgca gtgtctgggt cagtgcaagc ttcagataga 2941 cttatgaaag agctcaggga catatacaga tcacagagtt ataaaacagg gatttattca 3001 gtggaactca taaatgacag tttatatgac tggcatgtta aactgcagaa ggttgaccct 3061 gatagtcctt tgcacagtga tcttcagatc ttaaaagaaa aagaaggcat agaatatatt 3121 ttgcttaact tctcttttaa ggataacttt ccatttgatc ctccatttgt tcgagtggtg 3181 ttacctgttc tctcaggagg gtatgtattg ggtggaggag cattatgtat ggaacttctc 3241 acaaaacagg gctggagcag tgcctactca atagaatcgg tcatcatgca aataaatgcc 3301 accttagtca aaggcaaagc cagagtgcag tttggagcaa ataagaatca atataatcta 3361 gcaagagccc aacaatccta taattccatt gtacagatac atgagaaaaa tggctggtac 3421 acccctccaa aggaagatgg cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3481 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3541 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3601 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3661 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3721 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3781 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3841 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3901 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3961 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4021 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4081 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4141 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4201 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4261 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4321 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4381 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4441 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4501 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4561 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4621 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 4681 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4741 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 4801 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 4861 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 4921 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4981 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5041 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5101 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5161 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5221 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5281 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5341 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5401 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5461 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5521 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5581 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5641 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5701 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5761 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5821 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5881 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 5941 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6001 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6061 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6121 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6181 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6241 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6301 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6361 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6421 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6481 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6541 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6601 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 6661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6721 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 6781 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6841 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6961 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7381 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7621 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7921 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7981 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8041 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8101 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8161 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8221 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8281 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8341 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8401 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8461 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8521 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8581 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8641 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8701 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8761 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8881 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8941 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9001 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9061 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9121 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9181 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9241 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9301 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9361 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.