pLV3-CMV-VEGFA-3×FLAG-CopGFP-Puro plasmid

PVT111748

pLV3-CMV-VEGFA-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-VEGFA(human)-3×FLAG-CopGFP-Puro
Alias: NM_001025366.2;MVCD1; VEGF; VPF
Gene lenght: 1236
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F（special construction）
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-VEGFA-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-VEGFA-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-VEGFA-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccAtga cggacagaca gacagacacc 2341 gcccccagcc ccagctacca cctcctcccc ggccggcggc ggacagtgga cgcggcggcg 2401 agccgcgggc aggggccgga gcccgcgccc ggaggcgggg tggagggggt cggggctcgc 2461 ggcgtcgcac tgaaactttt cgtccaactt ctgggctgtt ctcgcttcgg aggagccgtg 2521 gtccgcgcgg gggaagccga gccgagcgga gccgcgagaa gtgctagctc gggccgggag 2581 gagccgcagc cggaggaggg ggaggaggaa gaagagaagg aagaggagag ggggccgcag 2641 tggcgactcg gcgctcggaa gccgggctca tggacgggtg aggcggcggt gtgcgcagac 2701 agtgctccag ccgcgcgcgc tccccaggcc ctggcccggg cctcgggccg gggaggaaga 2761 gtagctcgcc gaggcgccga ggagagcggg ccgccccaca gcccgagccg gagagggagc 2821 gcgagccgcg ccggccccgg tcgggcctcc gaaaccatga actttctgct gtcttgggtg 2881 cattggagcc ttgccttgct gctctacctc caccatgcca agtggtccca ggctgcaccc 2941 atggcagaag gaggagggca gaatcatcac gaagtggtga agttcatgga tgtctatcag 3001 cgcagctact gccatccaat cgagaccctg gtggacatct tccaggagta ccctgatgag 3061 atcgagtaca tcttcaagcc atcctgtgtg cccctgatgc gatgcggggg ctgctgcaat 3121 gacgagggcc tggagtgtgt gcccactgag gagtccaaca tcaccatgca gattatgcgg 3181 atcaaacctc accaaggcca gcacatagga gagatgagct tcctacagca caacaaatgt 3241 gaatgcagac caaagaaaga tagagcaaga caagaaaaaa aatcagttcg aggaaaggga 3301 aaggggcaaa aacgaaagcg caagaaatcc cggtataagt cctggagcgt gtacgttggt 3361 gcccgctgct gtctaatgcc ctggagcctc cctggccccc atccctgtgg gccttgctca 3421 gagcggagaa agcatttgtt tgtacaagat ccgcagacgt gtaaatgttc ctgcaaaaac 3481 acagactcgc gttgcaaggc gaggcagctt gagttaaacg aacgtacttg cagatgtgac 3541 aagccgaggc ggggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 3601 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 3661 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3721 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3781 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3841 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 3901 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 3961 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4021 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 4081 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 4141 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 4201 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 4261 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 4321 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 4381 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 4441 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 4501 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 4561 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 4621 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 4681 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 4741 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 4801 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 4861 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 4921 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 4981 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 5041 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 5101 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5161 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 5221 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 5281 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 5341 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 5401 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 5461 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5521 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5581 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 5641 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5701 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5761 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5821 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5881 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 5941 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 6001 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6061 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6121 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 6181 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 6241 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 6301 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 6361 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 6421 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6481 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6541 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 6601 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 6661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 6721 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6781 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 6841 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 6901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7741 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 8881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 9061 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9121 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9181 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 9241 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 9301 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 9361 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9421 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9481 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.