pLV3-CMV-YTHDF3-FLAG-Puro plasmid

PVT109046

pLV3-CMV-YTHDF3-FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-YTHDF3(human)-FLAG-Puro
Alias: NM_152758.6;DF3
Gene lenght: 1755
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-YTHDF3-FLAG-Puro plasmid Description

pLV3-CMV-YTHDF3-FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-YTHDF3-FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgtcagcc actagcgtgg atcagagacc taaagggcaa 2341 ggaaataaag tttcagtaca aaacggttcg attcatcaaa aagatgctgt aaatgatgat 2401 gattttgagc catacttaag tagccagaca aatcagagta acagctatcc accaatgtca 2461 gatccataca tgcctagtta ctatgctcca tccattggat ttccatattc tcttggggaa 2521 gcagcgtggt ccacagctgg agaccagcct atgccatatc tgacaaccta tggacaaatg 2581 agtaatggag aacatcacta tataccagat ggtgtattta gtcaacctgg ggcattagga 2641 aatacccctc catttcttgg tcaacatgga tttaactttt ttcctggtaa tgctgatttc 2701 tctacatggg ggacaagtgg atctcaggga caatcaacac aaagttctgc ttatagtagc 2761 agttatggct atccacctag ttctcttggg agagctatta ctgatggaca ggctggattt 2821 ggcaatgata ctttgagtaa ggtgcctggc attagcagta ttgagcaagg catgactgga 2881 ctgaaaattg gtggtgacct gacagctgca gtgacaaaaa ctgtaggtac agctttgagc 2941 agcagtggta tgactagcat tgcaaccaat agtgtgcccc cagttagcag tgcagcacct 3001 aaaccaacct cctgggctgc cattgccaga aagcctgcca aacctcaacc gaaacttaaa 3061 cccaagggca atgtgggaat tgggggttct gctgtaccac cacctcctat aaaacacaac 3121 atgaatattg gaacttggga tgaaaaaggg tcagtggtaa aggctccacc aacccaacca 3181 gttctgcctc ctcaaactat aatccagcag cctcagccat taattcaacc accaccattg 3241 gtgcaaagcc aactgcctca acagcagcct caaccaccac aaccacagca gcaacaagga 3301 cctcagccac aggcccagcc tcaccaagtg cagcctcaac agcagcagct gcagaatcgc 3361 tgggtagctc ctcgtaacag gggagcaggc ttcaaccaga acaatggagc gggcagtgaa 3421 aactttggtt taggtgttgt acctgtcagt gcttcacctt ctagtgtaga agtgcatccc 3481 gtgctggaaa agctaaaggc cataaacaac tataatccca aagactttga ttggaatctg 3541 aagaatggac gtgtgtttat aattaaaagc tactctgagg atgacataca tcgttccatt 3601 aaatactcta tctggtgtag tactgagcat ggtaataagc gtttggatgc agcttaccgt 3661 tccctgaatg ggaaaggccc actctattta ctcttcagtg tgaatggcag tggacatttt 3721 tgtggagtgg ctgaaatgaa gtctgttgtg gactataatg cgtatgctgg tgtctggtct 3781 caggataagt ggaagggcaa atttgaagtt aaatggatct ttgtcaaaga tgttcccaat 3841 aaccaattac ggcatattcg cttagaaaat aatgacaaca aaccggttac caattcaagg 3901 gacactcaag aggtacccct agaaaaagct aagcaagtgc ttaaaataat tgctactttc 3961 aagcatacca cctcaatctt tgatgacttt gcacattatg aaaagcgtca agaagaggag 4021 gaagccatgc gtagggagag aaatagaaac aaacaaGGAT CCGATTACAA GGACGACGAT 4081 GACAAGTAGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC 4141 ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG 4201 AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC 4261 GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC 4321 GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC 4381 CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC 4441 TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG 4501 GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC 4561 CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA 4621 TCCAAGCTGT GACCGGCGCC TACGCTAGAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC 4681 CACCCGCGAC GACGTCCCCC GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC 4741 CGCCACGCGC CACACCGTCG ACCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA 4801 ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC 4861 CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT 4921 CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG 4981 CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC 5041 GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC 5101 CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA 5161 CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG 5221 GTGCATGACC CGCAAGCCCG GTGCCTGAGT CGACAATCAA CCTCTGGATT ACAAAATTTG 5281 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC 5341 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA 5401 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT 5461 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA 5521 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC 5581 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT 5641 GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG 5701 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG 5761 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT 5821 CTCCCTTTGG GCCGCCTCCC CGCCTGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG 5881 TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC 5941 GAAAATAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 6001 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 6061 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 6121 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT 6181 ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT 6241 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 6301 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC 6361 TATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 6421 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG 6481 AGGCTTTTTT GGAGGCCTAG ACTTTTGCAG AGACGGCCCA AATTCGTAAT CATGGTCATA 6541 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 6601 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 6661 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 6721 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 6781 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 6841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 6901 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 6961 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 7021 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 7081 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 7141 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 7201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 7261 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 7321 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 7381 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 7441 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 7501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 7561 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 7621 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 7681 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 7741 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 7801 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 7861 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 7921 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 7981 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 8041 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 8101 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 8161 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 8221 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 8281 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 8341 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 8401 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 8461 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 8521 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 8581 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 8641 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC 8701 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC 8761 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 8821 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 8881 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 8941 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC 9001 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG 9061 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC 9121 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.