pLV3-CMV-ZHX2-3×Myc-EF1a-CopGFP-Puro plasmid

PVT107452

pLV3-CMV-ZHX2-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ZHX2(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_001362797.2;AFR1; RAF
Gene lenght: 2511
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ZHX2-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-ZHX2-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-ZHX2-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg ctagcaaacg aaaatctaca actccatgca tggttcggac 2341 atcacaagta gtagaacaag atgtgcccga ggaagtagac agggccaaag agaaaggaat 2401 cggcacacca cagcctgacg tggccaagga cagttgggca gcagaacttg aaaactcttc 2461 caaagaaaac gaagtgatag aggtgaaatc tatgggggaa agccagtcca aaaaactcca 2521 aggtggttat gagtgcaaat actgccccta ctccacgcaa aacctgaacg agttcacgga 2581 gcatgtcgac atgcagcatc ccaacgtgat tctcaacccc ctctacgtgt gtgcagaatg 2641 taacttcaca accaaaaagt acgactccct atccgaccac aactccaagt tccatcccgg 2701 ggaggccaac ttcaagctga agttaattaa acgcaataat caaactgtct tggaacagtc 2761 catcgaaacc accaaccatg tcgtgtccat caccaccagt ggccctggaa ctggtgacag 2821 tgattctggg atctcggtga gtaaaacccc catcatgaag cctggaaaac caaaagcgga 2881 tgccaagaag gtgcccaaga agcccgagga gatcaccccc gagaaccacg tggaagggac 2941 cgcccgcctg gtgacagaca cagctgagat cctctcgaga ctcggcgggg tggagctcct 3001 ccaagacaca ttaggacacg tcatgccttc tgtacagctg ccaccaaata tcaaccttgt 3061 gcccaaggtc cctgtcccac taaatactac caaatacaac tctgccctgg atacaaatgc 3121 cacgatgatc aactctttca acaagtttcc ttacccgacc caggctgagt tgtcctggct 3181 gacagctgcc tccaaacacc cagaggagca catcagaatc tggtttgcca cccagcgctt 3241 aaagcatggc atcagctggt ccccagaaga ggtggaggag gcccggaaga agatgttcaa 3301 cggcaccatc cagtcagtac ccccgaccat cactgtgctg cccgcccagt tggcccccac 3361 aaaggtgacg cagcccatcc tccagacggc tctaccgtgc cagatcctcg gccagactag 3421 cctggtgctg actcaggtga ccagcgggtc aacaaccgtc tcttgctccc ccatcacact 3481 tgccgtggca ggagtcacca accatggcca gaagagaccc ttggtgactc cccaagctgc 3541 ccccgaaccc aagcgtccac acatcgctca ggtgccagag cccccaccca aggtggccaa 3601 ccccccgctc acaccagcca gtgaccgcaa gaagacaaag gagcagatag cacatctcaa 3661 ggccagcttt ctccagagcc agttccctga cgatgccgag gtttaccggc tcatcgaggt 3721 gactggcctt gccaggagcg agatcaagaa gtggttcagt gaccaccgat atcggtgtca 3781 aaggggcatc gtccacatca ccagcgaatc ccttgccaaa gaccagttgg ccatcgcggc 3841 ctcccgacac ggtcgcacgt atcatgcgta cccagacttt gccccccaga agttcaaaga 3901 gaaaacacag ggtcaggtta aaatcttgga agacagcttt ttgaaaagtt cttttcctac 3961 ccaagcagaa ctggatcggc taagggtgga gaccaagctg agcaggagag agatcgactc 4021 ctggttctcg gagaggcgga agcttcgaga cagcatggaa caagctgtct tggattccat 4081 ggggtctggc aaaaaaggcc aagatgtggg agcccccaat ggtgctctgt ctcgactcga 4141 ccagctctcc ggtgcccagt taacaagttc tctgcccagc ccttcgccag caattgcaaa 4201 aagtcaagaa caggttcatc tcctgaggag cacgtttgca agaacccagt ggcctactcc 4261 ccaggagtac gaccagttag cggccaagac tggcctggtc cgaactgaga ttgtgcgttg 4321 gttcaaggag aacagatgct tgctgaaaac gggaaccgtg aagtggatgg agcagtacca 4381 gcaccagccc atggcagatg atcacggcta cgatgccgta gcaaggaaag caacaaaacc 4441 catggccgag agcccaaaga acgggggtga tgtggttcca caatattaca aggaccccaa 4501 aaagctctgc gaagaggact tggagaagtt ggtgaccagg gtaaaagtag gcagcgagcc 4561 agcaaaagac tgtttgccag caaagccctc agaggccacc tcagaccggt cagagggcag 4621 cagccgggac ggccagggta gcgacgagaa cgaggagtcg agcgttgtgg attacgtgga 4681 ggtgacggtc ggggaggagg atgcgatctc agatagatca gatagctgga gtcaggctgc 4741 ggcagaaggt gtgtcggaac tggctgaatc agactccgac tgcgtccctg cagaggctgg 4801 ccaggccgga tccGAACAAA AACTAATATC AGAGGAAGAT TTGGAACAGA AACTAATCAG 4861 TGAAGAAGAT TTAGAGCAAA AGTTAATTTC CGAGGAGGAC TTATAgcggc cgcGaaggat 4921 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 4981 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 5041 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 5101 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 5161 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 5221 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 5281 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 5341 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 5401 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 5461 gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg 5521 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc 5581 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc 5641 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg 5701 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga 5761 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg 5821 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac 5881 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt 5941 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt 6001 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg 6061 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt 6121 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc 6181 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg 6241 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc 6301 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 6361 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 6421 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 6481 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 6541 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 6601 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 6661 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 6721 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 6781 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 6841 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta 6901 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 6961 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 7021 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 7081 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 7141 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 7201 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 7261 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 7321 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 7381 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac 7441 gagtcggatc tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7501 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7561 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7801 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7981 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 8041 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 8101 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca 8161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 8281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 8401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 8581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 8701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 8761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 8881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 8941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9001 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 9121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 9181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 9241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 9301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 9361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 9421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 9481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 9541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 9721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 9901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 9961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 10141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 10201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 10261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 10321 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 10381 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 10441 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 10501 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 10561 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 10621 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 10681 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 10741 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.