pLV3-CMV-ZIC3-3×FLAG-CopGFP-Puro plasmid

PVT105255

pLV3-CMV-ZIC3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ZIC3(cattle)-3×FLAG-CopGFP-Puro
Alias: NM_001192813.1
Gene lenght: 1395
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Bovine
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ZIC3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ZIC3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-ZIC3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga cgatgctcct ggacggaggc 2341 ccgcagttcc caggactggg agtgggcagc ttcggcgcgc cgcgccacca cgagatgccc 2401 aaccgcgagc cggcgggcat agggctgact cctttcgggg actcgcccca cgccgccgcc 2461 gccgccgccg ccgccttcaa gctgagcccc gcggcggctc acgatctgtc ttctggccag 2521 agctcggcgt tcacgccgca gggttcgggt tacgccaacg ccctgggcca tcatcaccac 2581 caccatcacc accatcacca cgccggccag gtgcccagct acggcggagc cgcctccgcc 2641 gccttcaact ccacgcgcga ctttctgttc cgccagcgcg gctccgggct cggcgaggcg 2701 gcctcgggtg gcgggcagca cgggctcttc gccggctcgg cgagtagcct gcacgctccg 2761 gctggcatct ctgagccccc tggctacctg ctcttccccg ggctgcacga ccaaggcgct 2821 gggcacccgt cgcccaccgg gcacgtggac aacaaccagg tccacctggg gctgcgcggg 2881 gagctgttcg gccgcgctga cccgtaccgc ccggtggcca gcccgcgcac ggacccctac 2941 acggccggcg cgcagttccc gaactacagc cccatgaaca tgaacatggg cgtgaacgtg 3001 gcggcccacc acgggcccgg cgccttcttc cgttacatgc gacagcccat caagcaggag 3061 ctgtcgtgca agtggatcga cgaggctcag ctgagccggc ccaagaagag ctgtgaccgg 3121 accttcagca ccatgcacga gctggtgaca catgtcacca tggagcatgt ggggggcccg 3181 gagcagaaca accacgtctg ctattgggag gagtgccccc gcgagggcaa gtctttcaag 3241 gcgaagtaca aactggtcaa tcacattcgg gtgcacacgg gcgagaagcc cttcccgtgc 3301 cccttcccgg gctgcgggaa gatcttcgct cgctccgaga acctcaagat ccacaagagg 3361 acccacacag gtgagaaacc ttttaaatgt gaatttgaag gctgtgacag acgctttgcc 3421 aacagcagcg accgcaagaa gcacatgcat gtgcacacct cggacaagcc ctatatctgc 3481 aaagtgtgcg acaagtccta cacgcacccg agctccctgc gcaagcacat gaaggttcat 3541 gaatctcaag ggtcagattc ctcccctgct gccagttcag gctatgaatc ttccactcca 3601 cccgctatag cttctgcaaa cagtaaagat accactaaaa ccccttctgc agttcaaact 3661 agcaccagcc acaaccctgg acttcctccc aattttaacg aatggtacgt cggatccGAC 3721 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 3781 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 3841 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 3901 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 3961 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 4021 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 4081 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 4141 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 4201 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 4261 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 4321 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 4381 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 4441 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 4501 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 4561 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 4621 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 4681 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 4741 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 4801 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 4861 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 4921 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 4981 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 5041 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 5101 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 5161 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5221 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 5281 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 5341 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 5401 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5461 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5521 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5581 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5641 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5701 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5761 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5821 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5881 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5941 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 6001 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 6061 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 6121 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 6181 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 6241 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 6301 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 6361 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 6421 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 6481 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 6541 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6601 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6661 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6721 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6841 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 6901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 6961 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 7021 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 7081 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 7141 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 7201 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 7261 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 7321 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 7381 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7441 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7501 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7561 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7621 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7681 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7741 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7801 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7861 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7921 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7981 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 8041 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 8101 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 8161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 8221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 8281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 8341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 8401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 8581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 9001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 9061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 9121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 9181 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 9241 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 9301 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 9361 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 9421 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 9481 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 9541 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 9601 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 9661 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???