pLV3-CMV-ZNF185-3×FLAG-CopGFP-Puro plasmid

PVT91976

pLV3-CMV-ZNF185-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ZNF185(human)-3×FLAG-CopGFP-Puro
Alias: NM_001178106.1;SCELL
Gene lenght: 2163bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ZNF185-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ZNF185-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ZNF185-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga gtatctcagc tcttggaggc 2341 cgcaccaaag ggaagcctct gccaccaggc gaggaggagc gcaataacgt tctcaagcag 2401 atgaaagtgc gaaccacgct gaagggggac aagagctgga ttaccaagca ggatgaatcg 2461 gagggtcgca ccatagagct gccctcaggc cggagtcgcg ccacatcctt ttcatcagct 2521 ggggaggttc cgaagcctag gcctccgagc acaagggctc ccactggcta catcatccgg 2581 ggagtgttca ccaagcccat agacagctct tcccagcccc agcagcagtt ccccaaggcc 2641 aacgggactc caaaaagtgc tgccagtctg gtgagaacag ctaacgctgg tcctccccgc 2701 ccctcctcct ctggctacaa gatgaccact gaggattaca agaagctggc accctacaat 2761 atcaggcgca gctctacatc aggggacacc gaggaggagg aggaggagga ggtggtgcca 2821 ttctcctcag atgaacagaa acggaggtca gaggctgcaa gcggtgttct gaggaggaca 2881 gctccccggg agcactccta cgtcctgtca gcggccaaga agagcactgg tcctacccag 2941 gagacacagg caccgtttat cgcgaagagg gtggaggtgg tggaagagga cgggccttct 3001 gagaagagcc aggacccacc tgctctggca agatccactc ctggctcaaa cagtgcggat 3061 ggaggcagga ccaaagcgtc tcgggcaatt tggatcgagt gcctgccaag tatgcctagc 3121 cccgctggga gccaggagct cagctcaaga ggtgaggaaa ttgtccgcct gcagatcctg 3181 acacccaggg caggactccg cctggtggcc ccagacgtgg aaggcatgag gtcttcccca 3241 ggcaacaaag acaaggaggc cccctgctcc agagagctcc agagggactt ggctggtgag 3301 gaggctttca gggcccccaa cacagatgct gcaaggtcaa gtgcacagtt gagtgatggc 3361 aatgtgggat ccggagccac gggctcccgg cctgaaggct tggctgcagt agacatcggc 3421 tccgagagag gaaggctgtg tgcagctgct agctttgcct ctttcctgga agaccaggat 3481 gggcacagtg ccaactctca gtcctgcaag cctagaccag cagccatcag ctccagtgcc 3541 acttcagtct ctgctgtccc tgctgatagg aagagcaaca gcacagcagc ccaggaggat 3601 gcaaaggcag acccaaaggg ggccttggct gattatgagg ggaaggatgt ggccaccagg 3661 gtcggagagg cctggcagga gaggcctgga gctccaagag gtggccaagg agacccagct 3721 gtacccgctc agcaacctgc agatcccagc accccagagc ggcagagcag ccccagcgga 3781 tctgagcaac ttgtcagacg agagagttgt ggcagcagcg tgttgactga ttttgagggg 3841 aaggatgtgg ccaccaaggt cggagaggcc tggcaggaca ggcctggagc cccaagaggt 3901 ggccaaggag acccagctgt acccactcag caacctgcag atcccagtac cccagaacag 3961 cagaacagcc ccagcggatc tgagcaattc gtcagacgag agagctgcac cagcagggtg 4021 aggagcccct cgagctgcat ggtcactgtt actgtcactg ccacatctga gcagcctcac 4081 atttatattc cagcccccgc aagtgaattg gactccagct ctaccaccaa agggattctc 4141 ttcgtgaagg agtacgtgaa tgctagtgaa gtgtcttctg ggaagccagt atctgcacgc 4201 tatagcaacg tcagcagcat tgaggactca ttcgccatgg agaagaagcc tccatgtggc 4261 agcactccat actctgagag gacaactgga gggatctgta cttactgcaa ccgtgagatc 4321 cgagactgtc caaagattac cctagaacat cttggtatct gctgccatga atattgcttt 4381 aagtgtggga tttgcagtaa accgatgggc gatctcctgg atcagatctt cattcaccgt 4441 gacaccattc actgtgggaa atgctatgag aagctcttcg gatccGACTA CAAAGACCAT 4501 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 4561 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4621 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4681 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4741 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4801 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4861 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4921 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4981 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 5041 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 5101 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 5161 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 5221 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 5281 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 5341 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 5401 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 5461 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 5521 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 5581 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 5641 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 5701 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 5761 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 5821 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 5881 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 5941 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 6001 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 6061 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 6121 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 6181 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 6241 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 6301 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6361 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6421 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6481 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 6541 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6601 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6661 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6721 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6781 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6841 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6901 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 6961 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 7021 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 7081 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 7141 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 7201 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 7261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 7321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 7441 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7621 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 7681 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7741 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 7801 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 7861 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7921 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7981 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8041 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8101 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8641 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 9001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 9061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 9121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 9181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 9241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9961 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 10021 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 10081 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 10141 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 10201 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 10261 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 10321 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10381 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10441 cagtgccaag ctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???