pLV3-EF1a-3×HA-TRMT61A-T2A-Blast plasmid

PVT90431

pLV3-EF1a-3×HA-TRMT61A-T2A-Blast plasmid Information

Plasmid name: pLV3-EF1a-3×HA-TRMT61A(human)-T2A-Blast
Alias: NM_152307.3;C14orf172; GCD14; Gcd14p; hTRM61; TRM61
Gene lenght: 867bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-3×HA-TRMT61A-T2A-Blast plasmid Description

pLV3-EF1a-3×HA-TRMT61A-T2A-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-3×HA-TRMT61A-T2A-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcGCCAC CATGTACCCA TACGACGTAC CTGATTATGC 2521 AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC 2581 Aatgagcttc gtggcatacg aggagctgat caaggagggt gacacggcca tcctgtcact 2641 gggccatggt gcaatggtgg cggtgcgtgt gcagcgtggg gcacagaccc agacccggca 2701 tggtgtcctg cggcactcag ttgaccttat cggccgcccc ttcggctcca aggtgacgtg 2761 cggccgaggt ggctgggtgt atgtgctgca ccccacgccc gagctctgga cgctgaacct 2821 gccgcaccgc acgcagatcc tctactccac agacatcgcc ctcatcacca tgatgttgga 2881 gcttcggccc ggctctgtgg tctgtgagtc tggcaccggc agtggctctg tgtcccacgc 2941 catcatccgc accattgcac ccacgggtca cctgcacacg gtggagttcc accagcagcg 3001 ggcagagaag gcccgggagg agttccagga gcaccgtgtg ggccgctggg tgactgtgcg 3061 cacccaggac gtgtgccgca gtggctttgg cgtgagccac gtggccgacg ccgtcttcct 3121 ggacatccca tcaccctggg aggccgtggg ccacgcctgg gacgccctca aggtcgaagg 3181 cgggcgcttc tgctccttct caccgtgcat cgagcaggtg caacgcacat gccaggcgct 3241 ggcagcgcgc ggcttctcag agctgagcac cctggaggtg ctgccacagg tctacaacgt 3301 gcgcactgtc agcctgccac cgcccgacct gggcacaggc acagatggcc ctgccggctc 3361 cgacaccagc cccttccgca gcggcacgcc catgaaggag gccgtgggcc acaccggcta 3421 cctgaccttc gccaccaaga ccccaggcgg atccgcggcc gctgagggca gaggaagtct 3481 tctaacatgc ggtgacgtgg aggagaatcc cggcccttcc gggATGGCCA AGCCTTTGTC 3541 TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA ATCAACAGCA TCCCCATCTC 3601 TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC GGCCGCATCT TCACTGGTGT 3661 CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC GTGGTGCTGG GCACTGCTGC 3721 TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC GGAAATGAGA ACAGGGGCAT 3781 CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT CTGCATCCTG GGATCAAAGC 3841 CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC 3901 TGGTTATGTG TGGGAGGGCT AAgtcgacaa tcaacctctg gattacaaaa tttgtgaaag 3961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 4081 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 4141 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 4201 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 4261 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 4321 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 4381 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 4441 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 4501 ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 4561 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat 4621 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 4681 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 4741 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 4801 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 4861 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 4921 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 4981 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 5041 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 5101 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 5161 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt 5221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 5281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 5341 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 5401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 5461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 5521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 5581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 5701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 5821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 6061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 6121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 6181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 6241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 6301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 6361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 6721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6961 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 7021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 7141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 7321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 7381 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 7441 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 7501 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 7561 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 7621 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 7681 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 7741 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 7801 acgacgttgt aaaacgacgg ccagtgccaa gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.