pLV3-EF1a-Fbp1-3×HA-PGK-CopGFP-Puro plasmid

PVT107289

pLV3-EF1a-Fbp1-3×HA-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-Fbp1(mouse)-3×HA-PGK-CopGFP-Puro
Alias: NM_019395.3;Fbp-2; Fbp2; Fbp3
Gene lenght: 1014
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-Fbp1-3×HA-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-Fbp1-3×HA-PGK-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-EF1a-Fbp1-3×HA-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cGCCACCatg gcgaaccatg cgcccttcga 2521 aacggatatc agcaccctga cccgcttcgt catggagcag ggcaggaagg ctcagggcac 2581 gggggagttg acccagctgc tgaattcgct ctgcaccgcg atcaaagcca tctcgtctgc 2641 ggtgcgccag gcgggcatcg cacagctcta tggtatcgct ggctcaacca atgtgactgg 2701 ggatcaagta aagaagctgg acatactttc caatgacctg gtgatcaata tgctgaagtc 2761 gtcctacgct acctgtgttc ttgtgtctga agaaaacaca aatgccatca taatcgaacc 2821 tgagaagagg ggcaaatatg ttgtctgttt cgatcccctt gatggctcat ccaacattga 2881 ctgccttgtg tccatcggaa ccatttttgg catttacaga aagaaaagta ctgatgagcc 2941 ttctgagaag gatgctctgc agcccggccg ggacctggtg gcagccgggt atgcgctcta 3001 tggcagtgcc accatgttgg tccttgccat ggattgtggt gtcaactgct tcatgctgga 3061 cccgtccatt ggagaattca ttatggtgga cagggacgtg aagatgaaga agaaaggtaa 3121 catctacagc cttaatgagg gttatgccaa ggactttgac cctgccatca atgagtatct 3181 ccagaggaaa aagttccctc cggatggttc agccccctat ggtgcccggt atgtggggtc 3241 catggtggct gatattcacc gcactctggt atatggaggg atctttttat accccgccaa 3301 caagaaaagc ccaagtggaa agctgcggct gctgtatgag tgcaacccca tagcttatgt 3361 catggagaag gccggtgggc tcgccaccac gggggacaaa gatatattag acatcgttcc 3421 caccgagatc caccagaagg caccagtcgt catggggtcc tctgaagatg tgcaggagtt 3481 cctggagatc tacaggaagc acaaagccaa gTACCCATAC GACGTACCTG ATTATGCAAC 3541 TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT GATGTCCCAG ACTACGCATA 3601 Gggatccgcg gccgctgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct 3661 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc 3721 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct 3781 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga 3841 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg 3901 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct 3961 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg 4021 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc 4081 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatgg 4141 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga 4201 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga 4261 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg 4321 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc 4381 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg 4441 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca 4501 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca 4561 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg 4621 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc 4681 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc 4741 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct 4801 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg 4861 gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg 4921 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca 4981 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 5041 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 5101 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 5161 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 5221 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 5281 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 5341 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 5401 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 5461 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 5521 gcatgacccg caagcccggt gcctgagtcg acaatcaacc tctggattac aaaatttgtg 5581 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 5641 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 5701 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 5761 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 5821 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 5881 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 5941 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 6001 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 6061 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 6121 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta 6181 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 6241 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 6301 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 6361 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 6421 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 6481 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 6541 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 6601 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 6661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 6721 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 6781 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc 6841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6901 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6961 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 7021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 7081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 7141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 7201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 7681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 8041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 8101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 8161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 8221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 9001 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 9061 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 9121 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 9181 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 9241 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 9301 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 9361 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 9421 agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.