pLV3-EF1a-GAL4BD-Elk1-PGK-Klb-IRES-Puro plasmid

PVT92855

pLV3-EF1a-GAL4BD-Elk1-PGK-Klb-IRES-Puro plasmid Information

Plasmid name: pLV3-EF1a-GAL4BD-Elk1(mouse)-PGK-Klb(mouse)-IRES-Puro
Alias: NM_007922.5;Elk-1/NM_031180.3;betaKlotho
Gene lenght: 1290/3132bpbp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEF-F
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-GAL4BD-Elk1-PGK-Klb-IRES-Puro plasmid Description

pLV3-EF1a-GAL4BD-Elk1-PGK-Klb-IRES-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-GAL4BD-Elk1-PGK-Klb-IRES-Puro plasmid Sequence

1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttacgc 3361 gtgccaccat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa 3421 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt 3481 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg 3541 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg 3601 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg 3661 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata 3721 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta 3781 acaaaggtca aagacagttg actgtatcgg gtggaggcgg atcaggaggc ggtggatctg 3841 ctagcatgga cccatctgtg acgctgtggc agtttctgct gcagcttctg agagaacaag 3901 gtaatggcca catcatctcc tggacctcac gggatggtgg tgagttcaag ttggtggatg 3961 cagaggaggt ggcccggcta tggggactgc gcaagaacaa gaccaacatg aattacgaca 4021 agcttagccg ggccttgcgg tactactatg ataagaatat catccgcaag gtgagcggcc 4081 agaagtttgt ctacaagttt gtgtcctacc cagaggttgc agggtgctcc actgaagact 4141 gcccacccca gcctgaggtg tctgtaacct cggccatagc catggcccct gctactgtcc 4201 atgcaggccc aggggacact gccactggaa agccaggaac accaaagggt gcaggaatga 4261 caggccaagg tggcttagca cgaagcagcc ggaatgaata catgcgctcg ggcctctatt 4321 ctaccttcac aatacaatcc ctgcagccac agccacagcc acccattcct cctcggcctg 4381 cctcagtgct tcccaacact acccctgcag gagtaccagc acccgcctca gggagcagga 4441 gcaccagtcc aaacccctta gaagcctgtt tggaagcaga agaggctggt ctgcccctgc 4501 aggttatcct aaccccacca gaggccccaa accagaaatc cgaagagttg agtctggacc 4561 caagttttgg ccatccacag cccccagaag tcaaagtgga ggggcctaag gaagaattgg 4621 aagctgcaag ggctggaggc ttcagttcag aagctgtcaa agctgaacca gaagtctcag 4681 cctcagaagg cctgctggct cggctcccag ccatcctaac agagaacaca gcccaggtgt 4741 gtggcctctc cacttccacc actgagatca cccaaccgca gaagggccga aagcctcggg 4801 acctggaact tccacttagc ccaagcctgc tgggtggcca gggacctgaa cggactccag 4861 gatcaggaac aagctctggt cttcaggcac cggggccagc gctaacgcca tccctgctcc 4921 ccacacatac cttgaccccg gtgctgctga cacccagctc gctgccccct agcatccatt 4981 tctggagcac tctgagtcca attgcacccc gtagtccagc caagctctcc ttccagtttc 5041 cgtccagtgg cagcgcacag gtgcacatcc cttccatcag tgtggatggc ctctcgaccc 5101 ccgtggtgct ctccccaggg ccccagaagc catgaGGGTA GGGGAGGCGC TTTTCCCAAG 5161 GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT 5221 CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG 5281 CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT 5341 CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA 5401 CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG 5461 CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG 5521 CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT 5581 TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGGCCAC 5641 Catgaagaca ggctgtgcag cagggtctcc ggggaatgaa tggattttct tcagctctga 5701 tgaaagaaac acacgctcta ggaaaacaat gtccaacagg gcactgcaaa gatctgccgt 5761 gctgtctgcg tttgttctgc tgcgagctgt taccggcttc tccggagacg ggaaagcaat 5821 atgggataaa aaacagtacg tgagtccggt aaacccaagt cagctgttcc tctatgacac 5881 tttccctaaa aacttttcct ggggcgttgg gaccggagca tttcaagtgg aagggagttg 5941 gaagacagat ggaagaggac cctcgatctg ggatcggtac gtctactcac acctgagagg 6001 tgtcaacggc acagacagat ccactgacag ttacatcttt ctggaaaaag acttgttggc 6061 tctggatttt ttaggagttt ctttttatca gttctcaatc tcctggccac ggttgtttcc 6121 caatggaaca gtagcagcag tgaatgcgca aggtctccgg tactaccgtg cacttctgga 6181 ctcgctggta cttaggaata tcgagcccat tgttaccttg taccattggg atttgcctct 6241 gacgctccag gaagaatatg ggggctggaa aaatgcaact atgatagatc tcttcaacga 6301 ctatgccaca tactgcttcc agacctttgg agaccgtgtc aaatattgga ttacaattca 6361 caacccttac cttgttgctt ggcatgggtt tggcacaggt atgcatgcac caggagagaa 6421 gggaaattta acagctgtct acactgtggg acacaacctg atcaaggcac attcgaaagt 6481 gtggcataac tacgacaaaa acttccgccc tcatcagaag ggttggctct ccatcacctt 6541 ggggtcccat tggatagagc caaacagaac agacaacatg gaggacgtga tcaactgcca 6601 gcactccatg tcctctgtgc ttggatggtt cgccaacccc atccacgggg acggcgacta 6661 ccctgagttc atgaagacgg gcgccatgat ccccgagttc tctgaggcag agaaggagga 6721 ggtgaggggc acggctgatt tctttgcctt ttccttcggg cccaacaact tcaggccctc 6781 aaacaccgtg gtgaaaatgg gacaaaatgt atcactcaac ttaaggcagg tgctgaactg 6841 gattaaactg gaatacgatg accctcaaat cttgatttcg gagaacggct ggttcacaga 6901 tagctatata aagacagagg acaccacggc catctacatg atgaagaatt tcctaaacca 6961 ggttcttcaa gcaataaaat ttgatgaaat ccgcgtgttt ggttatacgg cctggactct 7021 cctggatggc tttgagtggc aggatgccta tacgacccga cgagggctgt tttatgtgga 7081 ctttaacagt gagcagaaag agaggaaacc caagtcctcg gctcattact acaagcagat 7141 catacaagac aacggcttcc ctttgaaaga gtccacgcca gacatgaagg gtcggttccc 7201 ctgtgatttc tcttggggag tcactgagtc tgttcttaag cccgagttta cggtctcctc 7261 cccgcagttt accgatcctc acctgtatgt gtggaatgtc actggcaaca gattgctcta 7321 ccgagtggaa ggggtaaggc tgaaaacaag accatcccag tgcacagatt atgtgagcat 7381 caaaaaacga gttgaaatgt tggcaaaaat gaaagtcacc cactaccagt ttgctctgga 7441 ctggacctct atccttccca ctggcaatct gtccaaagtt aacagacaag tgttaaggta 7501 ctataggtgt gtggtgagcg aaggactgaa gctgggcgtc ttccccatgg tgacgttgta 7561 ccacccaacc cactcccatc tcggcctccc cctgccactt ctgagcagtg gggggtggct 7621 aaacatgaac acagccaagg ccttccagga ctacgctgag ctgtgcttcc gggagttggg 7681 ggacttggtg aagctctgga tcaccatcaa tgagcctaac aggctgagtg acatgtacaa 7741 ccgcacgagt aatgacacct accgtgcagc ccacaacctg atgatcgccc atgcccaggt 7801 ctggcacctc tatgataggc agtataggcc ggtccagcat ggggctgtgt cgctgtcctt 7861 acattgcgac tgggcagaac ctgccaaccc ctttgtggat tcacactgga aggcagccga 7921 gcgcttcctc cagtttgaga tcgcctggtt tgcagatccg ctcttcaaga ctggcgacta 7981 tccatcggtt atgaaggaat acatcgcctc caagaaccag cgagggctgt ctagctcagt 8041 cctgccgcgc ttcaccgcga aggagagcag gctggtgaag ggtaccgtcg acttctacgc 8101 actgaaccac ttcactacga ggttcgtgat acacaagcag ctgaacacca accgctcagt 8161 tgcagacagg gacgtccagt tcctgcagga catcacccgc ctaagctcgc ccagccgcct 8221 ggctgtaaca ccctggggag tgcgcaagct ccttgcgtgg atccggagga actacagaga 8281 cagggatatc tacatcacag ccaatggcat cgatgacctg gctctagagg atgatcagat 8341 ccgaaagtac tacttggaga agtatgtcca ggaggctctg aaagcatatc tcattgacaa 8401 ggtcaaaatc aaaggctact atgcattcaa actgactgaa gagaaatcta agcctagatt 8461 tggatttttc acctctgact tcagagctaa gtcctctgtc cagttttaca gcaagctgat 8521 cagcagcagt ggcctccccg ctgagaacag aagtcctgcg tgtggtcagc ctgcggaaga 8581 cacagactgc accatttgct catttctcgt ggagaagaaa ccactcatct tcttcggttg 8641 ctgcttcatc tccactctgg ctgtactgct atccatcacc gtttttcatc atcaaaagag 8701 aagaaaattc cagaaagcaa ggaacttaca aaatatacca ttgaagaaag gccacagcag 8761 agttttcagc taaactagtt ctagagcggc cgcggatccc gcccctctcc ctcccccccc 8821 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 8881 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 8941 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 9001 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 9061 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 9121 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 9181 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 9241 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt 9301 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 9361 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca 9421 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg 9481 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc 9541 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 9601 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 9661 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 9721 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 9781 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 9841 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 9901 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 9961 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaggtc gacaatcaac 10021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 10081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 10141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 10201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 10261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 10321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 10381 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 10441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 10501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 10561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt 10621 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 10681 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 10741 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 10801 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 10861 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 10921 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 10981 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 11041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 11101 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 11161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 11221 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 11281 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 11341 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 11401 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 11461 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 11521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 11581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 11641 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 11701 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 11761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 11821 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 11881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 11941 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 12001 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 12061 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 12121 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 12181 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 12241 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 12301 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 12361 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 12421 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 12481 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 12541 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 12601 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 12661 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 12721 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 12781 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 12841 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 12901 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 12961 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 13021 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 13081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 13141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 13201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 13261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 13321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 13381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 13441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 13501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 13561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 13621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 13681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 13741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 13801 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 13861 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 13921 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 13981 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 14041 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 14101 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 14161 gc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???