pLV3-EF1a-H2AZ2-HA-Puro plasmid

PVT102679

pLV3-EF1a-H2AZ2-HA-Puro plasmid Information

Plasmid name: pLV3-EF1a-H2AZ2(human)-HA-Puro
Alias: NM_012412.5;H2A.Z-2; H2AFV; H2AV
Gene lenght: 384
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-H2AZ2-HA-Puro plasmid Description

pLV3-EF1a-H2AZ2-HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-H2AZ2-HA-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gctggaggca aagctggaaa 2521 ggacagtggg aaggccaagg ctaaggcagt atctcgctca cagagagctg ggctacagtt 2581 tcctgtgggc cgcatccaca gacacttgaa gactcgcacc acaagccatg gaagggtggg 2641 tgccactgct gccgtgtaca gtgctgcgat tctggagtac ctcactgcag aggtgctgga 2701 gctggcaggt aatgcttcta aggatctcaa agtaaagcgt atcactccgc gtcacttgca 2761 gcttgcaatc cgtggtgatg aagagttgga ttctcttatc aaggctacca tagctggggg 2821 tggtgtgatc cctcacatcc acaaatctct gattggaaag aagggacagc agaaaactgc 2881 tggatccTAC CCATACGATG TTCCAGATTA CGCTTAGgcg gccgctgggt aggggaggcg 2941 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 3001 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 3061 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 3121 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 3181 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 3241 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 3301 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 3361 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 3421 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca 3481 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 3541 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 3601 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 3661 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 3721 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 3781 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 3841 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 3901 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 3961 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 4021 gcatgacccg caagcccggt gcctgagtcg acaatcaacc tctggattac aaaatttgtg 4081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 4141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 4201 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 4261 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 4321 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 4381 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 4441 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 4501 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 4561 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 4621 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta 4681 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 4741 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 4801 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 4861 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 4921 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 4981 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5041 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5101 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 5161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 5281 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc 5341 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5401 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5461 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5521 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 5581 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5641 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5701 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5761 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5821 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5881 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5941 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6001 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6061 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6121 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 6181 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6241 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6301 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6361 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6421 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 6481 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6541 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 6601 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 6661 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 6721 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 6781 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 6841 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 6901 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 6961 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7021 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7081 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7141 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7201 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7261 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7321 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7381 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7441 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 7501 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 7561 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 7621 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 7681 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 7741 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 7801 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 7861 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 7921 agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???