pLV3-EF1a-MCS-TurboID-FLAG-PGK-CopGFP-Puro plasmid

PVT108874

pLV3-EF1a-MCS-TurboID-FLAG-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-MCS-TurboID-FLAG-PGK-CopGFP-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-MCS-TurboID-FLAG-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-MCS-TurboID-FLAG-PGK-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-MCS-TurboID-FLAG-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgGATATCCA GCTCGAGATG ggtggaggcg 2521 gatcaAAAGA CAATACTGTG CCTCTGAAGC TGATCGCTCT CCTGGCTAAT GGCGAGTTCC 2581 ATAGTGGCGA ACAGCTGGGA GAAACCCTGG GCATGTCCAG GGCCGCTATC AACAAGCACA 2641 TTCAGACTCT GCGCGACTGG GGCGTGGACG TGTTCACCGT GCCCGGAAAG GGCTACTCTC 2701 TGCCCGAGCC TATCCCGCTG CTGAACGCTA AACAGATTCT GGGACAGCTG GACGGCGGGA 2761 GCGTGGCAGT CCTGCCTGTG GTCGACTCCA CCAATCAGTA CCTGCTGGAT CGAATCGGCG 2821 AGCTGAAGAG TGGGGATGCT TGCATTGCAG AATATCAGCA GGCAGGGAGA GGAAGCAGAG 2881 GGAGGAAATG GTTCTCTCCT TTTGGAGCTA ACCTGTACCT GAGTATGTTT TGGCGCCTGA 2941 AGCGGGGACC AGCAGCAATC GGCCTGGGCC CGGTCATCGG AATTGTCATG GCAGAAGCGC 3001 TGCGAAAGCT GGGAGCAGAC AAGGTGCGAG TCAAATGGCC CAATGACCTG TATCTGCAGG 3061 ATAGAAAGCT GGCAGGCATC CTGGTGGAGC TGGCCGGAAT AACAGGCGAT GCTGCACAGA 3121 TCGTCATTGG CGCCGGGATT AACGTGGCTA TGAGGCGCGT GGAGGAAAGC GTGGTCAATC 3181 AGGGCTGGAT CACACTGCAG GAAGCAGGGA TTAACCTGGA CAGGAATACT CTGGCCGCTA 3241 CGCTGATCCG AGAGCTGCGG GCAGCCCTGG AACTGTTCGA GCAGGAAGGC CTGGCTCCAT 3301 ATCTGCCACG GTGGGAGAAG CTGGATAACT TCATCAATAG ACCCGTGAAG CTGATCATTG 3361 GGGACAAAGA GATTTTCGGG ATTAGCCGGG GGATTGATAA ACAGGGAGCC CTGCTGCTGG 3421 AACAGGACGG AGTTATCAAA CCCTGGATGG GCGGAGAAAT CAGTCTGCGG TCTGCCGAAA 3481 AGggaggcgg tggatctGAC TACAAGGACG ACGATGACAA Gtaggatccg cggccgctgg 3541 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 3601 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 3661 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 3721 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 3781 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 3841 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 3901 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 3961 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 4021 tcatctccgg gcctttcgac ctgcagccca agcttaccat ggagagcgac gagagcggcc 4081 tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc 4141 tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca 4201 ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct 4261 accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg 4321 gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct 4381 tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct 4441 tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc 4501 acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc 4561 gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc 4621 accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc 4681 acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct 4741 tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct 4801 ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc 4861 ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 4921 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 4981 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 5041 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 5101 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 5161 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 5221 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 5281 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 5341 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 5401 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 5461 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5521 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5581 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 5641 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 5701 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 5761 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 5821 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 5881 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 5941 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6001 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 6061 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6121 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt 6181 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 6241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 6301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 6361 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 6421 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 6481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 6541 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 6601 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 6661 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 6721 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt 6781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7561 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 7621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 7981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8881 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 8941 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 9001 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 9061 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 9121 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 9181 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 9241 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 9301 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 9361 cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.