pLV3-EF1a-PRDM15-HA-Puro plasmid

PVT105084

pLV3-EF1a-PRDM15-HA-Puro plasmid Information

Plasmid name: pLV3-EF1a-PRDM15(human)-HA-Puro
Alias: NM_001040424.3;C21orf83; PFM15; ZNF298
Gene lenght: 3423
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF-1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: 5' LTR (truncated) -F1
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-PRDM15-HA-Puro plasmid Description

pLV3-EF1a-PRDM15-HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-PRDM15-HA-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gctgaagatg ggagcgaaga 2521 gatcatgttc atctggtgtg aagactgcag ccagtaccac gactccgaat gtcccgagct 2581 gggcccagtg gtcatggtca aagactcctt tgtgttaagc agggcaaggt catcccttcc 2641 tcccaacttg gagatcagac gactggaaga tggagccgag ggggtgttcg ccatcactca 2701 gctcgtcaag cggacacagt tcggtccctt tgagtccagg agggtcgcca aatgggaaaa 2761 ggagtctgca tttcccctga aggtgttcca gaaggacggg caccccgtgt gcttcgacac 2821 ctccaacgag gatgactgca actggatgat gctggtgcgg ccagcggcgg aggccgagca 2881 ccagaacctg acggcctacc agcacggcag cgacgtgtac ttcaccacct ccagagacat 2941 ccccccgggt accgagctgc gcgtgtggta tgcggccttc tatgccaaga agatggacaa 3001 gcccatgctg aagcaggccg gctctggcgt ccacgctgca ggcaccccag aaaacagcgc 3061 ccccgtggag tcggagccca gccagtgggc gtgtaaagtg tgttctgcca ccttcctgga 3121 gctgcagctc ctcaatgaac atctgttggg ccacttagaa caagccaaaa gccttcctcc 3181 aggcagccaa agcgaggcag cagctcccga gaaggagcag gacacacccc ggggggaacc 3241 ccctgcagtg cccgagagcg agaatgttgc caccaaagaa cagaagaaaa agcctcgaag 3301 ggggagaaaa cccaaagtgt ccaaagctga gcagcctcta gtcatcgtgg aagacaagga 3361 acccacagag caagtggcag agatcattac cgaggtccct ccggatgagc ctgtgagtgc 3421 aacgccagat gagcggatca tggagctggt tctggggaag ctggccacca ccaccactga 3481 caccagctcg gttccaaagt tcacccatca tcagaataac accatcacgc tcaagaggag 3541 cttaattctc tcaagcagac acggcatccg gcgcaagctc atcaaacagc tcggggagca 3601 caagcgggtt taccagtgca atatctgcag caagatcttc cagaacagca gcaacctgag 3661 caggcacgtg cgctcgcatg gtgacaagct gtttaagtgc gaagagtgtg caaaattgtt 3721 cagccgcaaa gagagcctaa agcagcacgt ttcctacaag cacagcagga acgaggtgga 3781 cggcgagtac aggtaccgct gcggcacttg tgagaagacc ttccgcatcg agagcgcgct 3841 ggagttccac aactgcagga cagatgacaa gacgttccaa tgtgagatgt gtttcagatt 3901 cttctccacc aacagcaacc tctccaagca caagaagaag cacggcgaca agaagtttgc 3961 ctgtgaggtc tgcagcaaga tgttctaccg caaggacgtc atgctggacc accagcgccg 4021 gcacctggaa ggagtgcggc gagtgaagcg agaggacctg gaggccggtg gggagaacct 4081 ggtccgttac aagaaggagc cttccgggtg cccggtgtgt ggcaaggtgt tctcctgccg 4141 gagcaatatg aacaagcacc tgctcaccca cggcgacaag aagtacacct gcgagatctg 4201 cgggcgcaag ttcttccgcg tggatgtgct cagggaccac atccatgtcc acttcaagga 4261 catcgcgttg atggatgacc accagaggga agagtttatc ggcaagatcg ggatctcctc 4321 ggaagaaaac gatgacaatt ctgacgagag cgcagactcg gagcctcaca agtacagctg 4381 caagcggtgc cagctcacct tcggccgggg gaaggagtac ctgaagcaca tcatggaggt 4441 gcacaaggag aagggctatg gctgcagcat ctgcaaccgg cgctttgcac tgaaggccac 4501 ctaccacgcc cacatggtca tccaccgtga aaacctgccg gaccccaacg tgcagaagta 4561 catccacccc tgcgagatct gcgggcggat cttcaacagc atcgggaacc tggagcgcca 4621 caagctcatc cacacaggtg tgaagagcca cgcctgcgag cagtgtggga agtcctttgc 4681 caggaaggac atgctgaagg agcacatgcg tgtgcacgac aatgtccgcg agtacctgtg 4741 tgccgagtgt gggaaaggca tgaagaccaa gcacgcgctg cgccaccaca tgaagctgca 4801 caagggcatc aaggagtacg agtgcaagga gtgccaccgc aggttcgcgc agaaggtcaa 4861 catgctcaag cactgcaagc ggcacacggg gattaaagat ttcatgtgtg aattgtgtgg 4921 gaagacattc agcgagagga acaccatgga gacccacaag ctcatccaca cagtgggcaa 4981 gcagtggacg tgctccgtgt gcgacaagaa gtacgtgacc gagtacatgc tgcagaagca 5041 cgttcagctc acacacgaca aggtggaggc gcagagctgc cagctgtgcg ggaccaaggt 5101 gtccaccagg gcctccatga gccgacacat gcggcgcaag caccccgagg tgctcgcggt 5161 gaggatcgat gacctggacc acctcccgga gaccaccacc atcgacgcct cctccattgg 5221 catcgtccag cctgagctga ctctggagca ggaggatttg gccgaaggga agcacgggaa 5281 agctgccaag cgaagtcaca agagaaagca gaagccagaa gaggaggcgg gtgctccggt 5341 gcccgaggac gccaccttca gcgaatactc agagaaagag acggagttca caggcagtgt 5401 aggcgacgag accaattccg cagtacagag cattcagcag gtagtggtga ccctgggtga 5461 cccaaatgtg accacaccat cgagctcagt cggcttaacc aacatcaccg tgacccccat 5521 caccactgcg gccgcgactc agtttaccaa tctccagccg gtggccgtgg ggcaccttac 5581 cacccctgaa cgccagttac agctggacaa ctcaatcctg accgtgacct ttgataccgt 5641 cagcggctct gccatgttgc acaaccgcca aaatgacgtc cagatccacc cccagccgga 5701 agcctcgaac ccacagtctg tggcccattt catcaacctg acgaccctgg tcaactccat 5761 cacgcccctg gggagccagc ttagtgacca gcacccgctc acgtggcggg cagtgcccca 5821 gactgacgtc ttgccaccct cgcagccgca ggcaccccca cagcaggcgg cccagcccca 5881 ggtgcaggcg gagcagcagc agcagcagat gtacagctac ggatccTACC CATACGATGT 5941 TCCAGATTAC GCTTAGgcgg ccgctgggta ggggaggcgc ttttcccaag gcagtctgga 6001 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc 6061 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg 6121 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc 6181 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct 6241 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct 6301 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg 6361 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc 6421 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc 6481 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 6541 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 6601 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 6661 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 6721 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 6781 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 6841 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 6901 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 6961 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 7021 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 7081 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 7141 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 7201 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 7261 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 7321 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 7381 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 7441 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 7501 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 7561 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 7621 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 7681 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 7741 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 7801 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 7861 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 7921 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 7981 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 8041 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 8101 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 8161 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 8221 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 8281 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 8341 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 8401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 8461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 8521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 8581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 8641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 8701 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 8761 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 8821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 8881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 8941 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 9001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 9061 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 9121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 9181 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 9241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 9301 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 9361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 9421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 9481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 9541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 9601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 9661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 9721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 9781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 9841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 9901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 9961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 10021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 10081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 10141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 10201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 10261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 10321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 10381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 10441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 10501 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 10561 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 10621 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 10681 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 10741 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 10801 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 10861 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 10921 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 10981 cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???