pLV3-H1-2O2-MAT2A-shRNA3-EGFP-TetR-Puro plasmid

PVT96354

pLV3-H1-2O2-MAT2A-shRNA3-EGFP-TetR-Puro plasmid Information

Plasmid name: pLV3-H1-2O2-MAT2A(human)-shRNA3-EGFP-TetR-Puro
Alias: GTTCAGGTCTCTTATGCTATT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence,Tetracycline induction
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: H1-2O2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: H1
Reverse primer:
Copy number: High
Induction:

pLV3-H1-2O2-MAT2A-shRNA3-EGFP-TetR-Puro plasmid Description

pLV3-H1-2O2-MAT2A-shRNA3-EGFP-TetR-Puro This is a plasmid suitable for interference silencing in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV3-H1-2O2-MAT2A-shRNA3-EGFP-TetR-Puro plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt 541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt 601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct 661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa 721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt 781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat 841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat 901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc 961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga 1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt 1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat 1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag 1801 cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa cgttactggc 1861 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 1921 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 1981 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 2041 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 2101 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 2161 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 2221 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 2281 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 2341 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 2401 ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 2461 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 2521 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 2581 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 2641 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 2701 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 2761 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 2821 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 2881 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 2941 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 3001 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc 3061 acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 3121 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 3181 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 3241 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 3301 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 3361 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 3421 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 3481 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3541 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 3601 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 3661 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 3721 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt 3781 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 3841 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 3901 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc 3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4141 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4261 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4381 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 4441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 4501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 4561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 4621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 4681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 4741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 4801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 4861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 4921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 4981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 5041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 5101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 5161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 5221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 5281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 5341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 5401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 5461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 5521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 5581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 5641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5701 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 5761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 5821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 5881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 6001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 6061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 6121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 6181 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 6241 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 6301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 6361 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 6421 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 6481 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 6541 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag 6601 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc 6661 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc 6721 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg 6781 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta 6841 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc 6901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 6961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 7021 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg 7081 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc 7141 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg 7201 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc 7261 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac 7321 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac 7381 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag 7441 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca 7501 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc 7561 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta 7621 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga 7681 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc 7741 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata 7801 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc 7861 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag 7921 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg 7981 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg 8041 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa 8101 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt 8161 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata 8221 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt 8281 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac 8341 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta 8401 gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc aatatttgca 8461 tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact 8521 tataagttcc ctatcagtga tagagacacc ggGTTCAGGT CTCTTATGCT ATTctcgagA 8581 ATAGCATAAG AGACCTGAAC TTTTTTgaat tcCGCGTTAC ATAACTTACG GTAAATGGCC 8641 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA 8701 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG 8761 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG 8821 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT 8881 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA 8941 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG 9001 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT 9061 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 9121 CTAATCGTCG ACGATTCGTC AGAATCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGCTG 9181 TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC 9241 AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC 9301 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC 9361 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC 9421 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG 9481 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC 9541 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC 9601 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC 9661 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC 9721 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG 9781 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC 9841 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAATTCt cgacctcgag acaaatggca 9901 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 9961 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 10021 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag 10081 gggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???