pLV3-H1-2O2-RPPH1-lncRNA-shRNA3-TetR-EGFP-Puro plasmid

PVT90770

pLV3-H1-2O2-RPPH1-lncRNA-shRNA3-TetR-EGFP-Puro plasmid Information

Plasmid name: pLV3-H1-2O2-RPPH1(human)-lncRNA-shRNA3-TetR-EGFP-Puro
Alias: CATAACCCAATTCAGACTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence,Tetracycline induction
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: H1-2O2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: H1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV3-H1-2O2-RPPH1-lncRNA-shRNA3-TetR-EGFP-Puro plasmid Description

pLV3-H1-2O2-RPPH1-lncRNA-shRNA3-TetR-EGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV3-H1-2O2-RPPH1-lncRNA-shRNA3-TetR-EGFP-Puro plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt 541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt 601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct 661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa 721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt 781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat 841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat 901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc 961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga 1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt 1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat 1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggg gaggtggcag 1801 cggtggcggt cagctgttga attttgacct tcttaaactt gcgggagacg tcgagtccaa 1861 ccctgggccc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 1921 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 1981 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 2041 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 2101 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 2161 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 2221 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 2281 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 2341 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 2401 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 2461 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 2521 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 2581 cgagctgtac aagtaacccg ggagttctag ggatctgccc ctctccctcc ccccccccta 2641 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 2701 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 2761 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 2821 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 2881 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 2941 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 3001 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 3061 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 3121 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 3181 cacgatgata aggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc 3241 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 3301 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 3361 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 3421 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 3481 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 3541 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 3601 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 3661 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 3721 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 3781 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc 3841 gaaaggagcg cacgacccca tgcatcggta cctttaagac caatgactta caaggcagct 3901 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 3961 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 4021 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 4081 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 4141 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 4201 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 4261 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 4321 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 4381 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4441 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 4501 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 4561 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 4621 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 4681 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 4741 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 4801 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 4861 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 4921 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 4981 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 5041 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 5101 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 5161 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 5221 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 5281 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 5341 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 5401 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 5461 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 5521 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 5581 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 5641 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 5701 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 5761 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 5821 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 5881 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 5941 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 6001 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 6061 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 6121 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 6181 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 6241 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 6301 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 6361 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 6421 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 6481 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 6541 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 6601 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 6661 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 6721 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 6781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 6841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 6901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 6961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 7021 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 7081 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 7141 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 7201 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 7261 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 7321 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac 7381 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct 7441 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 7501 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac 7561 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat 7621 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg 7681 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7741 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7801 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg 7861 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg 7921 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag 7981 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat 8041 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag 8101 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca 8161 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata 8221 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga 8281 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc 8341 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata 8401 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 8461 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 8521 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 8581 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 8641 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 8701 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 8761 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc 8821 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct 8881 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata 8941 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat 9001 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 9061 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 9121 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat 9181 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 9241 caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tccctatcag 9301 tgatagagac ttataagttc cctatcagtg atagagacac cggCATAACC CAATTCAGAC 9361 TAttcaagag aTAGTCTGAA TTGGGTTATG ttttttgaat tctcgacctc gagacaaatg 9421 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 9481 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 9541 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc 9601 gaggggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.