pLV3-U6-Ccl20-shRNA1-mCherry-Neo plasmid

PVT98138

pLV3-U6-Ccl20-shRNA1-mCherry-Neo plasmid Information

Plasmid name: pLV3-U6-Ccl20(mouse)-shRNA1-mCherry-Neo
Alias: CCAAAGCAGAACTGGGTGAAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Ccl20-shRNA1-mCherry-Neo plasmid Description

pLV3-U6-Ccl20-shRNA1-mCherry-Neo This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-Ccl20-shRNA1-mCherry-Neo plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccATGGT GAGCAAGGGC GAGGAGGATA 541 ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG 601 GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG 661 CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC 721 AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA 781 AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG 841 TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC 901 TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG 961 AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA 1021 GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA 1081 AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC 1141 ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG 1201 GCGGCATGGA CGAGCTGTAC AAGGAATTTG GCAGCGGCCA GTGCACCAAC TATGCCCTGC 1261 TGAAACTGGC TGGCGATGTG GAAAGCAACC CCGGACCCat ggctgaacaa gatggattgc 1321 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 1381 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 1441 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 1501 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 1561 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 1621 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 1681 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 1741 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 1801 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 1861 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 1921 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 1981 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 2041 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgacgcccgc 2101 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa 2161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 2221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 2281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 2341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 2401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 2461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 2521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 2581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 2641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 2701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 2761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 2821 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 2881 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 2941 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 3001 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 3061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 3121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 3181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 3241 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 3301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 3361 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 3421 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 3481 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 3541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 3601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 3661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 3721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 3781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 3841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 3901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 3961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 4021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 4081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 4141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 4201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 4261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 4321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 4381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 4441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 4501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 4561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 4681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 4741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 4801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 4861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 4921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 4981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 5041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 5101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 5161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 5221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 5281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 5341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 5401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 5461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 5521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 5581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 5641 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt 5701 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 5761 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 5821 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 5881 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 5941 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 6001 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6061 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 6121 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 6181 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 6241 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 6301 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 6361 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 6421 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 6481 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 6541 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 6601 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 6661 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 6721 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 6781 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 6841 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 6901 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 6961 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 7021 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 7081 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 7141 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 7201 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 7261 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 7321 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 7381 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 7441 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc 7501 acgagactag cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc 7561 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa 7621 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc 7681 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt 7741 cgatttcttg gctttatata tcttgtggaa aggacgaaac ACCGGCCAAA GCAGAACTGG 7801 GTGAAAttca agagaTTTCA CCCAGTTCTG CTTTGGtttt ttGAATTCtc gacctcgaga 7861 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca 7921 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa 7981 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg 8041 cggctcgagg ggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???