pLV3-U6-CERS1-shRNA3-EF1a-Puro plasmid

PVT92766

pLV3-U6-CERS1-shRNA3-EF1a-Puro plasmid Information

Plasmid name: pLV3-U6-CERS1(human)-shRNA3-EF1a-Puro
Alias: ccatctacgctacgctataca;
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-CERS1-shRNA3-EF1a-Puro plasmid Description

pLV3-U6-CERS1-shRNA3-EF1a-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-CERS1-shRNA3-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTATCGA TCTGGGCAGG AAGAGGGCCT 1981 ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG 2041 AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA 2101 ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC 2161 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGATC 2221 CGccatctac gctacgctat acaTTCAAGA GAtgtatagc gtagcgtaga tggTTTTTTG 2281 AATTCgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2341 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 2401 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2461 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2521 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 2581 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 2641 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 2701 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 2761 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 2821 accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 2881 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 2941 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 3001 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 3061 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 3121 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 3181 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 3241 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 3301 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 3361 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 3421 gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga 3481 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 3541 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 3601 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 3661 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 3721 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 3781 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 3841 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 3901 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3961 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 4021 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 4081 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 4141 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 4201 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 4261 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 4321 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 4381 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 4441 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 4501 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 4561 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4621 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 4681 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 4741 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 4801 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 4861 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 4921 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 4981 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5041 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5101 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5161 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5221 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5281 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 5341 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5401 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5461 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5521 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5581 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5641 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5701 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5761 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5821 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5881 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5941 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6001 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6061 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6121 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6181 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6241 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6301 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6361 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6421 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6481 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6541 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6601 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6661 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6721 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6781 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6841 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 6901 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 6961 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 7021 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 7081 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 7141 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 7201 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 7261 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 7321 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???